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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'>Hi Alice,<o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'><o:p>&nbsp;</o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'>Lines can undergo silencing across generations.&nbsp; We always
choose the brightest GFP carriers when we incross or outcross and only raise
those.&nbsp; See the paper below for some more details.<o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'><o:p>&nbsp;</o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'>Becky<o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'><o:p>&nbsp;</o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'><o:p>&nbsp;</o:p></span></p>

<p class=title1 style='line-height:21.6pt;background:white'><span
style='font-size:11.0pt;font-family:"Arial","sans-serif"'><a
href="http://www.ncbi.nlm.nih.gov/pubmed/19433629"><span style='color:#2222CC'>Transcriptional
silencing and reactivation in transgenic zebrafish.</span></a> </span><span
style='font-size:10.5pt;font-family:"Arial","sans-serif"'>Goll MG, Anderson R,
Stainier DY, Spradling AC, Halpern ME.</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif"'><o:p></o:p></span></p>

<p class=aux1 style='background:white'><span class=jrnl><span style='font-size:
9.0pt;font-family:"Arial","sans-serif"'>Genetics</span></span><span class=src1><span
style='font-size:9.0pt;font-family:"Arial","sans-serif"'>. 2009
Jul;182(3):747-55. Epub 2009 May 11.</span></span><span style='font-size:9.0pt;
font-family:"Arial","sans-serif"'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'><o:p>&nbsp;</o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'><o:p>&nbsp;</o:p></span></p>

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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>---------------------------------------------------</span><span
style='font-size:11.0pt;font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Rebecca D. Burdine, Ph.D.</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Assistant Professor</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Dept. of Molecular Biology</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Princeton University</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Washington Road Mof 433</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Princeton, NJ 08544&nbsp;</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Arial","sans-serif";
color:black'>&nbsp;</span><span style='font-size:11.0pt;font-family:"Arial","sans-serif";
color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Phone:&nbsp;(609) 258-7515</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Fax: (609) 258-6730</span><span style='font-size:11.0pt;
font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Email: <a href="mailto:rburdine@princeton.edu" target="_blank"><span
style='color:blue'>rburdine@princeton.edu</span></a></span><span
style='font-size:11.0pt;font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:black'>Admin Assistant: Anna Schmedel (609) 258-5028</span><span
style='font-size:11.0pt;font-family:"Arial","sans-serif";color:#1F497D'><o:p></o:p></span></p>

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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif";
color:#1F497D'><o:p>&nbsp;</o:p></span></p>

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<p class=MsoNormal><b><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>From:</span></b><span
style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>
zbrafish-bounces@oat.bio.indiana.edu
[mailto:zbrafish-bounces@oat.bio.indiana.edu] <b>On Behalf Of </b>Shia, Alice K
H<br>
<b>Sent:</b> Thursday, October 28, 2010 6:59 AM<br>
<b>To:</b> 'zbrafish@net.bio.net'<br>
<b>Subject:</b> [Zbrafish] Stability of zebrafish GFP transgenics<o:p></o:p></span></p>

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<p class=MsoNormal><o:p>&nbsp;</o:p></p>

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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Hi
all,</span><o:p></o:p></p>

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<p class=MsoNormal>&nbsp;<o:p></o:p></p>

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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>We
have something weird going on in our lab at the moment. Homozygous transgenic
lines have started to lose their gfp signal or some of the lines have become
more and more heterogenous in their gfp expression in embryos.</span><o:p></o:p></p>

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<p class=MsoNormal>&nbsp;<o:p></o:p></p>

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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Does
everyone select their rearing embryos at every generation? These are published
lines and were fine when we started using them. Some have been reared for 2-3
generations (inbreeding cross) and some have been outcrossed and reselected.
The lines have arrived from different labs.</span><o:p></o:p></p>

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<p class=MsoNormal>&nbsp;<o:p></o:p></p>

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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Any
advice would be helpful.</span><o:p></o:p></p>

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<p class=MsoNormal>&nbsp;<o:p></o:p></p>

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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Many
thanks</span><o:p></o:p></p>

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<p class=MsoNormal>&nbsp;<o:p></o:p></p>

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<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Arial","sans-serif"'>Alice
shia</span><o:p></o:p></p>

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<p class=MsoNormal>&nbsp;<o:p></o:p></p>

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<p class=MsoNormal>&nbsp;<o:p></o:p></p>

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