From owner-7tms_r@net.bio.net Sat Mar 01 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!newspump.sol.net!mindspring!news3.agis.net!agis!newsgod1.agis.net!agis!newspeer1.agis.net!agis!news.mwci.net!not-for-mail From: lazyhog@mwci.net (Chris R. Hoffman) Newsgroups: bionet.cellbiol,bionet.biology.cardiovascular,bionet.microbiology,sci.bio.misc,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,bionet.molbio.proteins.fluorescent Subject: TUBULIN USERS PLEASE READ Date: 3 Mar 1997 02:43:51 GMT Organization: Lost Creek Swine Lines: 10 Message-ID: <5fddt7$b71$1@hihat.mwci.net> NNTP-Posting-Host: dbq-dial-3.dbq.mwci.net Mime-Version: 1.0 X-Newsreader: WinVN 0.99.2 Xref: biosci bionet.cellbiol:6815 bionet.biology.cardiovascular:1540 bionet.microbiology:9159 sci.bio.misc:7566 bionet.molbio.proteins:10141 bionet.molbio.proteins.7tms_r:1060 bionet.molbio.proteins.fluorescent:980 Attention all Tubulin buyers Check out microSuppliers new web page on the internet Special pricing for NEW CUSTOMERS!!!! Go to http://users.mwci.net/~microsup CHECK US OUT TODAY From owner-7tms_r@net.bio.net Mon Mar 03 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!news.sgi.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!EU.net!Norway.EU.net!uninett.no!news.uit.no!news From: edvard@fagmed.uit.no (Oyvind Edvardsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: SUMMARY: GRAP usage Date: 5 Mar 1997 07:40:23 GMT Organization: Univ. of Tromso, IMB Lines: 36 Message-ID: <5fj817$mpu@news.uit.no> Reply-To: edvard@fagmed.uit.no NNTP-Posting-Host: fa13.imb.fm.uit.no Recently I posted the questions: 1) In what way is the GRAP databases useful to you? 2) For what purpose(s) do you use the GRAP databases? Although there is not so much to sum up I have prepared a sort of summary of what has been received. The responses were: a) Look around. b) Keep track of published mutant data for particular type(s) of receptor(s), or keeping track of mutant data for GPCRs in general. c) Get ligand binding data on single point mutants. d) Use data for receptor modelling and validation. There were various comments on the data content of the databases. Thanks to the enormous amount of FOUR persons for giving their responses to my questions ;-)) !!! OEyvind. -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Mon Mar 03 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!news-xfer.netaxs.com!feed1.news.erols.com!howland.erols.net!news.nacamar.de!newsfeed.nacamar.de!supernews.com!news From: barmaster Newsgroups: bionet.molbio.proteins.7tms_r Subject: teach clark Date: Tue, 04 Mar 1997 23:48:27 -0800 Organization: All USENET -- http://www.SuperNews.com Lines: 1 Message-ID: <331D254B.79E7@aros.net> NNTP-Posting-Host: 207.173.24.22 Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.01 (Win95; I; 16bit) ignore this it is a test. From owner-7tms_r@net.bio.net Mon Mar 03 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!news.sgi.com!mr.net!news.mr.net!urvile.msus.edu!news From: wooinc (wooinc) Newsgroups: bionet.molbio.proteins.7tms_r Subject: I like to have sex Date: 4 Mar 1997 20:08:09 GMT Organization: hello Lines: 2 Message-ID: <5fhvf9$o73@Urvile.MSUS.EDU> NNTP-Posting-Host: 198.174.74.119 Mime-Version: 1.0 Content-Type: Text/Plain; charset=ISO-8859-1 X-Newsreader: WinVN 0.99.5 Sex dude From owner-7tms_r@net.bio.net Tue Mar 04 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!europa.clark.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!EU.net!Norway.EU.net!uninett.no!news.uit.no!news From: edvard@fagmed.uit.no (Oyvind Edvardsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: ANNOUNCE: tinyGRAP release 4.0 Date: 5 Mar 1997 13:07:17 GMT Organization: Univ. of Tromso, IMB Lines: 38 Message-ID: <5fjr65$qnv@news.uit.no> Reply-To: edvard@fagmed.uit.no NNTP-Posting-Host: fa13.imb.fm.uit.no tinyGRAP release 4.0 is immediately available for consumption. We have added around 400 mutants bringing the grand total number of mutants over the 3500 mark. A total number of around 770 mutant papers is available. We have added the keywords chimeric, deletion and insertion to the appropriate papers in the database such that papers dealing with such mutants will be more readily available using the literature reference search facility. We have added two new items on the query form: 1) Do mutant searches only in the current database release. 2) Add titles to the list of papers returned from a literature reference search (this has been present for a while but is now 'official'). We have shortened the major heading on each page to read "GPCRs, Family A". We have also reorganized the GRAP database homepage somewhat. The contents remain ~ the same as before. The official and non-volatile entry point into the GRAP databases is still http://www-grap.fagmed.uit.no/GRAP/homepage.html. OEyvind. -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Wed Mar 05 22:00:00 1997 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: moderation Date: 6 Mar 1997 07:48:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 20 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703061548.QAA20516@nu> NNTP-Posting-Host: net.bio.net A few weeks ago I told you I would investigate some of those stupid messages send to us about available porno pictures etc. The last four weeks I have investigated the origin of every such message. The score is that one stolen account got closed in Kroatia, and three users got expelled by their internet provider. Two messages where hidden too cleverly to find out where they came from, and one provider (earthlink.net) refuses to respond to my questions. As the amount of work to chase those email culprits is not overwhelming, I will keep doing it for a while. So, just delete the messages that obviously should never have been sent, knowing that there is about 50% chance that the sender will be punished. Alternatively, you can move from the mailing list to using the news group. Gert Vriend (Ps. If you want to respond, respond to me, and not to the list. If relevant topics are brought up, I will post a summary of the reactions). Gert From owner-7tms_r@net.bio.net Tue Mar 11 22:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Mar 1997 02:00:13 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 239 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703121000.CAA13274@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. Sincerely, Dave Kristofferson BIOSCI/bionet Manager biosci-help@net.bio.net From owner-7tms_r@net.bio.net Thu Mar 13 22:00:00 1997 Path: biosci!news.ohsu.edu!NewsWatcher!user From: forte@ohsu.edu (Michael Forte) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: ppting small amnts protein Date: 14 Mar 1997 23:58:52 GMT Organization: OHSU Lines: 22 Message-ID: References: <5gcjum$gpm@cronkite.ocis.temple.edu> NNTP-Posting-Host: 137.53.98.58 We have always used TCA, which works fine if your protein is not acid-labile. So, add TCA to 5 or 10%, incubate on ice for 15 min., spin in a microfuge for 15 min., either wash the pellet with ice cold acetone or resuspend in SDS sample buffer with bromphenyl blue. Without the acetone step, the bromophenyl blue will turn green. So, before loading, I usually add a ul or 2 or 1 NaOH untile it turns blue again. Heat and load. Mike Forte In article <5gcjum$gpm@cronkite.ocis.temple.edu>, waring@astro.ocis.temple.edu (Richard Waring) wrote: > I am familiar with precipitating small amounts of nucleic acids > using carrier. What would be suitable for precipitating small > amounts of protein that do not come down with acetone. We > need to concentrate samples to load them on a SDS gel. > Thanks > > > -- > Best wishes > Richard Waring From owner-7tms_r@net.bio.net Thu Mar 13 22:00:00 1997 Path: biosci!IX.NETCOM.COM!jonescox From: jonescox@IX.NETCOM.COM (Rich Cox) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Nonradioactive methods of receptor binding Date: 14 Mar 1997 15:09:15 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 10 Sender: daemon@net.bio.net Distribution: world Message-ID: <3329DB62.4E18@ix.netcom.com> NNTP-Posting-Host: net.bio.net We are becoming increasingly interested in utilizing non-radioactive methods for detection of receptor-ligand interactions. The overall goal is to reduce radioligand usage. We are familiar with a few: eg, time-resolved fluorescence, homogeneous time-resolved fluorescence, fluoresence polarization, and reporter systems. If anyone is familiar with or has experience with other methods please post. We are not restricted to fluorescence. Thanks, Rich Cox From owner-7tms_r@net.bio.net Thu Mar 13 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!cs.utexas.edu!news.sprintlink.net!news-peer.sprintlink.net!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news-xfer.netaxs.com!cronkite.ocis.temple.edu!astro.ocis.temple.edu!waring From: waring@astro.ocis.temple.edu (Richard Waring) Newsgroups: bionet.molbio.proteins.7tms_r Subject: ppting small amnts protein Date: 14 Mar 1997 22:37:10 GMT Organization: Temple University, Academic Computer Services Lines: 10 Message-ID: <5gcjum$gpm@cronkite.ocis.temple.edu> NNTP-Posting-Host: astro.ocis.temple.edu X-Newsreader: TIN [version 1.2 PL2] I am familiar with precipitating small amounts of nucleic acids using carrier. What would be suitable for precipitating small amounts of protein that do not come down with acetone. We need to concentrate samples to load them on a SDS gel. Thanks -- Best wishes Richard Waring From owner-7tms_r@net.bio.net Sun Mar 16 22:00:00 1997 Newsgroups: bionet.molbio.proteins.7tms_r Path: biosci!agate!howland.erols.net!rill.news.pipex.net!pipex!warwick!bris.ac.uk!usenet From: Andrew Doherty Subject: Re: ppting small amnts protein X-Nntp-Posting-Host: anaajd.ana.bris.ac.uk Content-Type: text/plain; charset=us-ascii To: Richard Waring Message-ID: <332D05C2.5FD0@Bris.ac.uk> Sender: usenet@fsa.bris.ac.uk (Usenet) Content-Transfer-Encoding: 7bit Organization: University of Bristol, UK References: <5gcjum$gpm@cronkite.ocis.temple.edu> Mime-Version: 1.0 Date: Mon, 17 Mar 1997 08:50:10 GMT X-Mailer: Mozilla 3.0 (Win95; I) Lines: 38 Richard Waring wrote: > > I am familiar with precipitating small amounts of nucleic acids > using carrier. What would be suitable for precipitating small > amounts of protein that do not come down with acetone. We > need to concentrate samples to load them on a SDS gel. > Thanks > > -- > Best wishes > Richard Waring I always use 3 vol MeOH, 1 vol CHCl3 and 4 vol water (mixing between each addition), spin in a microfuge for 60 seconds or so, remove the top layer (protein is at the interface), add a further 3 vol MeOH, spin for 2 mins and air dry your protein pellet. It's the method of Wessel & Flugge (1984) Anal. Biochem. 138; pp 141-143. They say you can precipitate down to 40 micro g protein quantitatively from all sorts of solutions including detergents. I've precipitated down to 20-30 microgrames using this technique for westerns and it works fine. I've also recovered small amounts of protein (say 50-100 micrograms) from up to 1ml of solution. Just remember to mix well between each addition in the initial stages, and that your methanol and chlorofom are of the highest quality you can get. Hope it helps Andy D -- ************************************************************* Dr Andrew Doherty email - a.doherty@bris.ac.uk Dept. Anatomy Tel (0117)9287421 School of Medical Sciences Fax (0117)9287402 University of Bristol University Walk Bristol UK BS8 1TD ************************************************************* From owner-7tms_r@net.bio.net Sun Mar 16 22:00:00 1997 Path: biosci!uni-duesseldorf.de!schuessl From: schuessl@uni-duesseldorf.de (Peter Schuessler) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: ppting small amnts protein Date: 17 Mar 1997 01:41:18 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 41 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703170941.BAA11988@net.bio.net> NNTP-Posting-Host: net.bio.net Dear Richard, in our lab, we have compared several methods for precipitating small amounts of protein. We achieved the best results with methanol and chloroform. You add 4 volumes methanol and 1 volume chloroform to your probe, than add 3 volumes water. Spin for 30 min at 4=B0C and 15,000g. Discard the supernatant carefully, the proteins are in a white interphase. Add 3 volumes methanol and mix. Spin again. Discard the supernatant, dry the pellet on air and resolve the proteins with SDS sample buffer. Good luke, Peter >I am familiar with precipitating small amounts of nucleic acids=20 >using carrier. What would be suitable for precipitating small=20 >amounts of protein that do not come down with acetone. We=20 >need to concentrate samples to load them on a SDS gel. >Thanks=20 > > >-- >Best wishes >Richard Waring > > > --------------------------------------------------------- | Peter Schuessler | | Genetic Parasitology |=20 | Institute for Genetics | | Heinrich-Heine-University Duesseldorf | | Universitaetsstr.1 | | D-40225 Duesseldorf | | Germany | | | | Tel: ++49-(0)211-81-12408 | | Fax: ++49-(0)211-81-12333 | | e-mail: schuessl@mail.rz.uni-duesseldorf.de | | http://www.uni-duesseldorf.de/WWW/MathNat/Parasitology/ | --------------------------------------------------------- From owner-7tms_r@net.bio.net Thu Mar 20 22:00:00 1997 Path: biosci!pharm.som.sunysb.edu!meiling From: meiling@pharm.som.sunysb.edu (Meiling Shih) Newsgroups: bionet.molbio.proteins.7tms_r Subject: multiple ligand binding sites Date: 21 Mar 1997 08:25:21 -0800 Organization: Pharmacology - SUNY Stony Brook Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <3332B592.737B@pharm.som.sunysb.edu> NNTP-Posting-Host: net.bio.net Does anyone know if there are programs to analyze receptor binding that is more than two sites? Any wild-type or mutant G protein-linked receptors have been fit to 3-4 affinity sites in the literature? All suggetions are appreciated. Meiling Shih, Ph. D. Dept. of Pharmacology SUNY/Stony Brook phone 516-4447873 E-mail: meiling@pharm.som.sunysb.edu From owner-7tms_r@net.bio.net Thu Mar 20 22:00:00 1997 Path: biosci!YALE.EDU!henrik.dohlman From: henrik.dohlman@YALE.EDU (Henrik Dohlman) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (none) Date: 21 Mar 1997 10:32:02 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net subscribe 7tms_r end Henrik G. Dohlman, PhD Assistant Professor Department of Pharmacology Yale University School of Medicine Room 436 BCMM, P.O. Box 9812 295 Congress Ave. New Haven, CT 06536 203 737 2203 203 737 2290 FAX henrik.dohlman@yale.edu http://info.med.yale.edu/bcmm/dohlman/DohlmanLab.html From owner-7tms_r@net.bio.net Fri Mar 21 22:00:00 1997 Path: biosci!SUN5.IBP.AC.CN!huang From: huang@SUN5.IBP.AC.CN ("Huang Hu") Newsgroups: bionet.molbio.proteins.7tms_r Subject: 97 China Symposium of Young Scientists on Frontier of Biological Membrane Research Date: 22 Mar 1997 05:48:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 148 Sender: daemon@net.bio.net Distribution: world Message-ID: <9703221242.AA03885@sun5> NNTP-Posting-Host: net.bio.net Please help me to post this announcement to other sites. Thank you. ---------------------------------------------------------------------------- -- `97 China Symposium of Young Scientists on Frontier of Biological Membrane Research ---------------------------------------------------------------------------- -- Sponsored by : the Chinese Academy of Sciences National Natural Science Foundation Venue: National Laboratory of Biomacromolecules Institute of Biophysics, Academia Sinica Period: Dec. 20-25, 1997 First Announcement Call for Titles of the Article, Abstracts, Curriculum Vitae and Registration ---------------------------------------------------------------------------- -- 1. PURPOSE: To promote the contact and the academic exchange among young Chinese scholars home and abroad To explore the possibilities of cooperation in the membrane research field 2. CONTENTS Results, proceedings and reviews on the structure and function of biological membrane Current advances and perspectives of the biological membrane research TOPICS (1) Transmembrane signal transduction (2) Membrane lipid- protein interactions (3) Protein translocation (4) Structure of membrane protein (5) F,V and P type ATPases (6) Apoptosis and membrane (7) Advanced techniques and approaches in biological membrane research 3. PARTICIPANTS AND REQUIREMENTS PARTICIPANTS: Chinese scholars home and abroad under age of 45 REQUIREMENTS: (1) Submit the title and abstract of your paper if you intend to participate in the symposium in receipt of the first announcement. In case the work is being conducted currently, a preliminary title is required.(Topic and abstract can be either in English or Chinese of any length) (2) Fill in the registration form if intends to participate in the Symposium and send it back together with a copy of curriculum vitae (3) IMPORTANT DATE: Deadline for the submission of the registration form is May 25. (4) Chinese scholars abroad please kindly forward this announcement to the person showing interest. 4. FEES: (1) Registration fee: Abroad: $200.00 US Home: RMB 400.00 (2) Food and lodge will be provided free during the Symposium to the participants who pay the registration fee (3) Financial support will be given to some of the scholars abroad based on the scientific background. Food and lodge are free for all participants during the Symposium 5. SCIENTIFIC ADVISOR: Members of the Chinese academy of sciences: Profs. Chen-Lu Tsou, Dong-Cai Liang and Pei-song Tang 6. ACADEMIC COMMITTEE(in alphabetic order) Jian-wen Chen, Xue-hai Han, Kun-sheng Hu, Fen Huang, You-guo Huang, Ting-yun Kuang, Ke-chun Lin, Qi-shui Lin, Zhi-huan Lin, Shu-sen Liu, Hua-zhen Pan, Sen-fang Sui, Wei Xu, Fu-yu Yang, Nan-ming Zhao 7. ORGANIZING COMMITTEE Honory Chairman: F.Y. Yang(Fu-yu Yang)(Member of the Chinese Academy of Sciences) Chairman: Prof. Youguo Huang Secretary in Chief: Dr. Xue-hai Han Organizing Committee(in alphabetic order): Dong Chen, Xue-hai Han, You-guo Huang, Ke-ding Lu, Xiu-zhen Lu, Hai-guan Ma, Yan Tian, Gu-yan Wang, Gui-hai Wang, Zhi-xin Wang, Xiao-ke Xia, Gen-jun Xu, Zheng-zong Yang, Jun-mei Zhou Secretariat:(in alphabetic order) Hui-juan Chen, Guang-Hu, Yu-lan Tian, Yi-bing Zheng, Wen-min Zhong 8. ADDRESS FOR COMMUNICATIONS: Hui-juan Chen Institute of Biophysics, Academia Sinica 15 Datun Rd, Chao yang District Beijing 100101, P.R. China Tel: 0086-010-62020077(extention 444) Fax: 0086-010-62022026 E-mail: yangfy@sun5.ibp.ac.cn huang@sun5.ibp.ac.cn ---------------------------------------------------------------------------- -- REGISTRATION FORM NAME(Chinese) (English) Sex Age Professional title Degree Institution/Co. Preliminary title Tel. Fax E-mail Apply for financial support: yes no Signature Date: ---------------------------------------------------------------------------- -- From owner-7tms_r@net.bio.net Sat Mar 22 22:00:00 1997 Path: biosci!agate!howland.erols.net!newsfeed.internetmci.com!feeder.chicago.cic.net!cougar.olivet.edu!usenet From: IRST Newsgroups: bionet.molbio.proteins.7tms_r Subject: IRST Needs your help. Date: Tue, 18 Mar 1997 22:31:36 -0600 Organization: Olivet Nazarene University Lines: 108 Message-ID: <332F6C27.6FD4@eggbeater.olivet.edu> NNTP-Posting-Host: r330a.chapman.olivet.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 4.0b2 (Win95; I) X-Priority: 3 (Normal) Dear Internet User, By now you've probably heard about the current issues pertaining to free speech and the regulation of Internet related materials. We, The Internet Regulatory Survey Team (IRST), are conducting a survey/study pertaining to these issues and are interested in your opinions. We are sending along a small 16 question survey that should take no more than five minutes of your time to complete. We ask for your participation in this study and provide us with your information. Your response will be completely anonymous. If you'd prefer to visit our Web site and fill out the form there the address is: http://eggbeater.olivet.edu/~survey/ Thank You in advance for your time! ----------------------------------------------- Internet Regulatory Survey 1. What service do you use to access the Internet? AOL Compuserve MSN Netcom Local Internet Service Provider (ISP) College or University LAN Other 2. How many times do you use Email during the week? 0-5 6-10 11-15 16-20 20+ 3. How many hours during the week do you spend on the Internet? 0-5 6-10 11-15 16-20 20+ 4. How long have you been using the Internet? 1-6 months 7-12 months 1-2 years 2-3 years 3+ years 5. How would you rate yourself as an Internet user? Beginner Intermediate Advanced Expert For questions 6-11 please indicate the level of agreement or disagreement for each. SA=Strongly Agree A=Agree N=Neutral D=Disagree SD=Strongly Disagree 6. The government should be allowed to regulate the Internet in general. SA A N D SD 7. How do you feel about laws governing material displayed on web sites? SA A N D SD 8. How do you feel about Internet service providers censoring email? SA A N D SD 9. A web site should be able to contain any information it wants no matter how vulgar, obscene, or derogatory it may be. SA A N D SD 10. Web sites should display a warning if they contain any material that may be questionable or offensive to others. SA A N D SD 11. All web sites should register their site with an Internet Censor Rating (ICR) company and display a censor rating, much like that used in television. SA A N D SD 12. Gender: Male / Female 13. Age: 14. Country: __________________ 15. Religious affiliation: Catholic Protestant Jewish Islamic Hindu Buddhist Other None 16. Political party: (U.S. Residents) Democrat Republican Independent Libertarian Other None (Non-US Residents) List political party name: _______________ ------------------------------------------------- We thank you for participation and encourage you to send us your comments and suggestions. Visit our Web site for more info Internet Regulatory Survey Team (IRST) WEB: http://eggbeater.olivet.edu/~survey/ EMAIL: survey@eggbeater.olivet.edu From owner-7tms_r@net.bio.net Mon Mar 24 22:00:00 1997 Path: biosci!MC.DUKE.EDU!daaka001 From: daaka001@MC.DUKE.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: separating closely related proteins Date: 25 Mar 1997 10:51:40 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Distribution: world Message-ID: <01IGX73EX830006F4L@mc.duke.edu> NNTP-Posting-Host: net.bio.net I've been trying to blot the two froms of Raf (74 and 74.5 kDa) with no appreciable success. Does anyone have any suggestion on how to proceed? Are there "special" SDS-PAGE recipes for this purpose? All inputs are greatfully appreciated. Yehia Daaka, daaka001@mc.duke.edu From owner-7tms_r@net.bio.net Mon Mar 24 22:00:00 1997 Path: biosci!DELISI.BU.EDU!kapi From: kapi@DELISI.BU.EDU (Rakefet Rosenfeld) Newsgroups: bionet.molbio.proteins.7tms_r Subject: subscribe 7TMS_R Date: 25 Mar 1997 23:34:52 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <3338CDAA.167E@delisi.bu.edu> NNTP-Posting-Host: net.bio.net subscribe 7TMS_R From owner-7tms_r@net.bio.net Mon Mar 24 22:00:00 1997 Path: biosci!DELISI.BU.EDU!kapi From: kapi@DELISI.BU.EDU (Rakefet Rosenfeld) Newsgroups: bionet.molbio.proteins.7tms_r Subject: internalization of GPCR Date: 25 Mar 1997 23:35:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 5 Sender: daemon@net.bio.net Distribution: world Message-ID: <3338CD68.41C6@delisi.bu.edu> NNTP-Posting-Host: net.bio.net I am interested in the process of receptor internalization and would like to know if this is a known/common phenomenon in GPCRs. Thank you, R. Rosenfeld From owner-7tms_r@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!ALICE.BAKER.EDU.AU!walter.thomas From: walter.thomas@ALICE.BAKER.EDU.AU (WalterThomas) Newsgroups: bionet.molbio.proteins.7tms_r Subject: non-internalizing GPCR Date: 26 Mar 1997 13:38:35 -0800 Organization: Baker Institute, Melb., Australia Lines: 35 Sender: daemon@net.bio.net Distribution: world Message-ID: <68AB1233661@alice.baker.edu.au> NNTP-Posting-Host: net.bio.net Bryan Roth asked: >Is anyone aware of an instance in which internalization following agonist >exposure does not occur? Interestingly, the peptide hormone angiotensin II promotes the endocytosis of the type 1 (AT1) angiotensin receptor but not the type 2 angiotensin (AT2) receptor. We have generated chimeric AT2/AT1 receptors where the carboxyl-terminus and 3rd intracellular loop of the internalizing AT1 receptor (these regions have been shown to be important for endocytosis) were substituted into the AT2 receptor. These modified AT2 receptors also failed to internalize following angiotensin II stimulation suggesting that additional determinants are required. People have also noted the robust internalization of AT1 receptors following binding of a peptide antagonist of angiotensin II (namely Sar1-Ile8-angiotensin II). Does anyone know of other examples were antagonists have been shown to cause internalization of GPCRs? All the best, Wally Walter G. Thomas Walter.Thomas@baker.edu.au Baker Medical Research Institute Commercial Rd. Prahran, Melbourne Victoria 3181 Australia International Phone 61-3-95224333 International Fax 61-3-95211362 From owner-7tms_r@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!FARMR1.MED.UTH.TMC.EDU!aschonb From: aschonb@FARMR1.MED.UTH.TMC.EDU ("A. Schonbrunn") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: internalization of GPCR Date: 26 Mar 1997 08:39:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 45 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <199703261458.OAA26474@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: net.bio.net >Is anyone aware of an instance in which internalization following agonist >exposure does not occur? At least one of the somatostatin receptor subtypes does not internalize ligand rapidly. see J. Cell Biology 102: 878, 1986 JBC 261: 3571, 1986 Agi Schonbrunn >>I am interested in the process of receptor internalization and would >>like to know if this is a known/common phenomenon in GPCRs. >> >>Thank you, >>R. Rosenfeld > > >We recently demontrated this for 5-HT2A receptors (Mol Pharmacol, 1996); its >also been shown for beta2-adrenergic receptors, thrombin receptors, >substance P receptors, muscarinic receptors, angiotensin and opiate >receptors. So I guess its common enough. > >Is anyone aware of an instance in which internalization following agonist >exposure does not occur? > >============================================================= > >Bryan L.Roth MD, PhD >Associate Professor >Departments of Psychiatry, Biochemistry and Neurosciences >Room W438 >Case Western Reserve University Medical School >10900 Euclid Avenue >Cleveland, OH 44106-4935 > >216-368-4544 (Fax) >216-368-2730 (Office) >roth@biocserver.cwru.edu > >web page: http://www.cwru.edu/CWRU/Dept/Med/biochemistry/faculty/roth.html From owner-7tms_r@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!BIOCSERVER.BIOC.CWRU.EDU!roth From: roth@BIOCSERVER.BIOC.CWRU.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: internalization of GPCR Date: 26 Mar 1997 06:57:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 31 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703261458.OAA26474@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: net.bio.net >I am interested in the process of receptor internalization and would >like to know if this is a known/common phenomenon in GPCRs. > >Thank you, >R. Rosenfeld We recently demontrated this for 5-HT2A receptors (Mol Pharmacol, 1996); its also been shown for beta2-adrenergic receptors, thrombin receptors, substance P receptors, muscarinic receptors, angiotensin and opiate receptors. So I guess its common enough. Is anyone aware of an instance in which internalization following agonist exposure does not occur? ============================================================= Bryan L.Roth MD, PhD Associate Professor Departments of Psychiatry, Biochemistry and Neurosciences Room W438 Case Western Reserve University Medical School 10900 Euclid Avenue Cleveland, OH 44106-4935 216-368-4544 (Fax) 216-368-2730 (Office) roth@biocserver.cwru.edu web page: http://www.cwru.edu/CWRU/Dept/Med/biochemistry/faculty/roth.html From owner-7tms_r@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!MAIL.MED.UPENN.EDU!Christoo From: Christoo@MAIL.MED.UPENN.EDU ("Chris O'Brien") Newsgroups: bionet.molbio.proteins.7tms_r Subject: SUBSCRIBE 7TMS Date: 26 Mar 1997 18:08:45 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <333A009E.56B8@mail.med.UPENN.edu> NNTP-Posting-Host: net.bio.net SUBSCRIBE 7TMS From owner-7tms_r@net.bio.net Tue Mar 25 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!uwm.edu!newsfeeds.sol.net!hammer.uoregon.edu!news-peer.gsl.net!news.gsl.net!news.maxwell.syr.edu!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!portc01.blue.aol.com!newsstand.cit.cornell.edu!cu-dialup-0721.cit.cornell.edu!user From: fjk1@cornell.edu (Jack Kou) Newsgroups: bionet.molbio.proteins.7tms_r Subject: help! production procedures for thyroprotein / iodine casein Date: Thu, 27 Mar 1997 01:38:26 -0500 Organization: Cornell Lines: 27 Sender: fjk1@cornell.edu (Verified) Message-ID: NNTP-Posting-Host: cu-dialup-0721.cit.cornell.edu I would like to obtain the production procedure for making thyroprotein / iodine casein in a commercial setting. I have the molecular structure (hard to post) and the following information: Molecular Weight = 776.93 C: 23.19% H: 1.43% O: 8.24% N: 1.80% I: 65.34% insoluable in water; soluable in N-Butanol & OH base solutions. organic iodine: 9 ~ 10% inorganic iodine: - melting point: 231 - 233° C :­4.4 (3% in 0.13N NaOH in 70% EtOH) Please reply even if you know only of some source where I might get more information. sincere appreciation. jack kou fjk1@cornell.edu From owner-7tms_r@net.bio.net Wed Mar 26 22:00:00 1997 Path: biosci!BIOCSERVER.BIOC.CWRU.EDU!roth From: roth@BIOCSERVER.BIOC.CWRU.EDU Newsgroups: bionet.molbio.proteins.7tms_r Subject: non-internalizing 7TM receptors Date: 27 Mar 1997 07:58:00 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 21 Sender: daemon@net.bio.net Distribution: world Message-ID: <199703271558.PAA00865@biocserver.BIOC.CWRU.Edu> NNTP-Posting-Host: net.bio.net Many folks have sent me examples of non-internalizing 7TM receptors. I'll summarize the info next week for all members of this list. Thanks! ============================================================= Bryan L.Roth MD, PhD Associate Professor Departments of Psychiatry, Biochemistry and Neurosciences Room W438 Case Western Reserve University Medical School 10900 Euclid Avenue Cleveland, OH 44106-4935 216-368-4544 (Fax) 216-368-2730 (Office) roth@biocserver.cwru.edu web page: http://www.cwru.edu/CWRU/Dept/Med/biochemistry/faculty/roth.html From owner-7tms_r@net.bio.net Wed Mar 26 22:00:00 1997 Path: biosci!ihnp4.ucsd.edu!munnari.OZ.AU!news.ecn.uoknor.edu!feed1.news.erols.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.nacamar.de!wuff.mayn.de!wuff.franken.de!winx03!wpxx02!not-for-mail From: krasel@wpxx02.toxi.uni-wuerzburg.de (Cornelius Krasel) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: internalization of GPCR Date: Thu, 27 Mar 1997 11:47:53 +0100 Organization: University of Wuerzburg, Germany Lines: 46 Distribution: world Message-ID: References: <3338CD68.41C6@delisi.bu.edu> NNTP-Posting-Host: wpxx02.toxi.uni-wuerzburg.de X-Newsreader: TIN [UNIX 1.3 950824BETA PL0] Rakefet Rosenfeld (kapi@DELISI.BU.EDU) wrote: > I am interested in the process of receptor internalization and would > like to know if this is a known/common phenomenon in GPCRs. It is known, yes. It is also (fairly) common. It proceeds at least for some receptor types in some cells through phosphorylation of the receptor, binding to beta-arrestin, interaction of the beta-arrestin with clathrin and internalization via clathrin-coated vesicles (CCVs). @article{ferguson:96, author = {Stephen S. G. Ferguson and William E. {Downey III} and Anne-Marie Colapietro and Larry S. Barak and Luc M{\'e}nard and Marc G. Caron}, title = {Role of $\beta$-Arrestin in Mediating Agonist-Promoted {G} Protein-Coupled Receptor Internalization.}, journal = {Science}, volume = 271, pages = {363--366}, year = 1996 } @article{goodman:96, author = {Oscar B. {Goodman, Jr.} and Jason G. Krupnick and Francesca Santini and Vsevolod V. Gurevich and Raymond B. Penn and Alison W. Gagnon and James H. Keen and Jeffrey L. Benovic}, title = {$\beta$-{A}rrestin acts as a clathrin adaptor in endocytosis of the $\beta_2$-adrenergic receptor.}, journal = {Nature}, volume = 383, pages = {447--450}, year = 1996 } In other cell lines or with other receptors, clathrin does not seem to be involved (assessed by the effects of a dominant-negative dynamin mutant which blocks internalization via CCVs). There are also GPCRs which are resistant to internalization. --Cornelius. -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP3 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Wed Mar 26 22:00:00 1997 Path: biosci!VIOLET.INCM.U-NANCY.FR!poda From: poda@VIOLET.INCM.U-NANCY.FR (Gennady PODA) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 7tms_r: CXCR3 sequence Date: 27 Mar 1997 06:08:12 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, Does anybody know any synonyms for beta chemokine CXCR-3 receptor? Or where can I retrieve its sequence (any specie). I failed to find its sequence using the name "CXCR-3" (or chemokine receptor) in several sequence databases (SwissProt, EMBL, etc). Thank you very much beforehand for your help. With best wishes, Gennady ************************************************************ Dr. Gennady PODA Laboratoire de Chimie theorique (UA 510 du C.N.R.S.) Universite Henri Poincare, Nancy-I Faculte des Sciences - Domaine scientifique Victor Grignard B.P. 239 - 54500 Vandoeuvre-les-Nancy Cedex France Fax : + 33 (0)3.83.91.25.30 E-mail : poda@incm.u-nancy.fr ************************************************************ From owner-7tms_r@net.bio.net Wed Mar 26 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!arclight.uoregon.edu!news.sprintlink.net!news-peer.sprintlink.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsxfer3.itd.umich.edu!portc01.blue.aol.com!newsstand.cit.cornell.edu!inkblot.med.cornell.edu!news-admin@mail.med.cornell.edu From: Lonny Levin Newsgroups: bionet.molbio.proteins.7tms_r Subject: Cannabinoid R KO? Date: Thu, 27 Mar 1997 10:54:47 -0500 Organization: Cornell University Medical College Lines: 22 Message-ID: <333A97E6.704B@mail.med.cornell.edu> NNTP-Posting-Host: mac102949.med.cornell.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0Gold (Macintosh; I; PPC) CC: yqchen@Mail.med.cornell.edu Hello, Does anyone know of a knockout of the Cannabinoid Receptor? Has it been done or is it being done? Please respond directly to me or to yqchen@Mail.med.cornell.edu THANK YOU -- **************************************************** Lonny R. Levin Department of Pharmacology Cornell University Medical College 1300 York Ave. Rm. E-505 New York, NY 10021 Office: 212-746-6752 Lab: 212-746-6750 FAX: 212-746-8835 LLEVIN@mail.med.cornell.edu www-users.med.cornell.edu/~llevin/default.html **************************************************** From owner-7tms_r@net.bio.net Thu Mar 27 22:00:00 1997 Path: biosci!bcm.tmc.edu!cs.utexas.edu!howland.erols.net!worldnet.att.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.nacamar.de!fu-berlin.de!gmanlab2.ukbf.fu-berlin.DE!not-for-mail From: Michael Schaefer Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: 7tms_r: CXCR3 sequence Date: Fri, 28 Mar 1997 12:52:57 +0100 Organization: Dept. of Pharmacology, Free University Berlin, Germany Lines: 10 Message-ID: <333BB119.434C@zedat.fu-berlin.de> References: Reply-To: Thielallee, 69-73, D-14195, Berlin NNTP-Posting-Host: gmanlab2.ukbf.fu-berlin.de (160.45.194.75) Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Access: 16 17 19 X-Mailer: Mozilla 3.0 (Win95; I) To: Gennady PODA Gennady PODA wrote: > > Dear Netters, > > Does anybody know any synonyms for beta chemokine CXCR-3 receptor? Or > where can I retrieve its sequence (any specie). I failed to find its > sequence using the name "CXCR-3" (or chemokine receptor) in several > sequence databases (SwissProt, EMBL, etc). Just try the accession No. U29677. I hope it matches. From owner-7tms_r@net.bio.net Sat Mar 29 22:00:00 1997 Path: biosci!rutgers.rutgers.edu!gatech!csulb.edu!newshub.csu.net!news.ironhorse.com!mr.net!news-peer.gsl.net!news.gsl.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.nacamar.de!fu-berlin.de!gmanlab2.ukbf.fu-berlin.DE!not-for-mail From: Michael Schaefer Newsgroups: bionet.molbio.proteins.7tms_r Subject: Recontitution of 7TMD-R into living cells possible? Date: Sun, 30 Mar 1997 18:19:16 +0200 Organization: Dept. of Pharmacology, Free University Berlin, Germany Lines: 11 Message-ID: <333E9284.32C4@fub46.zedat.fu-berlin.de> Reply-To: Thielallee, 69-73, D-14195, Berlin NNTP-Posting-Host: gmanlab2.ukbf.fu-berlin.de (160.45.194.75) Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Access: 16 17 19 X-Mailer: Mozilla 3.0 (Win95; I) We are working about signalling pathways in motile cells that are not accessible for electrophysiological and other single cell-based tecniques. Therfore my questions: Is it possible to integrate 7TMD-receptor-containing membranes into living cells by microinjecton or other tecniques? Has anybody practical experience with such a system? Is the Xenopus oocyte suitable to inject membrane fragments or solubilized membrane proteins that reintegrate into the membranes in a functional manner? Many thanks in advance, Michael Schaefer. From owner-7tms_r@net.bio.net Sun Mar 30 23:00:00 1997 Path: biosci!agate!howland.erols.net!EU.net!news.eunet.fi!online.tietokone.fi!poweiurowipeurowe From: poweiurowipeurowe@qoiuarweopurowpe.com Newsgroups: bionet.molbio.proteins.7tms_r Subject: Salma Hayek Nude Date: Sun, 30 Mar 1997 17:03:25 +0300 Organization: Tietokone Online Lines: 22 Sender: poweiurowipeurowe@qoiuarweopurowpe.com Message-ID: References: <5hlrrd$heh@bolivia.earthlink.net> NNTP-Posting-Host: posti.tietokone.fi Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Content-ID: X-Gateway: NASTA Gate 1.17 for FirstClass(R) From: poweiurowipeurowe@qoiuarweopurowpe.com Subject: Salma Hayek Nude Newsgroups: bionet.molbio.proteins.7tms_r Date: 30 Mar 1997 14:03:25 GMT Check out this site, it has tons of Nude Celebrity pics. http://www.sexy-stars.com or if you like young slutty teens go here: http://www.sexy-girls.com Everybody needs some sex in there life. Message-ID: <5hlrrd$heh@bolivia.earthlink.net> NNTP-Posting-Host: 207.217.19.89 Organization: Earthlink Network, Inc. From owner-7tms_r@net.bio.net Sun Mar 30 23:00:00 1997 Path: biosci!agate!howland.erols.net!ais.net!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!EU.net!news.eunet.fi!online.tietokone.fi!schae From: schae@fub46.zedat.fu-berlin.de Newsgroups: bionet.molbio.proteins.7tms_r Subject: Recontitution of 7TMD-R into living cells possible? Date: Sun, 30 Mar 1997 19:19:16 +0300 Organization: Tietokone Online Lines: 24 Sender: schae@fub46.zedat.fu-berlin.de Message-ID: References: <333E9284.32C4@fub46.zedat.fu-berlin.de> NNTP-Posting-Host: posti.tietokone.fi Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 Content-Transfer-Encoding: 8bit Content-ID: X-Gateway: NASTA Gate 1.17 for FirstClass(R) From: Michael Schaefer Subject: Recontitution of 7TMD-R into living cells possible? Newsgroups: bionet.molbio.proteins.7tms_r Date: Sun, 30 Mar 1997 18:19:16 +0200 We are working about signalling pathways in motile cells that are not accessible for electrophysiological and other single cell-based tecniques. Therfore my questions: Is it possible to integrate 7TMD-receptor-containing membranes into living cells by microinjecton or other tecniques? Has anybody practical experience with such a system? Is the Xenopus oocyte suitable to inject membrane fragments or solubilized membrane proteins that reintegrate into the membranes in a functional manner? Many thanks in advance, Michael Schaefer. 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From owner-7tms_r@net.bio.net Mon Mar 31 23:00:00 1997 Path: biosci!MEDICINE.WUSTL.EDU!ongurd From: ongurd@MEDICINE.WUSTL.EDU (Dost Ongur) Newsgroups: bionet.molbio.proteins.7tms_r Subject: SUBSCRIBE Date: 1 Apr 1997 08:07:59 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704011608.KAA14572@medicine.wustl.edu> NNTP-Posting-Host: net.bio.net SUBSCRIBE From owner-7tms_r@net.bio.net Mon Mar 31 23:00:00 1997 Path: biosci!MEDICINE.WUSTL.EDU!ongurd From: ongurd@MEDICINE.WUSTL.EDU (Dost Ongur) Newsgroups: bionet.molbio.proteins.7tms_r Subject: SUBSCRIBE 7TMS Date: 1 Apr 1997 09:26:17 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: <199704011726.LAA00056@medicine.wustl.edu> NNTP-Posting-Host: net.bio.net SUBSCRIBE 7TMS