From owner-7tms_r@net.bio.net Sun Nov 02 22:00:00 1997 Path: biosci!biosci!not-for-mail From: H.deJonge@stud.biol.ruu.nl (Hugo de Jonge) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Phosphatase inhibition by fluorides. Date: 3 Nov 1997 16:00:04 -0800 Organization: Academic Computer Centre Utrecht, (ACCU) Lines: 6 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <63loi4$q7k@net.bio.net> NNTP-Posting-Host: net.bio.net Who knows a good article about phosphatase inhibition by NaF/AlF4- (fluorides)? I have used it to stimulate G proteins in studies on phospholipid signal transduction, but am affraid that it does more than stimulating G proteins. A good article would help me a lot. Hugo. From owner-7tms_r@net.bio.net Sun Nov 02 22:00:00 1997 Path: biosci!biosci!not-for-mail From: bzheng@ucsd.edu (Bin Zheng) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Q: GPCR and yeast two hybrid Date: 3 Nov 1997 16:00:06 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 9 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <63loi6$q7u@net.bio.net> NNTP-Posting-Host: net.bio.net Hi, there, I am wondering whether it is possible to fish out GPCR ( G protein coupled receptor) by yeast two-hybrid system, or use GPCR as the bait to fish out something else. Does anybody know of these kinds of experiments? Thanks! Bin Zheng From owner-7tms_r@net.bio.net Mon Nov 03 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Cornelius Krasel Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: Q: GPCR and yeast two hybrid Date: 4 Nov 1997 07:01:29 -0800 Organization: University of Wuerzburg, Germany Lines: 34 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <63ndc9$7th@net.bio.net> References: <63loi6$q7u@net.bio.net> NNTP-Posting-Host: net.bio.net Bin Zheng wrote: > I am wondering whether it is possible to fish out GPCR ( G protein coupled > receptor) by yeast two-hybrid system, or use GPCR as the bait to fish out > something else. Does anybody know of these kinds of experiments? Have a look into @article{klein:97, author = {Uwe Klein and M. Teresa Ramirez and Brian K. Kobilka and Mark {von Zastrow}}, title = {A Novel Interaction between Adrenergic Receptors and the $\alpha$-Subunit of Eukaryotic Initiation Factor {2B}.}, journal = JBC, volume = 272, number = 31, pages = {19099--19102}, year = 1997 } - --Cornelius. [PS: If you want to post in more than one newsgroup, please don't post the same message several times, but instead put all the newsgroups into the Newsgroups: line. For example: Newsgroups: bionet.molbio.methds-reagnts,bionet.molbio.proteins,bionet.molbio.proteins.7tms_r This is called "crossposting" and saves disk space on news servers, because here the article in question is saved only once, whereas in the case of multiple postings, there is a saved copy for every post. Thank you.] - -- /* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */ /* D-97078 Wuerzburg, Germany email: phak004@rzbox.uni-wuerzburg.de SP4 */ /* "Science is the game we play with God to find out what His rules are." */ From owner-7tms_r@net.bio.net Mon Nov 03 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Henrik Dohlman Newsgroups: bionet.molbio.proteins.7tms_r Subject: Post-doc @ Yale - RGS proteins Date: 4 Nov 1997 13:43:20 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 23 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <63o4to$roc@net.bio.net> NNTP-Posting-Host: net.bio.net An NIH funded post-doctoral fellowship position will be available (Spring, 1998) to study G protein regulation by RGS proteins in yeast. The ideal candidate will have experience in protein biochemistry, but all individuals with a record of accomplishment in any system will be considered. Interested applicants should reply by Email to: Henrik G. Dohlman, PhD Department of Pharmacology Yale University School of Medicine henrik.dohlman@yale.edu Please enclose a current CV and the names of at least two references. Further information about the laboratory can be found at: http://info.med.yale.edu/bcmm/dohlman/DohlmanLab.html Henrik G. Dohlman, PhD Assistant Professor Department of Pharmacology Yale University, School of Medicine henrik.dohlman@yale.edu 203-737-2203 (-2290 FAX) From owner-7tms_r@net.bio.net Fri Nov 07 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Ilya Vakser Newsgroups: bionet.molbio.proteins.7tms_r Subject: Postdocs/Graduate Students in Protein Docking and Structure Prediction Date: 8 Nov 1997 08:06:09 -0800 Organization: Medical University of South Carolina Lines: 30 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <6422lh$gaa@net.bio.net> NNTP-Posting-Host: net.bio.net Applications are invited for postdoctoral and graduate student positions in my laboratory at the Medical University of South Carolina. The main subjects of the research are computational studies of fundamental principles of molecular recognition, docking methodology, structure prediction, including membrane proteins modeling, and applications to signal transduction pathways and other molecular systems. Candidates for a postdoctoral position should have a background in biochemical or physics/mathematics/computer science areas. Computer programming skills will be a plus. The Medical University of South Carolina at Charleston provides a challenging research environment in pharmacology and biochemistry with the emphasis on the development of structural studies. The positions offer a rare opportunity to enjoy fundamental research at the cutting edge of computer modeling in structural biology in a relaxing atmosphere of a charming semi-tropical ocean resort. Charleston is a cosmopolitan community with rich cultural life (theaters, concerts, art galleries), great food, and beautiful beaches. To apply send a letter and CV with names of at least 2 referees. Ilya A. Vakser Assistant Professor of Pharmacology Department of Cell and Molecular Pharmacology Medical University of South Carolina 171 Ashley Avenue Charleston, SC 29425 Email: vakseri@musc.edu, Phone:(803)792-2471, Fax:(803)792-2475 From owner-7tms_r@net.bio.net Tue Nov 11 22:00:00 1997 Path: biosci!biosci!not-for-mail From: BIOSCI Administrator Newsgroups: bionet.molbio.proteins.7tms_r Subject: BIOSCI/bionet miniFAQ & Fundraiser Date: 12 Nov 1997 06:08:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 233 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <64cd81$f5d@net.bio.net> NNTP-Posting-Host: net.bio.net (LAST REVISION: 30-JUL-95) This BIOSCI "miniFAQ" is designed to answer the questions that come up the *most frequently*. The main BIOSCI FAQ (Frequently Asked Questions) is accessible on the World Wide Web at URL http://www.bio.net/. If you can not find an answer to your question in this or other documentation, the BIOSCI technical support staff answers e-mail queries sent to biosci-help@net.bio.net We can only answer questions about the use of the newsgroups and mailing lists. We unfortunately do not have the staff to do Internet information searches or answer scientific questions. Please post those to the appropriate BIOSCI/bionet newsgroups. Contents: -------- 0) BIOSCI NEEDS YOUR SUPPORT!! 1) Using the WWW to access the BIOSCI/bionet newsgroups. 2) What to do about "spams," i.e., junk mail, ads, etc. 3) Examples of subscribing and unsubscribing to the mailing lists. 4) The BIOSCI user address and research interest directory. 0) BIOSCI NEEDS YOUR SUPPORT!! - ------------------------------ BIOSCI's government funding has been expended, and we are now operating solely from advertising revenue that we have raised from our Web site at http://www.bio.net/. We need just a few minutes of your time to help us serve you. You can do two important things which will take very little time for you individually and will immensely help us continue to help you. First, please use our WWW system at http://www.bio.net/ to access the archives. You can post or reply to messages via your Web browser as described in item #1 below. Your usage helps attract sponsors. If you contact any of our sponsors, please be sure to thank them for supporting BIOSCI. It is critical for them to get this feedback if they are to continue their sponsorship for the long term. Second, if you work for a company or organization that provides products or services of interest to the biology community, please pass this message on to your marketing or marketing communications department or other appropriate group. Please ask them to help support BIOSCI by sponsoring our Web site and explain the uses and benefits of the system to the biology community. If they are interested, they can then contact us for further information at our tech support address, biosci-help@net.bio.net. 1) Using the WWW to access the BIOSCI/bionet newsgroups. - -------------------------------------------------------- As of 10 December 1995, all BIOSCI/bionet full newsgroups are accessible through the World Wide Web (WWW) at URL http://www.bio.net. One can read and reply publicly or privately to both recent postings and archived messages through one's Web browser if it is configured properly to send e-mail. Each newsgroup is equipped with its own WAIS index. The main BIOSCI home page also has access to the BIO-JOURNALS Table of Contents database WAIS index and the BIOSCI user address database described in another item further below. 2) What to do about "spams," i.e., junk mail, ads, etc. - ------------------------------------------------------- BIOSCI is a set of parallel USENET newsgroups (the "bionet" groups), mailing lists, and a hypermail archive at URL http://www.bio.net/. The same postings are distributed on all media (except for a small number of mailing-list-only groups at net.bio.net). Unfortunately it is becoming a despicable practice on the Internet (by a few people out to make a fast buck) to do automated mass postings to thousands of newsgroups and mailing lists. These attempts to grab free advertising are refered to as "spams" in the usual, somewhat boneheaded, net terminology. USENET is more susceptible to this practice, and many spams originate on the USENET groups and then are passed on to the mailing lists. However, spammers also get lists of mailing addresses and hit these too, so neither medium is immune. What should you do personally if you get junk mail? - --------------------------------------------------- Just delete it and move on without reading it further. Filing a protest is becoming increasingly useless because spammers are often disguising the addresses where the messages are sent from. Unless you really understand Internet mail systems, your attempt at protest by sending replies to the message will often end up being sent to the address of an innocent person that the spammer is victimizing. What can BIOSCI/bionet do to protect its newsgroups? - ---------------------------------------------------- The only solution currently available is to moderate the newsgroup. If this newsgroup is already moderated, then you are in good shape. Moderation protects the USENET distribution from about 95% of the spams that are being sent to date and protects the mailing lists completely. Moderation means, however, that someone has to take the time to review each message before it goes out. We have set up software here that simply allows the moderator to forward to an address at net.bio.net messages that (s)he wishes to have distributed. This takes no more time than that needed to read the message and pass it on, say about 1 min. per message. Most newsgroups currently have a discussion leader who is responsible for their newsgroup. The discussions leaders and their e-mail addresses are listed in the BIOSCI Information Sheet which is available on the Web at http://www.bio.net/. If a newsgroup is being hit with too many junk postings, please contact the discussion leader for that group and see if there is interest in moderating the group. Please do not assume that by simply posting a complaint to the newsgroup itself, anyone on the BIOSCI staff will act on your complaint. With close to 100 newsgroups to run, the BIOSCI staff has to rely on the discussion leaders of each newsgroup to report problems directly to us at biosci-help@net.bio.net. We will moderate any of our newsgroups if the discussion leader tells us that the readership of the group wishes to do so and if a moderator is willing to do the work. For most BIOSCI/bionet groups, this entails only a few minutes of work each day. Moderating a newsgroup will resolve probably 95% of the junk postings on the USENET distribution. Unfortunately there are easy ways for determined spammers to override the moderation mechanism on USENET, but we can protect our e-mail subscribers from unwanted postings if the newsgroup is moderated. You can also access our newsgroups over the WWW at URL http://www.bio.net. While this Web interface will not stop spammers from trying to post to the groups, this will give you yet another way, besides using USENET news, to keep the junk out of your personal mail files. For those of you with local USENET news systems, the Web interface will also give you faster access to new newsgroups and recent postings. 3) Examples of subscribing and unsubscribing to the mailing lists. - ------------------------------------------------------------------ PLEASE NOTE: The BIOSCI management does NOT act on subscription/unsubscription requests that are posted improperly to the newsgroups and mailing lists. People who do this only bother everyone on the lists to no avail. Please be sure to follow the proper procedures below. Gory details are in the BIOSCI Information sheets on the Web at http://www.bio.net. Below we give an example utilizing the METHODS-AND-REAGENTS list at both of our two BIOSCI sites: Users in the Americas and Pacific Rim countries who use the BIOSCI - ------------------------------------------------------------------ node at computer net.bio.net: - ---------------------------- A) Determine the "listname" which is the <=8 character mail address ^^^^^^^^^^^^^ for the group. These can be found in the BIOSCI Info. Sheet. For the METHODS-AND-REAGENTS group the mailing address is methods@net.bio.net. The listname is the portion of the address to the left of the @ sign, i.e., "methods". The listname is used with the "subscribe" and "unsubscribe" commands illustrated below. B) Mail all commands in the body of a mail message addressed to biosci-server@net.bio.net. Do NOT send commands to the newsgroup posting addresses! Leave the Subject: line blank, any text on it will be ignored. 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The USENET newsgroup names are listed in the BIOSCI Information sheet on the Web at http://www.bio.net/. For the METHODS-AND-REAGENTS list the USENET newsgroup name is bionet.molbio.methds-reagnts, thus the appropriate commands are sub bionet-news.bionet.molbio.methds-reagnts unsub bionet-news.bionet.molbio.methds-reagnts These commands are included in a message addressed to mxt@dl.ac.uk, NOT to the newsgroup mailing addresses. As usual, include the text in the body of the message as text on the Subject: line is ignored. To unsubscribe from all the lists at the UK node, use unsub bionet-news Please note that if the address in the list is different than the one in your mail message header, you will not be able to unsubscribe by this method. If you have problems, please mail biosci@daresbury.ac.uk. 4) The BIOSCI user address and research interest directory. - ----------------------------------------------------------- Please take this opportunity to add your name, address, and research interest information to the BIOSCI User Address Database if you have not already done so. You can fill out the address form directly through our Web page at URL http://www.bio.net/adrform.html. The address database is reindexed nightly for WWW access (the URL is http://www.bio.net/). If you are not directly on the Internet but can reach it by e-mail, please use our waismail server to access the user directory. waismail use is described above. You can also request a user address form by e-mail from biosci-help@net.bio.net. Please check your database entry from time-to-time to see if your address information is still up-to-date. Because of our limited personnel resources, we ask that you resubmit a *complete* form to revise your entry; we only replace complete entries and do not have resources to edit old forms. ------- End of forwarded message ------- From owner-7tms_r@net.bio.net Thu Nov 13 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Tracy Nero Newsgroups: bionet.molbio.proteins.7tms_r Subject: looking for the cloned rat beta-1-adrenoceptor Date: 14 Nov 1997 07:34:04 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 18 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <64hr1c$ag1@net.bio.net> NNTP-Posting-Host: net.bio.net Dear List Members, Can anyone tell me whether the rat beta-1-adrenoceptor clone is readily available, and if so which cell lines has it been transfected into? cheers Tracy Tracy Nero, PhD Clinical Pharmacology and Therapeutics Unit, Dept. of Medicine, The University of Melbourne, Austin Campus, Austin and Repatriation Medical Centre, Heidelberg, 3084, Victoria, Australia tracy@austin.unimelb.edu.au Ph. 61 3 94965052 Fax 61 3 94593510 From owner-7tms_r@net.bio.net Fri Nov 14 22:00:00 1997 Path: biosci!biosci!not-for-mail From: newsmgr@merrimack.edu Newsgroups: bionet.molbio.proteins.7tms_r Subject: Position Open Date: 15 Nov 1997 06:17:35 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 16 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <64katv$2kc@net.bio.net> NNTP-Posting-Host: net.bio.net Relay-Version: ANU News - V6.2.0 06/23/97 OpenVMS AXP V6.2; site chasm Path: chasm!cam-news-feed2.bbnplanet.com!cam-news-hub1.bbnplanet.com!cpk-news-hub1.bbnplanet.com!news.bbnplanet.com!newsfeed.internetmci.com!152.163.199.19!portc03.blue.aol.com!audrey01.news.aol.com!not-for-mail Newsgroups: bionet.molbio.proteins.7tms_r Subject: Position Open Message-ID: <19971115023801.VAA21288@ladder01.news.aol.com> From: sdwolpe@aol.com (SDWOLPE) Date: 15 Nov 1997 02:38:27 GMT Organization: AOL http://www.aol.com Lines: 4 NNTP-Posting-Host: ladder01.news.aol.com X-Admin: news@aol.com I have a position open for someone with experience in cloning G-linked receptors. We are a development stage pharmaceutical company in the Washington DC area. If interested please contact me at SDWolpe@AOL.com. Thanks. From owner-7tms_r@net.bio.net Mon Nov 17 22:00:00 1997 Path: biosci!biosci!not-for-mail From: nevek@teleport.com (Kim Neve) Newsgroups: bionet.molbio.proteins.7tms_r Subject: Re: looking for the cloned rat beta-1-adrenoceptor Date: 18 Nov 1997 06:59:18 -0800 Organization: Oregon Health Sciences University Lines: 12 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <64sag6$pfk@net.bio.net> References: <64hr1c$ag1@net.bio.net> NNTP-Posting-Host: net.bio.net Tracy Nero wrote: >Can anyone tell me whether the rat beta-1-adrenoceptor >clone is readily available, and if so which cell lines >has it been transfected into? Curt Machida has it at the Oregon Regional Primate Research Center, 505 NW 185th Ave., Beaverton, OR 97006, and he expressed it in L fibroblasts. I would guess that it is fairly readily available. It is genbank accession number J05561. There are other sequences in the database for rat beta-1, including D00634. Kim From owner-7tms_r@net.bio.net Wed Nov 19 22:00:00 1997 Path: biosci!biosci!not-for-mail From: daemon@net.bio.net Newsgroups: bionet.molbio.proteins.7tms_r Subject: post-doctoral position in signal transduction / France Date: 20 Nov 1997 06:19:23 -0800 Organization: CEA Lines: 52 Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <651gtb$3sh@net.bio.net> NNTP-Posting-Host: net.bio.net A post-doctoral position is available in Grenoble, France, to invest= igate a novel cellular signaling pathway that uses the ubiquitous but still mysterious serine/threonine kinase, CK2a (see H=E9rich=E9 et al., Sci= ence 276, 952, 1997). =20 Topics to be addressed will be the regulation of CK2a by mitogen and neurotrophin receptor-stimulated kinases; the regulation of protein phosphatase 2A (PP2A) by CK2a; or the role of CK2a in neuronal signal= ing. The Department of Structural and Molecular Biology is run by the Fren= ch research agencies, CEA, CNRS and INSERM. The Department has state-of-the-art facilities for molecular- and cell-biological work, including e. g. transgenic mouse lab, microinjection, Biacore, an on-= site contractor for automated DNA sequencing, in-house protein analysis by= 2d gel electrophoresis / mass spectrometry, and protein modeling. Grenoble has a large, international scientific and technical communit= y. Day care for young children is normally no problem. The city is locat= ed in a valley surrounded by the French Alps, with an international airport= 90 km away; Switzerland, Italy, Southern France and Paris are 2-3 hours = away by car or high-speed train. Applicants should have a PhD degree in an area related to molecular a= nd cellular biology, and be familiar with directed mutagenesis, protein expression and analysis, immunological assays and cell culture techni= ques. Phosphorylation site mapping and neuronal cell culture are a plus. It= is expected that candidates try to secure their own funding through one = of the available fellowship schemes; however, if needed, the Department= will be able to pay for the selected applicant=B9s salary. Potentially int= erested individuals are encouraged to send a.s.a.p. a CV and the names of two references to:=20 Dr. Yves Goldberg DBMS-BRCE 17 rue des Martyrs, F-38054 Grenoble, France Phone:+33 476 88 52 86 =20 Fax: +33 476 88 50 58 =20 E-mail: Yves@borgne.ceng.cea.fr From owner-7tms_r@net.bio.net Wed Nov 26 22:00:00 1997 Path: biosci!biosci!not-for-mail From: Clara Nahmias Newsgroups: bionet.molbio.proteins.7tms_r Subject: post-doc position Date: 27 Nov 1997 06:48:05 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Approved: lfk@gcrdb.uthscsa.edu Distribution: world Message-ID: <65k175$7ub@net.bio.net> NNTP-Posting-Host: net.bio.net A post-doctoral fellow position is available to join a CNRS research group of 5 investigators in the Department of Molecular Pharmacology at the Institute Cochin for Molecular Genetics in Paris, France. The project is to investigate inhibitory signalling pathways associated with angiotensin II AT2 receptors, functional coupling to tyrosine phosphatases, and negative cross-talk between GPCRs and tyrosine kinase receptors. =46or published information, consult: Nahmias and Strosberg, TiPS,vol 16, p223-225 (1995) and Bedecs et al., Biochem. J. vol 325, p449-454 (1997). Experience in receptor function or cell signalling would be advantageous but not essential. Expertise in cell culture, biochemistry or molecular biology is desirable. Applicants should provide a C.V and the names of two references to Dr. Clara Nahmias : CNRS UPR0415 Institut Cochin de G=E9n=E9tique Mol=E9culaire 22, rue Mechain 75014 Paris France e-mail : nahmias@cochin.inserm.fr Tel : 00 33 1 40 51 64 10 =46ax : 00 33 1 40 51 72 10