From owner-7tms_r@net.bio.net Fri Nov 01 22:00:00 1996 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: GPCRDB temporarily down Date: 2 Nov 1996 00:24:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611020823.JAA00762@nu> NNTP-Posting-Host: net.bio.net The GPCRDB server suffered a crash of the hard disk. A new disk has been ordered, and we expect to be available again next weekend (Nov 9 or 10). Sorry for the inconvenience. Gert Vriend From owner-7tms_r@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: Fwd: GHRH receptor gene exon/intron organization Date: 4 Nov 1996 01:35:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611040934.KAA23556@nu> NNTP-Posting-Host: net.bio.net ------- start of forwarded message (RFC 934 encapsulation) ------- Received: from skua.embl-heidelberg.de (skua.EMBL-Heidelberg.DE [192.54.41.199]) by stork.EMBL-Heidelberg.DE (8.7.1/8.7.1) with ESMTP id VAA11615 for ; Sun, 3 Nov 1996 21:59:05 +0100 (MET) Received: by skua.embl-heidelberg.de (8.7.1) id UAA17508; Sun, 3 Nov 1996 20:59:05 GMT Message-Id: <199611032059.UAA17508@skua.embl-heidelberg.de> Errors-To: tulpakov@online.ru Sender: tulpakov@online.ru X-Mail-Gateway: Doug's WWW Mail Gateway 1.4 X-Real-Host-From: disc.dna.affrc.go.jp From: Anatoly Tiulpakov To: vriend Subject: GHRH receptor gene exon/intron organization Date: Sun, 3 Nov 1996 20:59:05 GMT Reply-To: tulpakov@online.ru I received this question privately, can anybody help? Please reply to: tulpakov@online.ru Thanks Gert Vriend Dear Sir/Madam, I am looking for exon/intron organization of GHRH receptor gene. I would greatly appreciate receiving any available information on this subject. Many thanks in advance. Sincerely, Anatoly Tiulpakov Pediatric Unit Endocr Res Center Moscow, Russia ------- end ------- From owner-7tms_r@net.bio.net Sun Nov 03 22:00:00 1996 Path: biosci!Lilly.com!SHARP_JOHN_D From: SHARP_JOHN_D@Lilly.com ("J.D.SHARP 317-276-4268 DROP 04") Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 4 Nov 1996 04:48:48 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <9417470704111996.A63652.MCVAX4.11AB21EF0A00*@MHS> NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Tue Nov 05 22:00:00 1996 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: HEK-293 cells Date: 6 Nov 1996 08:41:56 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 34 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Rob Leurs (leurs@chem.vu.nl) wrote: >To study 7TM receptors and to measure their activity by use of cAMP >measurements we express these receptors in HEK-293 cells. To improve >attachment of these cells we use Pronectin F attachment factor. Since the >attachment of these cells is still not very good I want to know if their >are any other methods to improve the attachment of HEK-293 cells. I would >also be willing to switch to another cell system with large cAMP responses. >Any protocols or suggestions for other cells ?? I know that some investigators coat their dishes with gelatin r=prior top plating HEK293 cells. I don't know personally whether this is any better than pronectin F. However, when we tested commercially available pronectin-coated dishes, we found that there was not a big improvement in attachment. AJW ------------------------------------------------------------------------ __ __ __ A. John Watson, Ph.D. /__/__/__/\ Bristol-Myers Squibb Company /__/__/__/\/\ Pharmaceutical Reseach Institute /__/__/__/\/\/\ CNS Molecular Biology, Dept. 405 \__\__\__\/\/\/ 5 Research Parkway \__\__\__\/\/ Wallingford, CT 06492 \__\__\__\/ watson_j@bms.com | 203.284.6745 voice | Standard Disclaimers Apply 203.284.7569 fax | ------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Tue Nov 05 22:00:00 1996 Path: biosci!MSMAIL.BMS.COM!Mahle#m#_Cathy_D#d#.Wallingford_Mail_Server From: Mahle#m#_Cathy_D#d#.Wallingford_Mail_Server@MSMAIL.BMS.COM ("Mahle, Cathy D.") Newsgroups: bionet.molbio.proteins.7tms_r Subject: RE: HEK-293 cells Date: 6 Nov 1996 08:12:51 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 72 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Rob, We also have 293 cells that do not adhere well. We have tried in the past to improve adherence by using many things (collagen I, collagen IV, fibronectin, laminin, and poly-d-lysine coated plates). None of these techniques remarkedly improved adherence. We later learned that someone within our department has 293 cells that actually adhere quite well. Aside from trying different sources of 293 cells, we have found CHO cells and 3T3 cells work quite well for cAMP assays. Cathy Mahle CNS Drug Discovery Bristol-Myers Squibb Wallingford, CT mahle@bms.com _______________________________________________________________________________ From: Rob Leurs on Wed, Nov 6, 1996 9:23 AM Subject: HEK-293 cells To: 7tms_r@net.bio.net To study 7TM receptors and to measure their activity by use of cAMP measurements we express these receptors in HEK-293 cells. To improve attachment of these cells we use Pronectin F attachment factor. Since the attachment of these cells is still not very good I want to know if their are any other methods to improve the attachment of HEK-293 cells. I would also be willing to switch to another cell system with large cAMP responses. Any protocols or suggestions for other cells ?? ****************************************************************************** Rob Leurs, Ph.D Leiden/Amsterdam Center for Drug Research Division of Medicinal Chemistry Department of Pharmacochemistry, Vrije Universiteit De Boelelaan 1083, 1081 HV Amsterdam the Netherlands fax: 31204447610 tel: 31204447579 leurs@chem.vu.nl ------------------ RFC822 Header Follows ------------------ Received: by msmail.bms.com with ADMIN;6 Nov 1996 09:22:29 -0400 Received: from conan.bms.com by cliff.bms.com (PMDF V5.0-7 #15142) id <01IBIT6KV1DS00VCCN@cliff.bms.com> for Mahle#m#_Cathy_D#d#@msmail.bms.com; Wed, 06 Nov 1996 10:21:57 -0500 (EST) Received: from bms.com by bms.com (PMDF V4.3-7 #15142) id <01IBIR34ECSY8Z5NCE@bms.com>; Wed, 06 Nov 1996 09:21:51 -0500 (EST) Received: from cliff.bms.com by bms.com (PMDF V4.3-7 #15142) id <01IBIR2FP4U8QO9PMX@bms.com>; Wed, 06 Nov 1996 09:21:45 -0500 (EST) Received: from directory-daemon by cliff.bms.com (PMDF V5.0-7 #15142) id <01IBIT5ZRMBK00VC1B@cliff.bms.com>; Wed, 06 Nov 1996 10:21:26 -0500 (EST) Received: from net.bio.net by cliff.bms.com (PMDF V5.0-7 #15142) id <01IBIT5NG5V400URZ0@cliff.bms.com>; Wed, 06 Nov 1996 10:21:15 -0500 (EST) Received: (from daemon@localhost) by net.bio.net (8.6.12/8.6.6) id EAA18770; Wed, 06 Nov 1996 04:57:20 -0800 Received: (from news@localhost) by net.bio.net (8.6.12/8.6.6) id EAA18766; Wed, 06 Nov 1996 04:57:13 -0800 Date: Wed, 06 Nov 1996 12:54:40 +0000 From: leurs@chem.vu.nl (Rob Leurs) Subject: HEK-293 cells Sender: lpddist@mserv1.dl.ac.uk Resent-to: Mahle#m#_Cathy_D#d#@msmail.bms.com To: 7tms_r@net.bio.net Resent-message-id: <01IBIR34EW388Z5NCE@bms.com> Message-id: <55q1qg$5uo@mserv1.dl.ac.uk> X-VMS-To: IN%"7tms_r@net.bio.NET" Content-transfer-encoding: 7BIT Original-To: 7tms_r@dl.ac.uk From owner-7tms_r@net.bio.net Tue Nov 05 22:00:00 1996 Path: biosci!daresbury!not-for-mail From: leurs@chem.vu.nl (Rob Leurs) Newsgroups: bionet.molbio.proteins.7tms_r Subject: HEK-293 cells Date: 6 Nov 1996 12:54:40 -0000 Lines: 21 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <55q1qg$5uo@mserv1.dl.ac.uk> Original-To: 7tms_r@dl.ac.uk To study 7TM receptors and to measure their activity by use of cAMP measurements we express these receptors in HEK-293 cells. To improve attachment of these cells we use Pronectin F attachment factor. Since the attachment of these cells is still not very good I want to know if their are any other methods to improve the attachment of HEK-293 cells. I would also be willing to switch to another cell system with large cAMP responses. Any protocols or suggestions for other cells ?? ****************************************************************************** Rob Leurs, Ph.D Leiden/Amsterdam Center for Drug Research Division of Medicinal Chemistry Department of Pharmacochemistry, Vrije Universiteit De Boelelaan 1083, 1081 HV Amsterdam the Netherlands fax: 31204447610 tel: 31204447579 leurs@chem.vu.nl From owner-7tms_r@net.bio.net Wed Nov 06 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!newsfeed.internetmci.com!news.ycc.yale.edu!news From: Emmanouil Skoufos Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Fwd: GHRH receptor gene exon/intron organization Date: Thu, 07 Nov 1996 16:30:24 -0800 Organization: Yale University School of Medicine Lines: 37 Message-ID: <32827F20.2781@pantheon.yale.edu> References: <199611040934.KAA23556@nu> NNTP-Posting-Host: miris.med.yale.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX 5.2 IP20) To: tulpakov@online.ru Gert.Vriend@EMBL-HEIDELBERG.DE wrote: > > ------- start of forwarded message (RFC 934 encapsulation) ------- > Received: from skua.embl-heidelberg.de (skua.EMBL-Heidelberg.DE [192.54.41.199]) by stork.EMBL-Heidelberg.DE (8.7.1/8.7.1) with ESMTP id VAA11615 for ; Sun, 3 Nov 1996 21:59:05 +0100 (MET) > Received: by skua.embl-heidelberg.de (8.7.1) id UAA17508; Sun, 3 Nov 1996 20:59:05 GMT > Message-Id: <199611032059.UAA17508@skua.embl-heidelberg.de> > Errors-To: tulpakov@online.ru > Sender: tulpakov@online.ru > X-Mail-Gateway: Doug's WWW Mail Gateway 1.4 > X-Real-Host-From: disc.dna.affrc.go.jp > Dear Sir/Madam, > I am looking for exon/intron organization of GHRH receptor gene. I would > greatly appreciate receiving any available information on this subject. > Many thanks in advance. Is the receptor in question the Gonadotropin Releasing Hormone (GnRH) receptor? If yes, its genomic stucture is not known. Emmanuel ---- Emmanuel Skoufos, Ph.D. Yale University School of Medicine Section of Neurobiology 333 Cedar Street, 236 FMB New Haven, CT 06520 e-mail:emmanouil.skoufos@yale.edu Phone: (203) 785-4336 Fax: (203) 785-6990 gene discovery page: http://www.geocities.com/CapeCanaveral/1915/gdp.html ORDB: http://senselab.med.yale.edu/ORDB From owner-7tms_r@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!mail.nrgn.com!rbrodbeck From: rbrodbeck@mail.nrgn.com ("Robbin Brodbeck") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Endogenous G protein expres Date: 8 Nov 1996 05:52:28 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 49 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net GPCR group: On 10/18/96 I wrote, In the interest of better understanding the artificial expression systems we all use to express our various GPCR's, i.e., CHO, Cos, Sf9, etc., I am wondering about G-protein expression. There has been numerous postings in the last month or so dealing with the endogenous receptors expressed in most of the common cell lines used in research, but nothing with respect to the endogenous GPs found! This is obviously a very important issue to us all! I'm wondering if anyone knows of any data on the characterization of GP content for either common research cell lines, or various tissues in the body with particular attention to regions of the brain I recieved over 10 inquiries requesting a summary of the data but very little in the way of data...maybe no one knows the answer to this question? Here is the list of replies I recieved: 1) Rick Neubig wrote: You are likely to find some different answers depending on the particular CHO strain (and maybe even the particular lab clone) used. We looked at Gi-class subtypes and felt that our data with CHO-K1 were most consistent with alpha-i2 and alpha-i3 but not alpha-i1 or alpha-o being present. See 1. Gerhardt, M.A. and Neubig, R.R. Multiple Gi subtypes couple to a single effector mechanism. Mol.Pharmacol 40:707-711, 1991. The antisera we had available weren't the most specific so there may well be better data since then. 2) Bryan Roth wrote: NIH 3T3 cells express Gq. Thats all! If I hear any more I'll post it as I hear it. Robbin Brodbeck Neurogen Corp. From owner-7tms_r@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!pr.cyanamid.com!ramaswam From: ramaswam@pr.cyanamid.com (Ramaswamy Nilakantam) Newsgroups: bionet.molbio.proteins.7tms_r Subject: helical wheel Date: 8 Nov 1996 07:50:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 29 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611081547.AA274268023@primail.pr.cyanamid.com> NNTP-Posting-Host: net.bio.net > Dear collegues, > Could someone indicate me whether the following types of programs are availabl e > somewhere: > 1)one that draws an helical wheel model starting from the aminoacid sequence > of an helix. > 2)one that uses a transmembrane protein aminoacid sequence and limits of > putative transmembrane helices as input and generates a drawing of the > transmembrane folding. > Thanks in advance. > > Michel > > M. Seigneuret > Lab. de Biophysique Cellulaire et RMN > Universite Paris 7 > France > > > I am sure there are many programs available for plotting helical wheels, but one I am personally aware of is part of the Wisconsin Sequence Analysis Package which you can get from Genetics Computer Group in Madison, Wisconsin. Phone: (608) 231-5200, FAX (608) 231-5202, e-mail help@GCG.Com. Ramaswamy Nilakantan Wyeth-Ayerst Research, Pearl River, NY From owner-7tms_r@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!news.sgi.com!esiee.fr!jussieu.fr!Newsmaster From: Michel SEIGNEURET Newsgroups: bionet.molbio.proteins.7tms_r Subject: drawing of transmembrane helices Date: 8 Nov 1996 09:07:20 GMT Organization: Universites Paris VI/Paris VII - France Lines: 17 Message-ID: <55ut88$g4l@vishnu.jussieu.fr> NNTP-Posting-Host: mood.biophy.jussieu.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 1.1N (X11; I; IRIX 5.3 IP20) X-URL: news:bionet.molbio.proteins.7tms_r Dear collegues, Could someone indicate me whether the following types of programs are available somewhere: 1)one that draws an helical wheel model starting from the aminoacid sequence of an helix. 2)one that uses a transmembrane protein aminoacid sequence and limits of putative transmembrane helices as input and generates a drawing of the transmembrane folding. Thanks in advance. Michel M. Seigneuret Lab. de Biophysique Cellulaire et RMN Universite Paris 7 France From owner-7tms_r@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!www.nntp.primenet.com!nntp.primenet.com!nntp.uio.no!nntp.zit.th-darmstadt.de!fu-berlin.de!informatik.tu-muenchen.de!lrz-muenchen.de!uni-erlangen.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: drawing of transmembrane helices Date: 8 Nov 1996 13:24:26 GMT Organization: University of Wuerzburg, Germany Lines: 22 Message-ID: <55vcaa$gcl@winx03.informatik.uni-wuerzburg.de> References: <55ut88$g4l@vishnu.jussieu.fr> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Michel SEIGNEURET (michel) wrote: > Could someone indicate me whether the following types of programs are > available somewhere: > 1)one that draws an helical wheel model starting from the aminoacid sequence > of an helix. GCG offers HELICALWHEEL which does exactly that. I've been told that there is a freeware program for the Mac somewhere out there which does similar things. > 2)one that uses a transmembrane protein aminoacid sequence and limits of > putative transmembrane helices as input and generates a drawing of the > transmembrane folding. I am not aware of any such program in the public domain. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Thu Nov 07 22:00:00 1996 Path: biosci!mail.nrgn.com!rbrodbeck From: rbrodbeck@mail.nrgn.com ("Robbin Brodbeck") Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 8 Nov 1996 08:24:24 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 48 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net GPCR group: On 10/18/96 I wrote, In the interest of better understanding the artificial expression systems we all use to express our various GPCR's, i.e., CHO, Cos, Sf9, etc., I am wondering about G-protein expression. There has been numerous postings in the last month or so dealing with the endogenous receptors expressed in most of the common cell lines used in research, but nothing with respect to the endogenous GPs found! This is obviously a very important issue to us all! I'm wondering if anyone knows of any data on the characterization of GP content for either common research cell lines, or various tissues in the body with particular attention to regions of the brain I recieved over 10 inquiries requesting a summary of the data but very little in the way of data...maybe no one knows the answer to this question? Here is the list of replies I recieved: 1) Rick Neubig wrote: You are likely to find some different answers depending on the particular CHO strain (and maybe even the particular lab clone) used. We looked at Gi-class subtypes and felt that our data with CHO-K1 were most consistent with alpha-i2 and alpha-i3 but not alpha-i1 or alpha-o being present. See 1. Gerhardt, M.A. and Neubig, R.R. Multiple Gi subtypes couple to a single effector mechanism. Mol.Pharmacol 40:707-711, 1991. The antisera we had available weren't the most specific so there may well be better data since then. 2) Bryan Roth wrote: NIH 3T3 cells express Gq. Thats all! If I hear any more I'll post it as I hear it. Robbin Brodbeck Neurogen Corp. From owner-7tms_r@net.bio.net Fri Nov 08 22:00:00 1996 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: two-dimensional plots Date: 9 Nov 1996 06:34:39 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611091434.PAA01058@nu> NNTP-Posting-Host: net.bio.net The viseur software can draw snake-like diagrams etc. Their WWW home page is: http://www.lctn.u-nancy.fr/viseur/viseur.html (Be aware that that homepage is BIG, so dont sit down waiting for it to pop up in your WWW-browser, but get it shortly before lunch or so). Gert Vriend From owner-7tms_r@net.bio.net Mon Nov 11 22:00:00 1996 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Nov 1996 02:00:43 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199611121000.CAA16526@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. 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Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-7tms_r@net.bio.net Tue Nov 12 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!news.bbnplanet.com!cam-news-hub1.bbnplanet.com!uunet!in3.uu.net!newsgate.compuserve.com!news.production.compuserve.com!news From: Mike Berg <106024.34@CompuServe.COM> Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins.7tms_r,bionet.molecules.peptides Subject: HPLC solvents: filter-degass Date: 6 Nov 1996 18:04:06 GMT Organization: CompuServe, Inc. (1-800-689-0736) Lines: 10 Message-ID: <55qjum$7ot$1@mhade.production.compuserve.com> Xref: biosci bionet.molbio.methds-reagnts:51482 bionet.molbio.proteins.7tms_r:924 bionet.molecules.peptides:510 A unique all Teflon filtraton device has been developed which can filter and degass 4 liters of HPLC solvent prior to use. The device screws directly onto a 4 liter solvent bottle and works with a vacuum source. Fitration and degasssing process helps remove suspended particles which can harm delicate pump parts and clog HPLC columns and also removes dissolved gases (O2) to reduce baseline drift and improve performance in fluorescence detection. For further details contact Lazar Research Labs. Inc. by emailing service@lazarlab.com or faxing 1-213-931-1434 or viewing the Lazar web site at http://www.lazarlab.com From owner-7tms_r@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!rutgers!uwm.edu!www.nntp.primenet.com!nntp.primenet.com!mindspring!cssun.mathcs.emory.edu!news.service.emory.edu!news From: TJ Murphy Newsgroups: bionet.molbio.proteins.7tms_r Subject: Expression Cloning Date: Thu, 14 Nov 1996 16:19:29 -0500 Organization: Emory University Lines: 36 Message-ID: <328B8CE1.13BF@bimcore.emory.edu> NNTP-Posting-Host: murphy1.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) I seek your collective help. I am writing a chapter on methods for expression cloning GPCR's by the MAMMALIAN CELL EXPRESSION approach. I've dug up the following receptor types as having been cloned primarily by the mammalian cell/functional expression/ligand or 2nd messenger screen route. I'd like to be as comprehensive as possible in citing successes with this approach, and bet there are other GPCR's out there that I've missed. I am looking for examples of successful expression library screens in MAMMALIAN CELLS for GPCR's only (*not* xenopus oocytes, or ecoli, or PCR or homology, etc; and *only* GPC receptors) Here is what I have already: ET-1, AT1, k-opiod-like, ADH/V2, GLP-1, d-opiod CD97, melatonin, CRF, PACAP, glucagon, AT2, PTH/PTHRP NPY, gastrin Are you aware of any receptor types I've missed? Citations or an author name would be helpful if handy. Kindly email directly back to me. Otherwise, I'd have to read the newsgroup to collect the info, but would get distracted by fun and pleasurable posts such as Tracy's and never get the frigging chapter done. Thanks in advance. -- T.J. Murphy 404-727-2467 Assistant Professor 404-727-0365 (fax) Emory University School of Medicine medtjm@bimcore.emory.edu Department of Pharmacology 1510 Clifton Road Atlanta, GA 30322 http://www.emory.edu/PHARMACOLOGY/MURPHY/ From owner-7tms_r@net.bio.net Wed Nov 13 22:00:00 1996 Path: biosci!APOPNET.COM!webmaster From: webmaster@APOPNET.COM (APOPTOSIS Online) Newsgroups: bionet.molbio.proteins.7tms_r Subject: "APOPTOSIS Online" Site Announcement Date: 14 Nov 1996 17:47:36 -0800 Organization: ApopNet Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <328BD996.3E38@apopnet.com> NNTP-Posting-Host: net.bio.net ApopNet has established a new web site "APOPTOSIS Online: The Apoptosis Information and Communication Center". You will find "APOPTOSIS Online" on the web at: http://www.apopnet.com "APOPTOSIS Online" features an online discussion board, a resume/position depository and a content-based resource center focusing on apoptosis-related information. Access to APOPTOSIS Online is entirely free, requiring only a simple one-time member registration. I hope you find "APOPTOSIS Online" an informative and valuable resource. Webmaster, APOPTOSIS Online From owner-7tms_r@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!ESBS1.U-STRASBG.FR!Jean-Luc.Galzi From: Jean-Luc.Galzi@ESBS1.U-STRASBG.FR (Jean-Luc Galzi (ESBS)) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 15 Nov 1996 06:38:25 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I am looking for human G protein alpha-subunits cDNAs. Can anybody help me. Thanks Jean-Luc Galzi UPR CNRS 9050 Recepteurs et Proteines Membranaires Ecole Superieure des Biotechnologies Strasbourg Boulevard Sebastien Brant 67400 ILLKIRCH France tel: 88 65 52 93 88 65 52 88 Fax: 88 65 52 98 From owner-7tms_r@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!daresbury!bioftp.unibas.ch!infobiogen.fr!jussieu.fr!univ-angers.fr!ciril.fr!usenet From: Fabien Campagne Newsgroups: bionet.molbio.proteins.7tms_r Subject: Viseur program availability Date: Fri, 15 Nov 1996 16:40:11 +0000 Organization: Laboratoire de Chimie The'orique de Nancy Lines: 31 Message-ID: <328C9CEB.41C6@incm.u-nancy.fr> NNTP-Posting-Host: yellow.incm.u-nancy.fr Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (X11; I; IRIX64 6.1 IP26) Last week Gert Vriend replied to a post from Michel Seigneuret about programs that can draw a "snake-like" picture for a protein sequence. Gert gave a pointer to the Viseur program home page and I got a lot of feedback in a few days about the program availability. Here are some news about Viseur diffusion. Viseur is a program that let you manage and visualize your preferred GPCRs (please see http://www.lctn.u-nancy.fr/viseur/viseur.html for more info). This program runs on SGI machines (IRIX 5.3,6.1,6.2). It is developped in collaboration with Sanofi Recherche Montpellier, a subdivision of the Elf Sanofi group. Sanofi decided to make the Viseur program available to academic researchers at no cost, according the program won't be used for commercial activities and any published work made with the Viseur program will acknowledge the authors as mentionned in the Viseur Copyright notice. So.. just request Viseur by email to obtain the latest release of the program if you are an academic user. I will send the release by email as a MIME encoded message by default. I'm about to leave the lab for one week (I'm going to the 24th Aharon Katzir-Katchalsky Conference: http://bioinformatics.weizmann.ac.il/conf/pdb25sw10/, some Viseur demo should take place there -)) but will try to reply to last week requests before leaving. best regards, Fabien Campagne -- campagne@incm.u-nancy.fr | Lab. de Chimie Theorique phone: (033) 83 91 20 00 extension 3236 | de Nancy, France. From owner-7tms_r@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!mr.net!www.nntp.primenet.com!nntp.primenet.com!mindspring!cssun.mathcs.emory.edu!news.service.emory.edu!news From: TJ Murphy Newsgroups: bionet.molbio.proteins.7tms_r Subject: Expression Cloning Date: Fri, 15 Nov 1996 17:22:14 -0500 Organization: Emory University Lines: 177 Message-ID: <328CED16.196D@bimcore.emory.edu> NNTP-Posting-Host: murphy1.pharm.emory.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Win95; I) Thanks to all who responded to my recent inquiry, you were very helpful. What follows is a list of citations compiled of all the GPCR's that have been cloned by functional expression in mammalian cells. Perhaps this will be helpful to somebody out there. Now, where is that fun and pleasure URL Tracy posted yesterday... T.J. Murphy 404-727-2467 Assistant Professor 404-727-0365 (fax) Emory University School of Medicine medtjm@bimcore.emory.edu Department of Pharmacology 1510 Clifton Road http://www.emory.edu/PHARMACOLOGY/MURPHY/ Atlanta, GA 30322 Abou-Samra, A. B., Juppner, H., Force, T., Freeman, M. W., Kong, X. F., Schipani, E., Urena, P., Richards, J., Bonventre, J. V., Potts, J. T., Jr., and a, e. (1992). Expression cloning of a common receptor for parathyroid hormone and parathyroid hormone-related peptide from rat osteoblast-like cells: a single receptor stimulates intracellular accumulation of both cAMP and inositol trisphosphates and increases intracellular free calcium. Proc.Natl.Acad.Sci.USA Proceedings of the National Academy of Sciences of the United States of America 89, 2732-2736. Birnbaumer, M., Seibold, A., Gilbert, S., Ishido, M., Barberis, C., Antaramian, A, Brabet, P., and Rosenthal, W. (1992). Molecular cloning of the receptor for human antidiuretic hormone. Nature 357, 333-335. Chen, R., Lewis, K. A., Perrin, M. H., and Vale, W. W. (1993). Expression cloning of a human corticotropin-releasing-factor receptor. Proc.Natl.Acad.Sci.USA Proceedings of the National Academy of Sciences of the United States of America 90, 8967-8971. Ebisawa, T., Karne, S., Lerner, M. R., and Reppert, S. M. (1994). Expression cloning of a high-affinity melatonin receptor from Xenopus dermal melanophores. Proc.Natl.Acad.Sci.USA Proceedings of the National Academy of Sciences of the United States of America 91, 6133-6137. Evans, C. J., Keith, D. E., Morrison, H., Magendzo, K., and Edwards, R. H. (1992). Cloning of a delta opiod receptor by functional expression. Science 258, 1952-1955. Gehlert, D. R., Beavers, L. S., Johnson, D., Gackenheimer, S. L., Schober, D. A., and Gadski, R. A. (1996). Expression cloning of a human brain neuropeptide Y Y2 receptor. Mol.Pharmacol. Molecular Pharmacology 49, 224-228. Gerald, C., Walker, M. W., Criscione, L., Gustafson, E. L., Batzl-Hartmann, C., Smith, K. E., Vaysse, P., Durkin, M. M., Laz, T. M., Linemeyer, D. L., Schaffhauser, A. O., Whitebread, S., Hofbauer, K. G., Taber, R. I., Branchek, T. A., and Weinshank, R. L. (1996). A receptor subtype involved in neuropeptide-Y-induced food intake [see comments]. Nature 382, 168-171. Gerald, C., Walker, M. W., Vaysse, P. J., He, C., Branchek, T. A., and Weinshank, R. L. (1995). Expression cloning and pharmacological characterization of a human hippocampal neuropeptide Y/peptide YY Y2 receptor subtype. J.Biol.Chem. Journal of Biological Chemistry 270, 26758-26761. Habert-Ortoli, E., Amiranoff, B., Loquet, I., Laburthe, M., and Mayaux, J.-F. (1994). Molecular cloning of a functional human galanin receptor. Proc. Natl. Acad. Sci. USA 91, 9780-9783. Hamann, J., Eichler, W., Hamann, D., Kerstens, H. M., Poddighe, P. J., Hoovers, J. M., Hartmann, E., Strauss, M., and van Lier, R. A. (1995). Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretion receptor superfamily with an unusual extracellular domain. J.Immunol. Journal of Immunology 155, 1942-1950. Hu, Y., Bloomquist, B. T., Cornfield, L. J., DeCarr, L. B., Flores-Riveros, J. R., Friedman, L., Jiang, P., Lewis-Higgins, L., Sadlowski, Y., Schaefer, J., Velazquez, N., and McCaleb, M. L. (1996). Identification of a novel hypothalamic neuropeptide Y receptor associated with feeding behavior. J. Biol. Chem. The Journal of Biological Chemistry 271, 26315-26319. Ishihara, T., Nakamura, S., Kaziro, Y., Takahashi, T., Takahashi, K., and Ngata, S. (1991). Molecular cloning and expression of a cDNA encoding the secretin receptor. EMBO J. The EMBO Journal 10, 1635-1641. Jelinek, L. J., Lok, S., Rosenberg, G. B., Smith, R. A., Grant, F. J., Biggs, S., Bensch, PA, Kuijper, J. L., Sheppard, P. O., Sprecher, C. A., and a, e. (1993). Expression cloning and signaling properties of the rat glucagon receptor. Science 259, 1614-1616. Juppner, H., Abou-Samra, A.-B., Freeman, M., Kong, X. F., Schipani, E., Richards, J., Kolakowski, L. F., Hock, J., Potts, J. T., Kronenberg, H. M., and Segre, G. V. (1991). A G protein-linked receptor for parathyroid hormone and parathyroid hormone-related peptide. Science 254, 1024-1026. Kambayashi, Y., Bardhan, S., Takahashi, K., Tsuzuki, S., Inui, H., Hamakubo, T., and Inagami, T. (1993). Molecular cloning of a novel angiotensin II receptor isoform involved in phosphotyrosine phosphatase inhibition. J.Biol.Chem. The Journal of Biological Chemistry 268, 24543-24546. Kieffer, B. L., Befort, K., Gaveriaux-Ruff, C., and Hirth, C. G. (1992). The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization [published erratum appears in Proc Natl Acad Sci U S A 1994 Feb 1;91(3):1193]. Proc.Natl.Acad.Sci.USA Proceedings of the National Academy of Sciences of the United States of America 89, 12048-12052. Kluxen, F. W., Bruns, C., and Lubbert, H. (1992). Expression cloning of a rat brain somatostatin receptor cDNA. Proc.Natl.Acad.Sci.USA Proceedings of the National Academy of Sciences of the United States of America 89, 4618-4622. Kopin, A. S., Lee, Y. M., McBride, E. W., Miller, L. J., Lu, M., Lin, H. Y., Kolakowski, L. F., Jr, and Beinborn, M. (1992). Expression cloning and characterization of the canine parietal cell gastrin receptor. Proc.Natl.Acad.Sci.USA Proceedings of the National Academy of Sciences of the United States of America 89, 3605-3609. Lin, H. Y., Harris, T. L., Flannery, M. S., Aruffo, A., Kaji, E. H., Gorn, A., Kolakowski, L. F., Lodish, H. F., and Goldring, S. R. (1991). Expression cloning of an adenylate cyclase-coupled calcitonin receptor. Science 254, 1022-1024. Lin, H. Y., Kaji, E. H., Winkel, G. K., Ives, H. E., and Lodish, H. F. (1991). Cloning and functional expression of a vascular smooth muscle endothelin 1 receptor. Proc. Natl. Acad. Sci. USA 88, 3185-3189. Mukoyama, M., Nakajima, M., Horiuchi, M., Sasamura, H., Pratt, R. E., and Dzau, V. J. (1993). Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors. J.Biol.Chem. The Journal of Biological Chemistry 268, 24539-24542. Murphy, T. J., Alexander, R. W., Griendling, K. K., Runge, M. S., and Bernstein, K. E. (1991). Isolation of a cDNA encoding the vascular type-1 angiotensin ll receptor. Nature 351, 233-236. Reagan, J. D. (1994). Expression cloning of an insect diuretic hormone receptor. A member of the calcitonin/secretin receptor family. J.Biol.Chem. Journal of Biological Chemistry 269, 9-12. Sakurai, T., Yanagisawa, M., Takuwa, Y., Miyazaki, H., Kimura, S., Goto, K., and Masaki, T. (1991). Cloning of a cDNA encoding a non-isopeptide-selective subtype of the endothelin receptor. Nature 348, 732-735,. Sasaki, K., Yamano, Y., Bardhan, S., Iwai, N., Murray, J. J., Hasegawa, M., Matsuda, Y., and Inagami, T. (1991). Cloning and expression of a complementary DNA encoding a bovine adrenal angiotensin II type-1 receptor. Nature 351, 230-232. Spengler, D., Waeber, C., Pantaloni, C., Holsboer, F., Bockaert, J., Seeburg, PH, and Journot, L. (1993). Differential signal transduction by five splice variants of the PACAP receptor. Nature 365, 170-175. Thorens, B. (1992). Expression cloning of the pancreatic beta cell receptor for the gluco-incretin hormone glucagon-like peptide 1. Proc.Natl.Acad.Sci.USA Proceedings of the National Academy of Sciences of the United States of America 89, 8641-8645. Xie, G. X., Miyajima, A., and Goldstein, A. (1992). Expression cloning of cDNA encoding a seven-helix receptor from human placenta with affinity for opioid ligands [published erratum appears in Proc Natl Acad Sci U S A 1992 Aug 1;89(15): 7287]. Proc.Natl.Acad.Sci.USA Proceedings of the National Academy of Sciences of the United States of America 89, 4124-4128. -- T.J. Murphy 404-727-2467 Assistant Professor 404-727-0365 (fax) Emory University School of Medicine medtjm@bimcore.emory.edu Department of Pharmacology http://www.emory.edu/PHARMACOLOGY/MURPHY/ 1510 Clifton Road Atlanta, GA 30322 From owner-7tms_r@net.bio.net Thu Nov 14 22:00:00 1996 Path: biosci!FARMR1.MED.UTH.TMC.EDU!aschonb From: aschonb@FARMR1.MED.UTH.TMC.EDU (A. Schonbrunn) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoctoral Position Date: 15 Nov 1996 15:33:46 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Postdoctoral positions in G protein coupled receptors University of Texas. Two postdoctoral positions are available to work on somatostatin receptors in the laboratory of Agi Schonbrunn in the Dept of Integrative Biology, Pharmacology and Physiology. Research areas include examination of the functional importance of receptor phosphorylation in desensitization and receptor internalization, specificity of receptor-G protein coupling and the development of in vitro and transgenic model systems to examine molecular mechanisms of somatostatin induced apoptosis. Candidates should have a recent PhD in an appropriate field and experience in one or more of the following: protein biochemistry, site-directed mutagenesis, eukaryotic protein expression, signal transduction methods, apoptosis. Applicants should send a curriculum vitae, statement of research experience and career objectives and the names of three references to Dr. Schonbrunn at the address below. A. Schonbrunn, Ph.D. Professor Dept of Integrative Biology, Pharmacology & Physiology Univ. of Texas Medical School - Houston P.O.Box 20708, Houston,TX 77225 e-mail: aschonb@farmr1.med.uth.tmc.edu FAX: 713-792-5985 ************************************************************************** Agnes Schonbrunn, Ph.D. e-mail: aschonb@farmr1.med.uth.tmc.edu Professor FAX: 713-792-5985 Dept of Integrative Biology, Pharmacology & Physiology Univ. of Texas Medical School P.O.Box 20708, Houston,TX 77225 From owner-7tms_r@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!howland.erols.net!newsfeed.internetmci.com!hunter.premier.net!hammer.uoregon.edu!arclight.uoregon.edu!usenet.eel.ufl.edu!warwick!lyra.csx.cam.ac.uk!daresbury!not-for-mail From: "Anja Garritsen" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Adenylyl cyclase Date: 18 Nov 1996 15:49:01 -0000 Lines: 7 Sender: lpddist@mserv1.dl.ac.uk Distribution: bionet Message-ID: <56q0hd$o94@mserv1.dl.ac.uk> App-Message-ID: <8151321618111996/A08265/MIMAS/11AB94203300> Sensitivity: Company-Confidential Original-To: "7TM" <7tms_r@dl.ac.uk> After endogenous receptors and G proteins, the effector enzymes! Who knows which adenylyl cyclase isoforms are expressed in CHO cells and other cell lines used for transfections (3T3, Ltk-, HEK-293 etc). We see pronounced differences in the stimulation by forskolin and receptor ligands. Anja Garritsen From owner-7tms_r@net.bio.net Sun Nov 17 22:00:00 1996 Path: biosci!news.ohsu.edu!NewsWatcher!user From: forte@ohsu.edu (Michael Forte) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Co-Receptors Date: 18 Nov 1996 19:56:29 GMT Organization: Vollum Institute Lines: 11 Message-ID: NNTP-Posting-Host: 137.53.73.42 I was wonder if anyone had information (references etc) on the existence of what I will call co-receptors, i.e., other proteins, particularly transmembrane proteins, which are required for the activity or function of a GPCR. Mike Forte -- Mike Forte forte@ohsu.edu or forte@mtwo.com From owner-7tms_r@net.bio.net Mon Nov 18 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!news.sgi.com!iag.net!www.nntp.primenet.com!nntp.primenet.com!feed1.news.erols.com!howland.erols.net!EU.net!usenet2.news.uk.psi.net!uknet!usenet1.news.uk.psi.net!uknet!dispatch.news.demon.net!demon!ferring.demon.co.uk From: Steve Qi Newsgroups: bionet.molbio.proteins.7tms_r Subject: Need help in expression of GnRH receptor in insect cells Date: Tue, 19 Nov 1996 14:10:17 +0000 Organization: Ferring Research Institute Lines: 12 Message-ID: <3291BFC9.4359@ferring.demon.co.uk> Reply-To: sqi@ferring.demon.co.uk NNTP-Posting-Host: ferring.demon.co.uk X-NNTP-Posting-Host: ferring.demon.co.uk X-Mailer: Mozilla 2.02 (Macintosh; I; 68K) MIME-Version: 1.0 CC: sqi@ferring.demon.co.uk Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit Dear readers. I would be very grateful if you can help me in expression the human GnRH receptor in the insect cell system. The gene for the receptor has been cloned and inorder to produce large amount of receptors, we would like to express it in the baculovirus. If you know anybody has done this succesfully, please send me the information. Yours faithfully, Steve Qi sqi@ferring.demon.co.uk From owner-7tms_r@net.bio.net Tue Nov 19 22:00:00 1996 Path: biosci!ihnp4.ucsd.edu!swrinde!cs.utexas.edu!www.nntp.primenet.com!nntp.primenet.com!su-news-hub1.bbnplanet.com!news.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cpk-news-feed4.bbnplanet.com!maze.dpo.uab.edu!usenet From: Wayne Duck Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Need help in expression of GnRH receptor in insect cells Date: Wed, 20 Nov 1996 08:48:25 -0600 Organization: UAB Lines: 20 Message-ID: <32931A39.45FA@phy164.physiology.uab.edu> References: <3291BFC9.4359@ferring.demon.co.uk> Reply-To: duck@phy164.physiology.uab.edu NNTP-Posting-Host: phy164.physiology.uab.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I) Steve Qi wrote: > > Dear readers. > > I would be very grateful if you can help me in expression the human GnRH > receptor in the insect cell system. The gene for the receptor has been > cloned and inorder to produce large amount of receptors, we would like > to express it in the baculovirus. If you know anybody has done this > succesfully, please send me the information. Neill, J.D., J.C. Sellers, and L.W. Duck. Expression of the gonadotropin releasing hormone (GnRH) receptor in the baculovirus/insect cell (Sf9) system. Society for Neuroscience Annual Meeting, San Diego, CA, November, 11-16, 1995 (Abstract #402.16). -- Wayne Duck http://www.physiology.uab.edu/neill/wayne/ Dept. of Physiology and Biophysics University of Alabama at Birmingham From owner-7tms_r@net.bio.net Fri Nov 22 22:00:00 1996 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!news.mathworks.com!uunet!in1.uu.net!wilbur.ohww.norman.ok.us!news.msfc.nasa.gov!xmission!mail.nemonet.com!sbolting From: sbolting@nemonet.com (Stephen Boltinghouse) Subject: Just try this, it will work Newsgroups: alt.sex.prostitution,alt.sex.prostitution.tijuana,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,bionet.molbio.proteins.fluorescent,comp.sys.proteon,de.comm.protocols.tcp-ip,de.comm.protocols.zconnect,dfw.internet.providers,fj.net.providers,houston.internet.providers,ger.ct.pruefstand,fj.soc.pseudo-science,bionet.organisms.pseudomonas,rainbow.mailing-lists.psfilt,alt.paranet.psi,comp.sys.psion.apps,comp.sys.psion.marketplace,comp.sys.psion.misc,comp.sys.psion.programmer,alt.support.psoriasis,alt.fan.psyberhut,uw.psychology.psyc207,cu.courses.psyc4145,uw.psychology.psyc463a,uw.psychology.psyc647a,alt.music.psychedelic,alt.personals.psychedelic,alt.rock-n-roll.psychedelic,rec.drugs.psychedelic,alt.drugs.psychedelics,alt.flame.psychiatry,sci.med.psychobiology,pl.sci.psychologia,de.sci.psychologie,fj.sci.psychology,sci.psychology.psychotherapy,alt.irc.psycloud,sfnet.keskustelu.psykologia,comp.soft-sys.ptolemy,fj.org.ptt,alt.christnet.public,alt.tv.public-access,alt.fan.publius Date: Sat, 23 Nov 1996 13:47:03 GMT Message-ID: <358.896244412754@news.nemonet.com> NNTP-Posting-Host: 205.138.176.47 X-Newsreader: News Xpress Version 1.0 Beta #4 Organization: The fastest way towards earning money honestly Lines: 142 Xref: biosci bionet.molbio.proteins:9402 bionet.molbio.proteins.7tms_r:939 bionet.molbio.proteins.fluorescent:844 comp.sys.proteon:192 bionet.organisms.pseudomonas:187 comp.sys.psion.apps:1656 comp.sys.psion.marketplace:781 comp.sys.psion.misc:4157 comp.sys.psion.programmer:1075 rec.drugs.psychedelic:21501 sci.med.psychobiology:13840 sci.psychology.psychotherapy:18801 comp.soft-sys.ptolemy:2136 Take five minutes to read this and it WILL change your life. The Internet has grown tremendously. It doubles in size every 4 months. think about it. You see those 'Make.Money.Fast' posts more and more. That's ... because it WORKS ! So I thought, all those new users might make it work. And I decided to try it out, a few months ago. Besides, whats $5.00, I spend more than that in the morning on my way to work on coffee and cigs for the day. So I sent in my money and posted. Everyone was calling it a scam, but there are SO many new users from AOL, Netcom, etc. they will join in and make it work for you. Well, two weeks later, I began recieving bucks in the mail! I couldn't believe it! Not just a little, I mean big bucks! At first only a few hundred dollars, then a week later, a couple of thousand, then BOOM. By the end of the fourth week, I had recieved nearly $47,000.00. It came from all over the world. And every bit of it perfectly legal and on the up and up. I've been able to pay off all my bills and still had enough left over for a nice vacation for me and my family. Not only does it work for me, it works for other folks as well. Markus Valppu says he made $57,883 in four weeks. Dave Manning claims he made $53,664 in the same amount of time. Dan Shepstone says it was only $17,000 for him. Do I know these folks? No, but when I read how they say they did it, it made sense to me. Enough sense that I'm taking a similar chance with $5 of my own bucks. Not a big chance, I admit--but one with incredible potential, because $5 is all anyone ever invests in this system. Period. That's all Markus, Dave, or Dan invested, yet their $5 netted them tens of thousands of dollars each, in a safe, legal, completely legitimate way. Here's how it works in 3 easy steps: STEP 1. Invest your $5 by writing your name and address on five seperate pieces of paper along with the words: "PLEASE ADD ME TO YOUR MAILING LIST." (In this way, you're not just sending a dollar to someone; you're paying for a legitimate service.) Fold a $1 bill, money order, or bank note inside each paper, and mail them by standard U. S. Mail to the following five addresses: 1- Fern Suarez Mallorca 112 Hato Rey, P.R., USA, 00917 2- Philippe 2104 De Mexico Chomedey, Laval Quebec, Canada H7M 3C6 3- Natalie Jansen Lancveldlaan 18 5671 CN Nuenen Holland 4- Chad Collier 2785 Cold Springs Rd. #49 Placerville, CA 95667 5- Steve Boltinghouse 1009 Bird St. Hannibal, MO 63401 STEP 2. Now remove the top name from the list, and move the other names up.This way, #5 becomes #4 and so on. Put your name in as the fifth one on the list. STEP 3. Post the article to at least 250 newsgroups. There are at least 19000 newsgroups at any given moment in time. Try posting to as many newsgroups as you can. Remember the more groups you post to, the more people will see your article and send you cash! STEP 4. 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Finally, 5,000 people make 250 postings each with your name at #1 and you get a return of $60,000 before your name drops off the list.And that's only if everyone down the line makes only 250 postings each! Your total income for this one cycle is $55,000. From time to time when you see your name is no longer on the list, you take the latest posting you can find and start all over again. The end result depends on you. You must follow through and repost this article everywhere you can think of. The more postings you make, the more cash ends up in your mailbox. It's too easy and too cheap to pass up!!! So thats it. Pretty simple sounding stuff, huh? But believe me, it works. There are millions of people surfing the net every day, all day, all over the world. And 100,000 new people get on the net every day. You know that, you've seen the stories in the paper. So, my friend, read and follow the simple instructions and play fair. Thats the key, and thats all there is to it. Print this out right now so you can refer back to this article easily. Try to keep an eye on all the postings you made to make sure everyone is playing fairly. You know where your name should be. If you're really not sure or still think this can't be for real, then don't do it. But please print this article and pass it along to someone you know who really needs the bucks, and see what happens. REMEMBER....HONESTY IS THE BEST POLICY.YOU DON'T NEED TO CHEAT THE BASIC IDEA TO MAKE THE BUCKS! GOOD LUCK TO ALL, AND PLEASE PLAY FAIR AND YOU WILL WIN AND MAKE SOME REAL INSTANT FREE CASH! *** By the way, if you try to deceive people by posting the messages with your name in the list and not sending the bucks to the people already included, you will not get much. I know someone who did this and only got about $150 (and that's after two months). Then he sent the 5 bills, people added him to their lists, and in 4-5 weeks he had over $10,000! TRY IT AND YOU'LL BE HAPPY!!! :o) !!!!!!!!!! From owner-7tms_r@net.bio.net Sun Nov 24 22:00:00 1996 Path: biosci!agate!howland.erols.net!news-peer.gsl.net!news.gsl.net!news-stkh.gsl.net!news.gsl.net!nntp-oslo.UNINETT.no!nntp-trd.UNINETT.no!news.uit.no!atf1!edvard From: edvard@atf1.imb.fm.uit.no (Oyvind Edvardsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: GRAP mutant database Date: 25 Nov 1996 14:12:10 GMT Organization: University of Tromsoe Lines: 73 Distribution: bionet Message-ID: <57c9fq$n2o@news.uit.no> Reply-To: edvard@atf1.imb.fm.uit.no NNTP-Posting-Host: atf1.imb.fm.uit.no We are pleased to announce the availability of the mutant catalog tinyGRAP. We have stripped the effect data in GRAP and constructed a simple mutant catalog which is much more updated than GRAP. This searchable mutant catalog, tinyGRAP, has information on the receptor, literature reference, substitution(s) and the domain(s) in which the substitution(s) was (were) made. After reformatting the GRAP data we have added ~1000 new mutants to the catalog, bringing the grand total up to more than 3000 mutants published in ~500 papers. Thus, we have added information from almost 200 papers. Furthermore, tinyGRAP users have access to references to ~200 additional papers that describe deletion, insertion and/or chimeric mutant receptors. Such mutants have NOT been included in the mutant database, only substitution mutants. However, through the literature reference search facility, the references to these papers can be obtained. The format of the data has been changed completely, but the search facilities are virtually the same as in GRAP. There are links into the SwissProt and GCRDb sequence databases, and we have also included links to the original GRAP, EntrezMEDLINE and OMIM. For substitutions in the TM regions, tinyGRAP attempts to calculate the position identifier e.g. (3.48) [vanRhee & Jacobson, (1996) Drug Dev.Res. 37,1-38]. The calculated position identifiers are currently very unreliable, since they are being calculated based on the amino acid sequence of the single receptor and the TM amino acid signature. We are also pleased to announce the GRAP logo which will be visible on every page retrieved from GRAP and tinyGRAP. The logo was designed by myself and Roy A. Lysaa at the Dept. of Pharmacology, Univ. of Tromsoe. Many thanks are due to RAL for his graphics skills and kind cooperation. Release history of tinyGRAP: 1.0 - Data from GRAP converted to the tinyGRAP format. 2.0 - The majority of the new data was added. 2.1 \ 2.2 Versions used for internal testing and a number of corrections were made. 2.3 / 3.0 - Another ~80 mutants were added. 3.1 - Some minor corrections to mutants and literature references. Thus, release 3.1 is the first public release of tinyGRAP. We would like to thank Dr. G. Vriend and Dr. M. Beukers for many useful comments and interesting discussions. In order to be able to continue to provide computerized mutant data we need YOUR help. If you can't locate YOUR paper(s) in tinyGRAP, at least send us a notification. Even better if YOU would send reprint(s). The postal address is given below. Access tinyGRAP from the GRAP homepage: http://www-grap.fagmed.uit.no/GRAP/homepage.html OEyvind. -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Sun Nov 24 22:00:00 1996 Path: biosci!news.ohsu.edu!NewsWatcher!user From: forte@ohsu.edu (Michael Forte) Newsgroups: bionet.molbio.proteins.7tms_r Subject: GPCR "Co-Receptors" Date: 25 Nov 1996 19:57:59 GMT Organization: Vollum Institute Lines: 23 Message-ID: NNTP-Posting-Host: 137.53.73.42 Hi Folks, Recently, I posted a query here about any info concerning proteins, particulary transmembrane proteins, which are required for the function of GPCR. I received lots of responses, none with information but all asking to be informed of what I found out. So, in response to all those who were interested, there really is no information, to my knowledge, on proteins which function as "co-receptors" for any GPCR. The exception to this would be a protein which is apparently required for CGRP responsiveness but this protein is largely hydrophilic (PNAS 93:3455). The only other example is the emerging story of patched and smoothened. Patched (ptc), a 12 TM protein, acts as the receptor for the morphogen hedgehog (hh). In the absence of ligand, ptc acts to inhibit the activity of smoothened (smo) which appears to be a 7 TM receptor of the GPRC class. Inhibition of smo by ptc is apparently overcome by binding of hh to ptc. Of course, any other info would be appreciated. Mike Forte -- Mike Forte forte@ohsu.edu or forte@mtwo.com From owner-7tms_r@net.bio.net Thu Nov 28 22:00:00 1996 Path: biosci!FERRE.SACLAY.CEA.FR!Nicolas From: Nicolas@FERRE.SACLAY.CEA.FR (Nicolas Ancellin) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 29 Nov 1996 08:21:33 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 3 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe