From owner-7tms_r@net.bio.net Mon Jun 02 23:00:00 1997 Path: biosci!agate!newsfeed.kornet.nm.kr!news.maxwell.syr.edu!news-feed.inet.tele.dk!uninett.no!news.uit.no!not-for-mail From: edvard@fagmed.uit.no () Newsgroups: bionet.molbio.proteins.7tms_r Subject: GRAP databases unavailable Date: 3 Jun 1997 08:56:20 GMT Organization: Univ. of Tromso, IMB Lines: 21 Message-ID: <5n0m7k$mdk$1@news.uit.no> Reply-To: edvard@fagmed.uit.no NNTP-Posting-Host: fa13.imb.fm.uit.no The GRAP databases will be unavailable from NOW and until further notice due to various upgrades of data and software. Øyvind. -- -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Mon Jun 02 23:00:00 1997 Path: biosci!daresbury!uninett.no!news.uit.no!not-for-mail From: edvard@fagmed.uit.no (Øyvind Edvardsen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: tinyGRAP release 5.0 Date: 3 Jun 1997 14:22:08 GMT Organization: Univ. of Tromso, IMB Lines: 42 Message-ID: <5n19ag$4u$1@news.uit.no> Reply-To: edvard@fagmed.uit.no NNTP-Posting-Host: fa13.imb.fm.uit.no Release 5.0 of tinyGRAP is now ready. The major focus of this release is the inclusion of Deletion mutants. We have extracted deletion mutants from the papers already available in the database and entered the mutant information by looking at a deletion as a substitution of an amino acid into an 'amino acid' called DEL. Thus, much of the software etc. already available could be used unaltered. However, many of the deletion mutants span much longer stretches than any of the multiple substitution mutants, and therefore the deletion mutants have a different list-format than usual: the amino acid sequence deleted is shown. The side effect of all this is a somewhat worse performance regarding the search speed and production of an answer document (list of matching mutants). We'll continue to work to reduce the response time. * We have added ~270 deletion mutants * We have added ~60 substitution mutants * We have added only a few new literature references * We have corrected a number of literature references * The links into Medline performs much better (~1.5% failure) than before (In fact, for some time none of them were working. Thanks to Fabien Champagne for the notification that led us to do the necessary changes and optimization.) We plan the next release of tinyGRAP in september, and will then focus on recent papers dealing with mutant GPCRs. Øyvind. -- -o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o-o- ___ _ | /| _| |_ _| __ _ _| _ _ |/_| \/ \/ | |\| |_| |_ |_| \/ |_|_ | |_| _\ |_' |\| / _____________________________________________________________________________ School of Medicine | Dept. of Pharmacology, IMB | TelePhone: +47 77 64 53 42 University of Tromsoe | TeleFax: +47 77 64 53 10 MH, Breivika | Email: edvard@fagmed.uit.no N-9037 TROMSOE, NORWAY | URL: http://atf1.fagmed.uit.no/mgl.html ------------------------------------------------------------------------------ From owner-7tms_r@net.bio.net Wed Jun 04 23:00:00 1997 Path: biosci!YALE.EDU!henrik.dohlman From: henrik.dohlman@YALE.EDU (Henrik Dohlman) Newsgroups: bionet.molbio.proteins.7tms_r Subject: mislocalized receptors? Date: 5 Jun 1997 11:15:53 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 17 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi folks, One of the causes of Retinitis Pigmentosa is the accumulation of mutant rhodopsin in the ER, resulting in retinal degeneration. Is anyone aware of mutants in other receptors (either naturally occuring or artificially constructed) that cause them to be mislocalized within the cell? Many thanks, Henrik Henrik G. Dohlman, PhD Yale University School of Medicine henrik.dohlman@yale.edu From owner-7tms_r@net.bio.net Sun Jun 08 23:00:00 1997 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!news-was.dfn.de!news-fra1.dfn.de!news-ber1.dfn.de!fu-berlin.de!schupha8.pharmazie.fu-berlin.DE!not-for-mail From: Holger Stark Newsgroups: bionet.molbio.proteins.7tms_r Subject: phylogenetic tree Date: Mon, 09 Jun 1997 16:44:11 +0200 Organization: Freie Universitaet Berlin, Inst. f. Pharmazie I Lines: 30 Message-ID: <339C16BB.8FC9363E@schunet1.pharmazie.fu-berlin.de> NNTP-Posting-Host: schupha8.pharmazie.fu-berlin.de (160.45.56.107) Mime-Version: 1.0 Content-Type: text/plain; charset=iso-8859-1 X-Access: 16 17 19 X-Mailer: Mozilla 4.0b5 [en] (Win95; I) X-Priority: 3 (Normal) For a student lecture I am looking for a good picture of a phylogenetic tree of G protein coupled receptors (7 TM receptors, may be special interest amine receptors). I would like to get a dendritic tree, but a parallel one would also be great. May be anyone knows where I can get it. Please, send me a short message.... Thank you Holger PS. I already found a reprint in TiPS, but scanning of this picture resulted in loss of information. -- ____________________________________________________ Dr. Holger STARK Freie Universitaet Berlin Institut fuer Pharmazie I Koenigin-Luise-Str. 2+4 D-14195 Berlin, GERMANY Tel.: +49 - 30 - 838 3295/3928 Fax: +49 - 30 - 838 3854 E-mail: stark@schunet1.pharmazie.fu-berlin.de Home page: http://www.pharmazie.fu-berlin.de _____________________________________________________ From owner-7tms_r@net.bio.net Tue Jun 10 23:00:00 1997 Path: biosci!BIO.VU.NL!ahdeboer From: ahdeboer@BIO.VU.NL ("A.H. de Boer") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoc (14-3-3 proteins) open in Amsterdam Date: 11 Jun 1997 15:44:13 -0700 Organization: Vrije Universiteit Amsterdam Lines: 44 Sender: daemon@net.bio.net Distribution: world Message-ID: <339FA9AF.7B0A@bio.vu.nl> NNTP-Posting-Host: net.bio.net A postdoctoral position is available to investigate the role of 14-3-3 proteins in the regulation of the plant plasma membrane H+-ATPase and nitrate reductase, an enzyme which is located in the cytosol. 14-3-3 proteins play a key role in coordinating multiple signalling pathways (see Aitken 1996, Trends Cell Biol. 6, 341-347) by serving as docking proteins for receptors, kinases and phosphatases. In plants some 14-3-3 isoforms make up the receptor for the phytotoxin fusicoccin (see de Boer 1997, Trends Plant Sci. 2, 60-66). Plants have a 14-3-3 gene family with 6 to 10 members and it has been speculated that one isoform has more than one function. Using a combination of biochemical, genetic and molecular approaches we want to address the question which isoforms bind to (and control the activity of) the ATPase and nitrate reductase respectively. Since activation of root nitrate reductase also activates the ATPase, 14-3-3 dimers may mediate the ‘cross-talk' between these two enzymes. The dynamic behaviour of 14-3-3 isoforms (viz. translocation within the cell and docking to the respective targets proteins) will be studied by means of Green Fuorescent Protein (GFP) constructs. This project, is part of an EU Framework IV Biotech-programme entitled ‘Central Role in Adaptation of Fourteen Three Three proteins', and is carried out by six international partners. In the lab you will be part of a group of 3 postdocs working on 14-3-3 proteins in barley and Petunia. Candidates should have demonstrated experience and publications in biochemistry/protein chemistry and molecular biology. Experience with microscopic techniques is considered favourably. The position will be for a period of 3 years in the Department of Genetics, Section Plant Physiology of The Vrije Universiteit Amsterdam, The Netherlands. Applicants should send (before July 10) a curriculum vitae, a brief statement of research interest and names and phone numbers and e-mail address of 3 references to: Bert de Boer Faculty of Biology, Vrije Universiteit Amsterdam Dep. Of Genetics, Section Plant Physiology De Boelelaan 1087 1081 HV Amsterdam, the Netherlands. Phone: 31-20- 444 7162 Fax: 31-20-444 7229 E-mail: ahdeboer@bio.vu.nl PS: I am out of the country until July 5th, but after that I will be happy to give more information about the position. From owner-7tms_r@net.bio.net Wed Jun 11 23:00:00 1997 Path: biosci!internet!biosci!not-for-mail From: biohelp (BIOSCI Administrator) Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Jun 1997 02:00:07 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Distribution: world Message-ID: <199706120900.CAA10849@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI ------------------------------------------------------------------ node at computer net.bio.net: ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. C) In the body of your message put one or more of the following commands with an "end" command on the last line, e.g., subscribe methods unsubscribe methods end Do NOT put your e-mail address or other text on these lines. The server only allows you to cancel your subscription if the address on your mail header matches the address on our mailing list. Please ask for help at biosci-help@net.bio.net if your address has changed, e.g., if you know you are on the list but the server tells you that you are not a member. Users in Europe, Africa, and Central Asia who use the BIOSCI node at -------------------------------------------------------------------- computer daresbury.ac.uk (also known as dl.ac.uk): ------------------------------------------------- To subscribe and unsubscribe to/from the BIOSCI lists, you need to specify the full USENET newsgroup name with "bionet-news." prepended. The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. From owner-7tms_r@net.bio.net Thu Jun 12 23:00:00 1997 Path: biosci!rutgers!gatech!news-out.communique.net!communique!news-spur1.maxwell.syr.edu!news.maxwell.syr.edu!news-peer.gsl.net!uwm.edu!newsspool.doit.wisc.edu!news.doit.wisc.edu!news From: cmneira@facstaff.wisc.edu (Mauricio Neira) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Olfactory Receptors Date: Fri, 13 Jun 1997 02:46:34 GMT Organization: University of Wisconsin Lines: 16 Message-ID: <33a0b0fc.8658621@news.doit.wisc.edu> Reply-To: cmneira@facstaff.wisc.edu NNTP-Posting-Host: f183-047.net.wisc.edu X-Newsreader: Forte Agent .99d/32.182 Hi! I would like to use a tissue for RNA extraction or a cDNA library from any organism containing olfactory receptors...however I don't know how to get this...is there a company selling such things? or anyone who could share such samples? I am in a Mol. Biol Laboratory and currently we don't work with animals...so that preparing the tissue by myself will not be very simple...however I will try if there is no other possibility...could be that is not so difficult... I appreciate any suggestions... Thanks! Mauricio Neira, Ph.D. University of Wisconsin Department of Medicine From owner-7tms_r@net.bio.net Thu Jun 12 23:00:00 1997 Path: biosci!monsanto.com!JMSTAU From: JMSTAU@monsanto.com Newsgroups: bionet.molbio.proteins.7tms_r Subject: UNSUBSCRIBE, PLEASE Date: 13 Jun 1997 12:59:14 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 1 Sender: daemon@net.bio.net Distribution: world Message-ID: <0030400004194170000002*@MHS> NNTP-Posting-Host: net.bio.net UNsubscribe PLEASE!! From owner-7tms_r@net.bio.net Thu Jun 12 23:00:00 1997 Path: biosci!agate!spool.mu.edu!uwm.edu!news.he.net!news2.fibr.net!news1.fibr.net!news.fibr.net!news From: Newsgroups: bionet.molbio.proteins.7tms_r Subject: http://www.planetfun.com/videosex.htm Date: 14 Jun 1997 05:49:45 GMT Lines: 3 Message-ID: <5ntbdp$eb3@nimitz.fibr.net> NNTP-Posting-Host: port1-6.lconn.com http://www.planetfun.com/videosex.htm Adults Only From owner-7tms_r@net.bio.net Thu Jun 12 23:00:00 1997 Path: biosci!MED.UNR.EDU!buxton From: buxton@MED.UNR.EDU ("Iain L. O. Buxton") Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: UNSUBSCRIBE Date: 13 Jun 1997 19:31:48 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 2 Sender: daemon@net.bio.net Distribution: world Message-ID: References: <0030400004194170000002*@MHS> NNTP-Posting-Host: net.bio.net unsubscribe From owner-7tms_r@net.bio.net Thu Jun 12 23:00:00 1997 Path: biosci!bms.com!watson_j From: watson_j@bms.com (John Watson) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: UNSUBSCRIBE, PLEASE Date: 13 Jun 1997 14:48:04 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net JMSTAU@monsanto.COM wrote: > UNsubscribe PLEASE!! Don't send your subscribe/unsubscribe requests to the newsgroup. rather, do the following (from the famous Biosci FAQ): If you ever want to remove yourself from this mailing list, send the following command in email to "biosci-server@net.bio.net": unsubscribe 7tms_r yourname@youraddress Cheers, AJW From owner-7tms_r@net.bio.net Sun Jun 15 23:00:00 1997 Path: biosci!MED.UNR.EDU!buxton From: buxton@MED.UNR.EDU ("Iain L. O. Buxton") Newsgroups: bionet.molbio.proteins.7tms_r Subject: unsubscribe Date: 16 Jun 1997 08:07:09 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 13 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net unsubscribe buxton@med.unr.edu Iain L. O. Buxton Professor of Pharmacology/318 Associate Dean for Research University of Nevada School of Medicine Howard Medical Sciences Building Rm:216 Reno, NV 89557 Phone: (702)784-1566 FAX: (702)784-1620 buxton@med.unr.edu From owner-7tms_r@net.bio.net Wed Jun 18 23:00:00 1997 Message-ID: <33A16EC5.4616@scicentral.com> Date: Fri, 13 Jun 1997 12:01:09 -0400 From: SciCentral Reply-To: scicentral@scicentral.com Organization: SciLink X-Mailer: Mozilla 3.0Gold (Win95; I) MIME-Version: 1.0 Newsgroups: bionet.molbio.oncogenes,bionet.molbio.plant,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r,bionet.molbio.proteins.fluorescent,bionet.molbio.rapd,bionet.molbio.recombination,bionet.molbio.yeast Subject: BEST MOLECULAR BIOLOGY DIRECTORIES Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-Host: 166.72.73.161 Lines: 35 Path: biosci!news.Stanford.EDU!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsm.ibm.net!ibm.net!news1.ibm.net!166.72.73.161 Xref: biosci bionet.molbio.proteins:11016 bionet.molbio.proteins.7tms_r:1290 bionet.molbio.proteins.fluorescent:1305 bionet.molbio.rapd:1952 bionet.molbio.recombination:491 bionet.molbio.yeast:7250 Dear Colleagues, We invite you to attend an opening in your honor at: http://www.scicentral.com This unique Web site has been created for scientists and engineers by scientists who know your needs. Just three clicks will get you everywhere. http://www.scicentral.com includes only the most valuable directories on the Web. They currently constitute a gateway to over 50,000 scientific sites pertaining to over 120 specialties in science and engineering ranging from health, biological, earth, physical, and engineering sciences, to government and institutional listings, and including the Commerce Business Daily and Medline--all free. We envision http://www.scicentral.com as the science and engineering hub of the World Wide Web, a place for professionals to gather and discuss the complex issues facing us all as we approach the 21st century. This is where you can make your opinions known. We plan editorials, chat rooms, news. We will continue to be responsive to your suggestions. Please visit http://www.scicentral.com often and bring your colleagues. Ellen S. Uffen, Ph.D. President, SciLink, Inc. Guy Orgambide, Ph.D. Chief Executive Officer SciLink, Inc. Robert L. Uffen, Ph.D Professor Emeritus Michigan State University From owner-7tms_r@net.bio.net Wed Jun 25 23:00:00 1997 Path: biosci!CHEM.VU.NL!vheerik From: vheerik@CHEM.VU.NL (Harm van Heerikhuizen) Newsgroups: bionet.molbio.proteins.7tms_r Subject: 2-hybrid between Galpha and GPCR? Date: 26 Jun 1997 01:23:24 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 27 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Dear Netters, In yeast there are two different Galpha proteins around: Gpa1p and Gpa2p. Gpa1p interacts with the mating type receptor. What the nature of the upstream partner of Gpa2p is, is not known; it might even not be a GPCR. I wondered whether it would be feasible to try and identify the protein that can activate Gpa2p by a 2-hybrid analysis. My question is: has anyone ever performed a 2-hybrid analysis to study the interaction between a G-protein and (a) GPCR(s). Would one expect the strength of such an interaction to be large enough to generate a positive signal in a 2-hybrid screen? Thanks! Harm van Heerikhuizen, PhD Dept. of Biochemistry and Molecular Biology Vrije Universiteit De Boelelaan 1083 1081 HV Amsterdam The Netherlands tel: +31 20 4447573 fax: +31 20 4447553 e-mail: vheerik@chem.vu.nl From owner-7tms_r@net.bio.net Wed Jun 25 23:00:00 1997 Path: biosci!ZJAU.EDU.CN!lidb From: lidb@ZJAU.EDU.CN (Li Debao) Newsgroups: bionet.molbio.proteins.7tms_r Subject: (no subject) Date: 26 Jun 1997 21:13:58 -0700 Organization: Zhou Xueping Lines: 118 Sender: daemon@net.bio.net Distribution: world Message-ID: <33B20D55.1B46@zjau.edu.cn> Reply-To: lidb@public.hz.zj.cn NNTP-Posting-Host: net.bio.net Dear Sir/Madam, Encloseed this E-mail id the 2nd announcement of the 3rd international symposium on plant pathology and biotechnology which will be held in Hangzhou, from Nov.2 - Nov.7 China. We hope you could attend the symposium, your contribution are appreciated to us and all of our precipitant very much. Many Thanks to your kindly reply Xu Jianping 3RD HANGZHOU INTERNATIONAL SYMPOSIUM ON PLANT PATHOLOGY AND BIOTECHNOLOGY HANGZHOU, P. R .CHINA (Second Announcement) November 2 - November 7, 1997 The 3rd Hangzhou International Symposium on Plant Pathology and Biotechnology will be held at the Biotechnology Institute, Zhejiang Agricultural University (BIZAU) from November 2 - November 7, 1997, sponsored by the Chinese Plant Pathology Society (CPPS) and National Natural Science Foundation of China (NNSFC). The Scientific Committee of Symposium Honor Chairman: Professor Liu, Yi., chairman of the Chinese Plant Pathology Society (CPPS) Chairman: Professor Li, Debao., vice chairman of the Chinese Plant Pathology Society and the director of BIZAU. Vice Chairman: Professor Zheng, Zhong., Dean of Plant Protection, Zhejiang Agricultural University Professor Xie, Lianghui., The member of Chinese Science Academic Sinica, Fujian Agricultural University Members: He, Liyuan., Zhang, Binxing., Wen, Fujiang., Jia, Shirong., Xu, Ping., Chen, Jianping., Zhou, Xueping., N. Talbot., Wang, Jisheng., Kang, Zhengsheng., Wang, Dafang., Chen, Yongxuan., Shun, Shuyuan., Tian, Fenxiu., Zhang, Zhongyi., Liu, Shongling., Chen Lihua. Secretariat ( Liaison Office) Zhou, Xueping, Biotechnology Institute, ZAU Xu, Jianping, Biotechnology Institute, ZAU Wu, Yuliang, Biotechnolgy Institute, ZAU Fax: +86-571-696 1525 Telephone: +86-571- 697 1182, 697 1184 E-mail: lidb@public.hz.zj.cn SCIENTIFIC PROGRAM Plenary Lectures: N. Talbot (Exeter University, UK): Molecular Genetics of Infection Related Development in Magnaporthe grisea Dong, J., (Novatis Seeds Company, USA): The Research Progress of Novel Genes of Disease Control of Vegetables, Fruits and Corn (Temporary) La, Yong-Joon., (College of Agriculture and Life Sciences, Seoul National University, Korea): Use of high-density latex agglutination test for detection of plant viruses Tien Po (Institute of Microbiology, CAS): Plant Virus Satellite RNA and its roles in Plant Virus Disease Control (Temporary) Tang Jiliang (Guanxi Agricultural University): The Pathogenesis of Bacterial Plant Pathogens Fang Rongxiang (Institute of Microbiology, CAS): Molecular Aspects of Rice Virus The plenary lectures may be changed in case. The scientific program may include some work groups as following: W01: Mechanisms of Disease Resistance, Plant-Pathogen Interaction and Molecular Aspects of Pathogenesis W02: Structure and Expression of Virus Genome, and Novel Approaches to Diagnosis and Detection of Pathogens and Diseases W03: Biotechnology of Plant Diseases, Transgenic Plants REGISTRATION: The symposium will be held at International Exchange Center of Zhejiang Agricultural University in the campus, individuals who can not register for symposium before October 20, 1997, can registrant at the opening session. The campus of ZAU is near to the famous sightseeing scenic spot Westlake, it is less than 20 minuets bus way to it. The 100 USD registration fee for foreigner and 500 RMB for Chinese is required. The accommodation includes food is approximately 40 $ per day. Graduate students pay half of the cost. REQUIREMENT OF PAPER Space is available for posters. Researchers with new results and data in the areas relevant to plant pathology and biotechnology are encouraged to submit abstracts for poster display to ensure the broadest possible coverage and exchange of recent advances. Please notice the deadline for the submission of the abstract is Aug. 15, 1997. One page abstract should be written in English, typed in single spaced using 10-12 characters per inch, side margined of 1 1/4 inches (3 cm) and printed in a size of A4 in duplicates. Please mail them with a copy of abstract as a Microsoft Word for Windows 95 or Windows 3.X or Macintosh file on a floppy diskette. Please be sure to indicate the authors and their mailing addresses and e-mail addresses. The poster board will be 30 X 40 inches (about 75 cm X 95 cm) for each one. The title should be printed with letters at least 1 inch (2.4 cm). The official invitation will be forwarded on request. SPECIAL SUPPORTS Limited finacial support will be avaliable, all or part of accommodation fee for limited authors might be exempted. From owner-7tms_r@net.bio.net Thu Jun 26 23:00:00 1997 Path: biosci!UNR.EDU!schooley From: schooley@UNR.EDU (David A. Schooley) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Moderate or unsubscribe?? Date: 27 Jun 1997 08:47:02 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 14 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I thought a decision had been made to moderate communications about a month ago. Since then, it seems the frequency of unsolicited mail has only increased, until it greatly exceeds the scientific communications. I have already seen a colleague from another department here unsubscribe. Is moderation really going to happen? David A. Schooley Dept. of Biochemistry/330 Univ. of Nevada Reno, NV 89557 schooley@med.unr.edu tel: (702) 784-4136; fax (702) 784-1419 From owner-7tms_r@net.bio.net Fri Jun 27 23:00:00 1997 Path: biosci!EMBL-HEIDELBERG.DE!Gert.Vriend From: Gert.Vriend@EMBL-HEIDELBERG.DE Newsgroups: bionet.molbio.proteins.7tms_r Subject: junk mail Date: 28 Jun 1997 08:57:59 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 12 Sender: daemon@net.bio.net Distribution: world Message-ID: <199706281558.RAA08037@nu> NNTP-Posting-Host: net.bio.net About a month ago, we decided to moderate this list. By now I am getting 10 times more mail about porno and how to get rich than serious mails. Does ANYBODY know what happened with the moderation plans (Frank?). If time is a problem for the moderator, please tell me. We can do this moderation at EMBL with three people which makes it less of a burden. Please do not respond to this mail by sending mails to the entire group, unless you t think the entire group should read it. Gert Vriend From owner-7tms_r@net.bio.net Mon Jun 30 23:00:00 1997 Path: biosci!pravda.ucr.edu!newsfeed2.aimnet.com!news.maxwell.syr.edu!newsfeed.internetmci.com!supernews.com!Supernews69!not-for-mail From: lkjlkj@lkjrlfwlu.com Newsgroups: bionet.molbio.proteins.7tms_r Subject: 1-800 SERVICE AT 7.9 CENTS PER MINUTE!!! Date: 1 Jul 1997 20:48:15 GMT Organization: All USENET -- http://www.Supernews.com Lines: 2 Message-ID: <5pbqef$4g6$2@usenet85.supernews.com> NNTP-Posting-Host: 207.211.62.221 From owner-7tms_r@net.bio.net Mon Jun 30 23:00:00 1997 Path: biosci!pravda.ucr.edu!newsfeed2.aimnet.com!europa.clark.net!dispatch.news.demon.net!demon!delos.dra.hmg.gb!server1.netnews.ja.net!server5.netnews.ja.net!server6.netnews.ja.net!server4.netnews.ja.net!server2.netnews.ja.net!news.qub.ac.uk!not-for-mail From: Andrew Wallace Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Moderate or unsubscribe?? Date: Tue, 01 Jul 1997 11:41:42 +0100 Organization: Queens University Belfast Message-ID: <33B8DEE4.32F4@qub.ac.uk.see.signature> References: Reply-To: a.wallace@qub.ac.uk.see.signature NNTP-Posting-Host: awall.bc.qub.ac.uk Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 3.0 (Macintosh; I; PPC) Lines: 31 David A. Schooley wrote: > > I thought a decision had been made to moderate communications about > a month ago. Since then, it seems the frequency of unsolicited mail has > only increased, until it greatly exceeds the scientific communications. > I have already seen a colleague from another department here > unsubscribe. Is moderation really going to happen? > > David A. Schooley > Dept. of Biochemistry/330 > Univ. of Nevada > Reno, NV 89557 > schooley@med.unr.edu > tel: (702) 784-4136; fax (702) 784-1419 See the message from the BioSci staff on the bionet.general newsgroup about moderation. BioSci have just moved their computers about 50 miles across the Bay area of San Francisco to Stanford without hardly missing a beat. I bet that most of you didn't even notice, the mail and newsgroups just kept working as usual. There are a lot of newsgroups going into moderation right now as a result of the spam flood, so I imagine the support staff are run off their feet. Please be patient and give them a chance. Andrew -- - note antispam feature in return address. My real address is: ================================================================== Andrew Wallace,Ph.D., Queens University Belfast, N. Ireland (UK) a.wallace@qub.ac.uk http://web.qub.ac.uk/bb/awpage/wallace.html ================================================================== From owner-7tms_r@net.bio.net Mon Jun 30 23:00:00 1997 Path: biosci!UMICH.EDU!him From: him@UMICH.EDU (Henry Mosberg) Newsgroups: bionet.molbio.proteins.7tms_r Subject: atomic resolution rhodopsin TM core model Date: 1 Jul 1997 12:17:52 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 53 Sender: daemon@net.bio.net Distribution: world Message-ID: <2.2.32.19970701192246.006c9e98@h.imap.itd.umich.edu> NNTP-Posting-Host: net.bio.net Atomic resolution models of the transmembrane domain of bovine rhodopsin and metarhodopsin II, which we (I.Pogozheva, A. Lomize, H. Mosberg) recently published in Biophysical J., v. 70, pp. 1963-1985, May 1997, have been deposited in the Protein Data Bank as files 1bok and 1boj. These models were to be released by July 1, but unfortunately, the files are still under "REVIEW" status at PDB. Anyone interested in receiving these coordinates in advance can do so by sending me a request via e-mail. The structure of the transmembrane 7-helix-bundle was calculated using an iterative distance geometry refinement with an evolving system of hydrogen bonds, formed by intramembrane polar side-chains in various proteins from the GPCR family and collectively applied as distance constraints. The "average" 7-helix-bundle structure thus obtained provides H-bonding of nearly all buried polar side-chains simultaneously in the 410 GPCRs considered. The "average" atomic model of the alpha-bundle has been tested by using it as a template to calculate the transmembrane domains of various GPCRs in addition to the rhodopsin/MetaII examples discussed in the Biophys.J. article. Each resulting GPCR model contains a binding pocket complementary in shape and polarity to its natural and synthetic ligands and is consistent with experimental data. At present, we have calculated and compared with experimental data the transmembrane domains of 28 different GPCRs, including red cone opsin, invertebrate (crayfish) rhodopsin, as well as opioid, chemokine, melatonin, purine, glycoprotein, and cationic amine receptors (for some of these, the results are still preliminary) and their complexes with many (mostly, relatively rigid) ligands The calculations of the individual receptor structures from the same "average" model are time-consuming, since they include determination of conformers for many side-chains which replace those found in the "average" model, some rearrangements of H-bond networks, small shifts of helices, appearance of Pro kinks, and so on. This work can not be done by using standard "modeling by homology" procedures; but requires distance geometry refinement with evolving constraints for each specific GPCR modeled. We have also developed a computational approach for modeling loops connecting the transmembrane helices, and have obtained preliminary results for the extracellular loops of opioid receptors and red opsin. We are interested in further experimental testing of the model. The most obvious test would be a direct fit with 6-7 A resolution electron microscopy 3D maps of frog rhodopsin, but unfortunately, the maps are unavailable at this time. The model can also be tested by incorporating disulfide bonds between spatially close cysteines, for example from Table 2 in our article in Biophysical Journal. Henry I. Mosberg e-mail: him@umich.edu Professor of Medicinal Chemistry Phone: (313)764-8117 College of Pharmacy FAX: (313)763-5633 University of Michigan Ann Arbor, MI 48109-1065 From owner-7tms_r@net.bio.net Mon Jun 30 23:00:00 1997 Path: biosci!ITSA.UCSF.EDU!barber From: barber@ITSA.UCSF.EDU (Diane Barber) Newsgroups: bionet.molbio.proteins.7tms_r Subject: postdoctoral position Date: 1 Jul 1997 11:42:28 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 15 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net An NIH-funded postdoctoral position at the University of California, San Francisco is available to investigate GTPase signaling networks and the actin cytoskeleton. Candidate should have experience studying signal transduction or the cytoskeleton. For lab focus, see JBC 1994 269:4721, JBC 1996 271:6152, JBC 1996 271:22281, JBC 1996 271:22604, PNAS 1996 93:12631. If interested, please send a cv, the names of three references to contact, and a statement of your research interests to: Diane L. Barber, PhD Box 0512 University of California San Francisco, CA 94143 From owner-7tms_r@net.bio.net Mon Jun 30 23:00:00 1997 Path: biosci!webtv.net!uunet!in1.uu.net!204.94.214.20!news.sgi.com!csulb.edu!gatech!howland.erols.net!newsfeed.nacamar.de!fu-berlin.de!news-ber1.dfn.de!news-lei1.dfn.de!news.uni-leipzig.de!mlucom4.urz.uni-halle.de!mlubio4.biochemtech.uni-halle.de!alex From: alex@mlubio4.biochemtech.uni-halle.de (Alexander Golbraikh) Newsgroups: bionet.molbio.proteins,bionet.molbio.proteins.7tms_r Subject: Cys-Cys in opr Date: 1 Jul 1997 14:47:19 GMT Organization: Martin-Luther-University, Dept. Biochem./Biotech. Lines: 17 Message-ID: <5pb59n$om7@mlucom4.urz.uni-halle.de> NNTP-Posting-Host: mlubio4.biochemtech.uni-halle.de Xref: biosci bionet.molbio.proteins:11114 bionet.molbio.proteins.7tms_r:1319 Dear netters: I suppose there must be disulphide bridges between Cys residues in N-termini of mu opioid receptor. Is it known if they really exist and between which Cysteins they are, say, in human oprm (there are four Cys's at positions 13,22,45 and 59)? Is there a bridge between Cysteins in N-terminus of kappa opioid receptor? Thank you in advance for answers. A.Golbraikh From owner-7tms_r@net.bio.net Mon Jun 30 23:00:00 1997 Path: biosci!pravda.ucr.edu!news.cs.ucr.edu!zdc!super.zippo.com!su-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!cam-news-hub1.bbnplanet.com!news.bbnplanet.com!news-xfer.netaxs.com!netnews.upenn.edu!news.tju.edu!not-for-mail From: "Dr. Philip Wedegaertner" Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdoc Position Date: Tue, 01 Jul 1997 08:08:46 -0400 Organization: Kimmel Cancer Institute, TJU Lines: 38 Message-ID: <33B8F34E.4568@lac.jci.tju.edu> Reply-To: P_Wedegaertner@lac.jci.tju.edu NNTP-Posting-Host: wedegaertner1.jci.tju.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit X-Mailer: Mozilla 2.0 (Macintosh; I; PPC) A postdoctoral position in the Kimmel Cancer Institute at Thomas Jefferson University is available in September 1997 to study cellular regulation of heterotrimeric G proteins with emphasis on mechanisms of lipid modifications and subcellular targeting [Mol. Biol. Cell 7: 1225 (1996); JBC 270: 503 (1995); Cell 77: 1063 (1994); JBC 268: 25001 (1993)]. The laboratory is interested in defining the underlying mechanisms of palmitoylation/depalmitoylation to determine how such covalent lipid modifications contribute to targeting of peripheral membrane proteins (e.g., signaling G proteins) to different membrane environments. Candidate must have background in molecular biology, biochemistry and/or cell biology and have a strong interest in signal transduction. The Kimmel Cancer Institute (http://www.jci.tju.edu) is located in the new Bluemle Life Sciences Building. The modern well-equipped laboratories are supported by extensive core facilities, including DNA synthesis and sequencing, peptide synthesis and sequencing, and confocal microscopy facilities. A number of research labs concentrate on diverse aspects of cellular signaling, providing a stimulating intellectual environment and ample opportunity for collaboration. Other areas of signal transduction research in the Kimmel Cancer Institute include regulation of G protein-coupled receptors, mechanisms of receptor endocytosis, cell signaling by inositol phosphates, receptor tyrosine kinase and tyrosine phosphatase signaling pathways, and mechanisms of apoptosis. Send curriculum vitae, a brief statement of research interests, and names, addresses, and phone numbers of 3 references to: Philip Wedegaertner, Ph.D. Kimmel Cancer Institute Thomas Jefferson University 839 BLSB, 233 S. 10th St. Philadelphia, PA 19107-5541 Phone: (215) 503-3137 FAX: (215) 923-2117 E-Mail: P_Wedegaertner@lac.jci.tju.edu