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<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi Sarah,<div><br></div><div>If you are talking about live 5 dpf fish, you will need to mount in some way; otherwise, they will float up because of their inflated swim bladders. Anesthetizing with tricaine and mounting in low-melt agarose is simple and effective.</div><div><br></div><div>best wishes,</div><div>Chi-Bin Chien</div><div><br><div apple-content-edited="true"> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div><div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Monaco" size="1" style="font: normal normal normal 9px/normal Monaco; ">< Dept. Neurobiology and Anatomy | office: 1-801-585-1701 ></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Monaco" size="1" style="font: normal normal normal 9px/normal Monaco; ">< Univ. Utah Medical Center | lab: 1-801-585-1702 ></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Monaco" size="1" style="font: normal normal normal 9px/normal Monaco; ">< 20 North 1900 East, 401 MREB | fax: 1-801-581-4233 ></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Monaco" size="1" style="font: normal normal normal 9px/normal Monaco; ">< Salt Lake City, UT 84132 | <a href="mailto:chi-bin.chien@neuro.utah.edu">chi-bin.chien@neuro.utah.edu</a> ></font></div><div style="margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><font face="Monaco" size="1" style="font: normal normal normal 9px/normal Monaco; ">< <a href="http://chien.neuro.utah.edu">http://chien.neuro.utah.edu</a> ></font></div></div></div></div></span></span></div></span></div></span> </div><br><div><div>On May 22, 2009, at 6:27 AM, FritzXC23 wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div>Hi All!<br><br>My name is Sarah Fritz and I am undergraduate student from Gettysburg<br>College. I am currently working on a research project that involves<br>imaging fluorescent zebrafish hair cells. I am wondering if anyone has<br>suggestions about the best way to get 5 dpf fish to lay on their sides<br>without having to mount them. Thanks!<br><br>Sarah Fritz<br><br>_______________________________________________<br>Zbrafish mailing list<br><a href="mailto:Zbrafish@net.bio.net">Zbrafish@net.bio.net</a><br>http://www.bio.net/biomail/listinfo/zbrafish<br></div></blockquote></div><br></div></body></html>
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