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Hi there,<div><br></div><div>Here is the information for an activated caspase-3 antibody that I have used successfully for whole-mount IF on zebrafish: BD Pharmingen Rabbit Anti Active Caspase-3, catalog number 559565.</div><div><br></div><div>Good luck!</div><div>Kristen Kwan<br><div><br><div><br></div><blockquote type="cite"><div><div><div>Begin forwarded message:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" color="#000000" style="font: 12.0px Helvetica; color: #000000"><b>From: </b></font><font face="Helvetica" size="3" style="font: 12.0px Helvetica">Weibin Zhou <<a href="mailto:weibinz@med.umich.edu">weibinz@med.umich.edu</a>></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" color="#000000" style="font: 12.0px Helvetica; color: #000000"><b>Date: </b></font><font face="Helvetica" size="3" style="font: 12.0px Helvetica">June 10, 2009 1:18:10 PM MDT</font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" color="#000000" style="font: 12.0px Helvetica; color: #000000"><b>To: </b></font><font face="Helvetica" size="3" style="font: 12.0px Helvetica">"<a href="mailto:zbrafish@magpie.bio.indiana.edu">zbrafish@magpie.bio.indiana.edu</a>" <<a href="mailto:zbrafish@magpie.bio.indiana.edu">zbrafish@magpie.bio.indiana.edu</a>></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Helvetica" size="3" color="#000000" style="font: 12.0px Helvetica; color: #000000"><b>Subject: </b></font><font face="Helvetica" size="3" style="font: 12.0px Helvetica"><b>[Zbrafish] caspase antibody</b></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; min-height: 14px; "><br></div> </div><div>Hi, <br>could someone recommend an antibody for activated caspase-3 that works on zebrafish? We would like to examine apoptotic cells with whole-mount IF. Thank you very much.<br><br><br><br>**********************************************************<br>Electronic Mail is not secure, may not be read every day, and should not be used for urgent or sensitive issues<br><br>_______________________________________________<br>Zbrafish mailing list<br><a href="mailto:Zbrafish@net.bio.net">Zbrafish@net.bio.net</a><br><a href="http://www.bio.net/biomail/listinfo/zbrafish">http://www.bio.net/biomail/listinfo/zbrafish</a><br></div></blockquote></div><br></div></blockquote></div><br><div> <span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div>Kristen M. Kwan, PhD</div><div>Department of Neurobiology and Anatomy</div><div>University of Utah</div><div>409 Wintrobe</div><div>20 N 1900 E</div><div>Salt Lake City, UT 84112</div><div>(801) 585-1702</div><div><br class="khtml-block-placeholder"></div><br class="Apple-interchange-newline"></span> </div><br></div></body></html>
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