From wormlab from hawaii.edu Mon Mar 2 15:00:52 2009 From: wormlab from hawaii.edu (wormlab) Date: Mon Mar 2 15:11:56 2009 Subject: [Annelida] Working with Formalized Tissue...Help!! Message-ID: <49AC3AF4.4010407@hawaii.edu> Hi All, I’m looking for help getting DNA out of formalized worms. I’ve been trying to do DNA extractions using the Qiagen DNeasy Kit and I’m not getting anything. I’ve tried bathing the samples in two 15 minute washes of room temperature PBS before extraction, but that hasn’t worked either. If anyone has worked with formalized worms and has any advice, your help is greatly appreciated. Thanks! Emily Krause Zoology Department University of Hawaii Honolulu, HI 96822 (808) 956-5794 From lena.kupriyanova from gmail.com Mon Mar 2 19:03:19 2009 From: lena.kupriyanova from gmail.com (Elena Kupriyanova) Date: Mon Mar 2 19:28:00 2009 Subject: [Annelida] Working with Formalized Tissue...Help!! In-Reply-To: <49AC3AF4.4010407@hawaii.edu> References: <49AC3AF4.4010407@hawaii.edu> Message-ID: <49AC73C7.8090706@gmail.com> Dear Emily, It is not easy to say the least, so DNeasy kit clearly would not help. See, for example http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VGB-4D3W313-3&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=81f2530ea398b346e41433df5a24323b Boyle, E.E. Zardus J.D.,^ Chase M.R. Etter R.J. and Rex M.A.^ 2004. Strategies for molecular genetic studies of preserved deep-sea macrofauna. Deep-sea Res. Part 1. Ocean Res Pap 51: 1319-1336. Abstract With the development of new methods to sequence DNA from preserved organisms, existing archival collections can be used to document the population genetic structure of deep-sea species. This has made possible the first direct inferences about patterns of evolutionary diversification in the soft-sediment macrofauna. Here we report protocols and success rates for amplifying and sequencing regions of the mitochondrial 16S rDNA, Cytochrome oxidase I (COI), and Cytochrome b (cytb) genes from formalin-fixed protobranch bivalves and gastropods, major components of the deep-sea benthos. DNA was extracted from 1532 individuals of 12 common bathyal and abyssal species that had been fixed in formalin and preserved in alcohol for up to 36 years. DNA was also extracted from 53 individuals that were dried upon collection, some of which were collected more than 100 years ago. The overall success rate for amplification by PCR was 44%, but this varied considerably among species, stations, and cruises. When DNA amplified, sequencing success was generally high, averaging 85% and ranging from 19% to 100%. The reliability of amplification and sequencing depend strongly on how samples are treated during collection and storage. Amplification success was similar among samples collected from the same station and samples collected on the same cruise. We provide recommendations on strategies for primer design, PCR, and sample selection to improve success rates for genetic analysis of preserved deep-sea organisms. The success rates from different collections, sampling stations, and cruises provide important guidance for selecting material for future genetic work on deep-sea collections examined here. wormlab wrote: > Hi All, > > I’m looking for help getting DNA out of formalized worms. I’ve been > trying to do DNA extractions using the Qiagen DNeasy Kit and I’m not > getting anything. I’ve tried bathing the samples in two 15 minute > washes of room temperature PBS before extraction, but that hasn’t > worked either. If anyone has worked with formalized worms and has any > advice, your help is greatly appreciated. > > Thanks! > > Emily Krause > Zoology Department > University of Hawaii > Honolulu, HI 96822 > (808) 956-5794 > > _______________________________________________ > Annelida mailing list > Post: Annelida@net.bio.net > Help/archive: http://www.bio.net/biomail/listinfo/annelida > Resources: http://www.annelida.net From matz from mail.utexas.edu Mon Mar 2 19:37:39 2009 From: matz from mail.utexas.edu (mikhail matz) Date: Mon Mar 2 19:40:51 2009 Subject: [Annelida] inking in alcyopids Message-ID: Dear colleagues, I am looking for references on inking in acyopids. This remarkable phenomenon seems to be common knowledge among blue-water divers, but proves to be surprisingly difficult to trace in the literature. I am now writing a review about animal fluorescence, and want to see if anybody described what I observed - fluorescent ink in some alcyopids (would look pale yellow in a collection jar in daylight). If not that, I would tremendously appreciate references to any kind of defensive inking in these cute worms. best, Misha Mikhail V. Matz University of Texas at Austin Integrative Biology Section 1 University station C0930 Austin, TX 78712 phone 512-992-8086 cell, 512-475-6424 lab fax 512-471-3878 web http://www.bio.utexas.edu/research/matz_lab From g.read from niwa.co.nz Mon Mar 2 20:26:26 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Mon Mar 2 20:30:47 2009 Subject: [Annelida] inking in alciopids In-Reply-To: References: Message-ID: <49AD3E11.8045.00D5.0@niwa.co.nz> Hi Mikhail, I didn't know of this ability. But as it is in Google's database you will have seen the 1975 reference below. I mention it for others as a starter point for any earlier references. Hamner, W.M.; Madin, L.P.; Alldredge, A.L.; Gilmer, R.W.; Hamner, P.P. (1975). Underwater observations of gelatinous zooplankton: sampling problems, feeding biology, and behavior. Limnology and Oceanography 20(6): 907-917. http://aslo.org/lo/toc/vol_20/issue_6/0907.pdf p.915 "several alciopid polychaetes that released reddish-brown streamers of pigment as they swam away" Tomopterid bioluminescence is apparently mentioned in Herring, P.J. 1987. Systematic distribution of bioluminescence in living organisms. J. Biolum. Chemilum. 1 :147-163. Geoff >>> On 3/03/2009 at 1:37 p.m., mikhail matz wrote: > Dear colleagues, > > I am looking for references on inking in acyopids. This remarkable > phenomenon seems to be common knowledge among blue-water divers, but > proves to be surprisingly difficult to trace in the literature. I am > now writing a review about animal fluorescence, and want to see if > anybody described what I observed - fluorescent ink in some alcyopids > (would look pale yellow in a collection jar in daylight). If not > that, I would tremendously appreciate references to any kind of > defensive inking in these cute worms. > > best, > > Misha > > Mikhail V. Matz > University of Texas at Austin > Integrative Biology Section > 1 University station C0930 > Austin, TX 78712 > phone 512-992-8086 cell, 512-475-6424 lab > fax 512-471-3878 > web http://www.bio.utexas.edu/research/matz_lab -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ About NIWA http://www.niwa.co.nz/about *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. From bryony from seasurvey.co.uk Tue Mar 3 05:35:45 2009 From: bryony from seasurvey.co.uk (Bryony Pearce) Date: Tue Mar 3 17:13:23 2009 Subject: [Annelida] Sabellaria Larvae ID Message-ID: <49AD0801.9070207@seasurvey.co.uk> Dear All, I am undertaking some research investigating the reproductive cycle of the polychaete Sabellaria spinulosa and as part of this I have been collecting larval samples offshore. We have identified a number of metatrocaphore larvae which we think may be /Sabellaria spinulosa/ but are having difficulty confirming the ID. Does anyone know of anyone with expertise in this field that might perhaps be able to provide expert verification? Unfortunately most of the scientists I know of that have worked with /S. spinulosa/ larvae are dead. Any help would be greatly appreciated. Best Regards Bryony -- Bryony Pearce BSc (Hons) Research Director Marine Ecological Surveys Limited 24a Monmouth Place Bath, BA1 2AY Tel: +44 (0)1225 442211 Fax: +44 (0)1225 444411 http://www.seasurvey.co.uk/ From ndnestro from mail.ru Tue Mar 3 10:28:22 2009 From: ndnestro from mail.ru (=?koi8-r?Q?=EE=C1=D4=C1=CC=C9=D1_=E4=CE=C5=D3=D4=D2=CF=D7=D3=CB=C1=D1?=) Date: Tue Mar 3 17:14:22 2009 Subject: [Annelida] Nephys phyllocirra description Message-ID: Dear colleagues! I think you know my with Igor Jirkov paper in Nephyidae published in Jirkov (2001, http://narod.ru/disk/3269647000/Jirkov%202001%20(copy%203).pdf.html). Now I expand the region and so need the description of Nephtys phyllocirra Ehlers, E. 1887 (Report on the annelids of the dredging expedition of the U.S. coast survey steamer Blake. Memoirs of the Museum of Comparative Zoology at Harvard College, 15: Viand 335pp.) Unfortunately, our libraries haven't this source. Can some body help? ions? I would be VERY happy! Wormly Nataly Ph.D. Nataliya Yu. Dnestrovskaya Scientific researcher Hydrobiology dept. Faculty of Biology Lomonosov Moscow State University Moscow Russia From jdavenport from terraenv.com Tue Mar 3 07:55:28 2009 From: jdavenport from terraenv.com (Jennifer Davenport) Date: Tue Mar 3 17:15:28 2009 Subject: [Annelida] reprint request Message-ID: <455A25B8138FA04C8DF76A1AFF411BF761551B@server01.terraenv.local> Hello all, I'm working on a long-term monitoring program of the benthos of the Tampa Bay area and would greatly appreciate some help in locating a reprint to aid in my identifications. I have tried to request this particular article through interlibrary loan, but was unsuccessful. I tried to contact the author via the email address on the article, but that didn't work either. Does anyone have this reprint available and could you please email it to me? Thanks so much for the assistance! Nygren, A. 2004. Revision of the Autolytinae (Syllidae: Polychaeta). Zootaxa 680: 1-314. Jennifer Davenport Terra Environmental Services, Inc. 101 16th Avenue South, Suite 4 St. Petersburg, FL 33701 jdavenport@terraenv.com Office: (727) 565-4661 Cell: (727) 967-8450 Fax: (727) 565-4663 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20090303/d195a690/attachment.html From pljuscheva from mail.ru Tue Mar 3 12:58:56 2009 From: pljuscheva from mail.ru (=?koi8-r?Q?=ED=C1=DB=C1_=F0=CC=C0=DD=C5=D7=C1?=) Date: Tue Mar 3 17:16:32 2009 Subject: =?koi8-r?Q?Re=3A_[Annelida]_inking_in_alciopids?= Message-ID: Hi Misha, >From zoological point of view up to now the best review on Bioluminescence is still Harvey, 1952. HARVEY, E. N., 1952. Bioluminescence. Academic. Press,. New York According to my observation, in polychaetes bioluminescence is usually connected with fluorescence and those zones that produce bioluminescence usually become fluorescent. I never worked with alcyopids, but in cvase oh Chaetopterus and Mesochaetopterus it seems that there are two sources of fluorescence, epithelial cells and mucus, that produced by worm. And it seems that mucus fluorescence is due to symbiotic bacteria and in epithelial cells due to chemiluminescent-fluorescent proteins. Cheers, Masha Masha Plyuscheva, PhD Recearch Fellow Center for Advanced Studies (CEAB, CSIC) Acc Cala St. Francesc 14 17300 Blanes (Girona) Catalunia (Spain) Ph: + 34 972 336 101 Fax: + 34 972 337 806 From hermans from rockisland.com Tue Mar 3 19:29:46 2009 From: hermans from rockisland.com (Colin Hermans) Date: Tue Mar 3 19:33:56 2009 Subject: [Annelida] inking in alciopids In-Reply-To: <49AD3E11.8045.00D5.0@niwa.co.nz> References: <49AD3E11.8045.00D5.0@niwa.co.nz> Message-ID: <18650D93-EE28-4CC1-82A2-236D5EF4F977@rockisland.com> Dear Mikhail and Geoff: I think it is an outrageous coincidence that alciopids and cephalopods have evolved eyes that are so similar in structure right down to the ultrastructural level (Hermans, C.O. and R.M. Eakin (l974) Fine structure of the eyes of an alciopid polychaete, Vanadis tagensis. Zeit. Morph. Tiere. 79:245-267). Now on top of that, both groups have evolved inking. - Do alciopids really ink? What other group of aquatic animals ink? Is their a connection between great vision, and the evolution of inking? Like the shedding of tails by certain lizards, inking must be of value to the prey of a visual predator. The complexity of the eyes of alciopids suggests that they may be visual predators. - but then why would they ink? It would just mess up their own vision. Maybe visual deception, as practiced by cephalopod, and perhaps, by alciopids, could be the link between inking in the two groups? Just a thought, with best regards, Colin On Mar 2, 2009, at 5:26 PM, Geoff Read wrote: > Hi Mikhail, > > I didn't know of this ability. But as it is in Google's database > you will have seen the 1975 reference below. I mention it for > others as a starter point for any earlier references. > > Hamner, W.M.; Madin, L.P.; Alldredge, A.L.; Gilmer, R.W.; Hamner, > P.P. (1975). Underwater observations of gelatinous zooplankton: > sampling problems, feeding biology, and behavior. Limnology and > Oceanography 20(6): 907-917. http://aslo.org/lo/toc/vol_20/ > issue_6/0907.pdf > > p.915 "several alciopid polychaetes that released reddish-brown > streamers of pigment as they swam away" > > Tomopterid bioluminescence is apparently mentioned in Herring, P.J. > 1987. Systematic distribution of bioluminescence in living > organisms. J. > Biolum. Chemilum. 1 :147-163. > > > Geoff > > >>>> On 3/03/2009 at 1:37 p.m., mikhail matz >>>> wrote: >> Dear colleagues, >> >> I am looking for references on inking in acyopids. This remarkable >> phenomenon seems to be common knowledge among blue-water divers, but >> proves to be surprisingly difficult to trace in the literature. I am >> now writing a review about animal fluorescence, and want to see if >> anybody described what I observed - fluorescent ink in some alcyopids >> (would look pale yellow in a collection jar in daylight). If not >> that, I would tremendously appreciate references to any kind of >> defensive inking in these cute worms. >> >> best, >> >> Misha >> >> Mikhail V. Matz >> University of Texas at Austin >> Integrative Biology Section >> 1 University station C0930 >> Austin, TX 78712 >> phone 512-992-8086 cell, 512-475-6424 lab >> fax 512-471-3878 >> web http://www.bio.utexas.edu/research/matz_lab > > -- > > Geoff Read > http://www.annelida.net/ > http://www.niwascience.co.nz/ncabb/ > About NIWA http://www.niwa.co.nz/about > *************************** > > > NIWA is the trading name of the National Institute of Water & > Atmospheric Research Ltd. > > _______________________________________________ > Annelida mailing list > Post: Annelida@net.bio.net > Help/archive: http://www.bio.net/biomail/listinfo/annelida > Resources: http://www.annelida.net From eegonzales from berkeley.edu Tue Mar 3 19:55:20 2009 From: eegonzales from berkeley.edu (Eric Gonzales) Date: Tue Mar 3 20:00:22 2009 Subject: [Annelida] inking in alciopids In-Reply-To: <18650D93-EE28-4CC1-82A2-236D5EF4F977@rockisland.com> References: <49AD3E11.8045.00D5.0@niwa.co.nz> <18650D93-EE28-4CC1-82A2-236D5EF4F977@rockisland.com> Message-ID: <3FCA5FE9-C63C-46FA-846A-8E52C6FF1DB8@berkeley.edu> Regarding Colin Hermans intriguing idea: Sea hares are known to ink - http://home.earthlink.net/~huskertomkat/hare.html - but are not known for their great vision. Definitely want to learn more about alciopid ink & eyes. Best, Eric :::::::::::::::::::::::::::::::::::::::::::::::::::::: Eric Gonzales PhD Postdoc - Rokhsar Laboratory University of California at Berkeley Molecular and Cell Biology 543 Life Sciences Addition Berkeley CA 94720 Phone 510 643 9944 Cell 415 601 4923 Email eegonzales@berkeley.edu :::::::::::::::::::::::::::::::::::::::::::::::::::::: On Mar 3, 2009, at 4:29 PM, Colin Hermans wrote: > Dear Mikhail and Geoff: > > I think it is an outrageous coincidence that alciopids and > cephalopods have evolved eyes that are so similar in structure right > down to the ultrastructural level (Hermans, C.O. and R.M. Eakin > (l974) Fine structure of the eyes of an alciopid polychaete, Vanadis > tagensis. Zeit. Morph. Tiere. 79:245-267). Now on top of that, > both groups have evolved inking. - Do alciopids really ink? > > What other group of aquatic animals ink? Is their a connection > between great vision, and the evolution of inking? Like the > shedding of tails by certain lizards, inking must be of value to the > prey of a visual predator. The complexity of the eyes of alciopids > suggests that they may be visual predators. - but then why would > they ink? It would just mess up their own vision. Maybe visual > deception, as practiced by cephalopod, and perhaps, by alciopids, > could be the link between inking in the two groups? > > Just a thought, with best regards, Colin > > On Mar 2, 2009, at 5:26 PM, Geoff Read wrote: > >> Hi Mikhail, >> >> I didn't know of this ability. But as it is in Google's database >> you will have seen the 1975 reference below. I mention it for >> others as a starter point for any earlier references. >> >> Hamner, W.M.; Madin, L.P.; Alldredge, A.L.; Gilmer, R.W.; Hamner, >> P.P. (1975). Underwater observations of gelatinous zooplankton: >> sampling problems, feeding biology, and behavior. Limnology and >> Oceanography 20(6): 907-917. http://aslo.org/lo/toc/vol_20/issue_6/0907.pdf >> >> p.915 "several alciopid polychaetes that released reddish-brown >> streamers of pigment as they swam away" >> >> Tomopterid bioluminescence is apparently mentioned in Herring, P.J. >> 1987. Systematic distribution of bioluminescence in living >> organisms. J. >> Biolum. Chemilum. 1 :147-163. >> >> >> Geoff >> >> >>>>> On 3/03/2009 at 1:37 p.m., mikhail matz >>>>> wrote: >>> Dear colleagues, >>> >>> I am looking for references on inking in acyopids. This remarkable >>> phenomenon seems to be common knowledge among blue-water divers, but >>> proves to be surprisingly difficult to trace in the literature. I am >>> now writing a review about animal fluorescence, and want to see if >>> anybody described what I observed - fluorescent ink in some >>> alcyopids >>> (would look pale yellow in a collection jar in daylight). If not >>> that, I would tremendously appreciate references to any kind of >>> defensive inking in these cute worms. >>> >>> best, >>> >>> Misha >>> >>> Mikhail V. Matz >>> University of Texas at Austin >>> Integrative Biology Section >>> 1 University station C0930 >>> Austin, TX 78712 >>> phone 512-992-8086 cell, 512-475-6424 lab >>> fax 512-471-3878 >>> web http://www.bio.utexas.edu/research/matz_lab >> >> -- >> >> Geoff Read >> http://www.annelida.net/ >> http://www.niwascience.co.nz/ncabb/ >> About NIWA http://www.niwa.co.nz/about >> *************************** >> >> >> NIWA is the trading name of the National Institute of Water & >> Atmospheric Research Ltd. >> >> _______________________________________________ >> Annelida mailing list >> Post: Annelida@net.bio.net >> Help/archive: http://www.bio.net/biomail/listinfo/annelida >> Resources: http://www.annelida.net > > _______________________________________________ > Annelida mailing list > Post: Annelida@net.bio.net > Help/archive: http://www.bio.net/biomail/listinfo/annelida > Resources: http://www.annelida.net -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20090303/ff26e493/attachment.html From g.read from niwa.co.nz Tue Mar 3 20:04:44 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Tue Mar 3 20:06:31 2009 Subject: [Annelida] Nephys phyllocirra description In-Reply-To: References: Message-ID: <49AE8A7B.8045.00D5.0@niwa.co.nz> I can supply at least the Nephtys phyllocirra description to Nataly, but if someone has digitised it already let me know. Unfortunately this particular volume (15) appears not to have been digitised by Biodiversity Heritage library, though most of the rest of the serial have been. I do have all the plates digitised from long ago, though not the text. Hope to put them online. Some are rather good. Geoff >>> On 4/03/2009 at 4:28 a.m., ??????? ???????????? wrote: > Dear colleagues! > I think you know my with Igor Jirkov paper in Nephyidae published in Jirkov > (2001, > http://narod.ru/disk/3269647000/Jirkov%202001%20(copy%203).pdf.html). Now I > expand the region and so need the description of Nephtys phyllocirra Ehlers, > E. 1887 (Report on the annelids of the dredging expedition of the U.S. coast > survey steamer Blake. Memoirs of the Museum of Comparative Zoology at Harvard > College, 15: Viand 335pp.) > > Unfortunately, our libraries haven't this source. Can some body help? ions? > I would be VERY happy! > Wormly Nataly > > > Ph.D. Nataliya Yu. Dnestrovskaya > Scientific researcher > Hydrobiology dept. Faculty of Biology > Lomonosov Moscow State University > Moscow > Russia > > _______________________________________________ > Annelida mailing list > Post: Annelida@net.bio.net > Help/archive: http://www.bio.net/biomail/listinfo/annelida > Resources: http://www.annelida.net -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ About NIWA http://www.niwa.co.nz/about *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. From g.read from niwa.co.nz Tue Mar 3 22:14:55 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Tue Mar 3 22:18:07 2009 Subject: [Annelida] Sabellaria Larvae ID In-Reply-To: <49AD0801.9070207@seasurvey.co.uk> References: <49AD0801.9070207@seasurvey.co.uk> Message-ID: <49AEA8FE.8045.00D5.0@niwa.co.nz> Hello Bryony, Not sure if this will be much help. First Michel Bhaud is very expert in id from plankton, but perhaps such a problem these days could be tackled molecularly? Is there any data in GenBank? However, noting David Kirtley thought there might be "more than one definable species" under the name 'spinulosa'. I imagine you have reviewed the lit specifically on development of the species (DP Wilson, et al), but anyway for general European plankton this paper is good if you have not seen it: Bhaud, M.; Cazaux, C. (1987). Description and identification of polychaete larvae, their implications in current biological problems. Oceanis. 13(6): 595-753. And I wonder if Gunnar Thorson covered that species (haven't got my copy handy) in his magnum opus? Thorson, G. (1946). Reproduction and Larval Development of Danish Marine Bottom Invertebrates, with Special Reference to the Planktonic Larvae in the Sound (Oresund). Meddlelser fra Kommissionen for Danmarks Fiskeri-og Havundersogelser,Serie Plankton 4(1): 1-523. Good luck, Geoff Read >>> On 3/03/2009 at 11:35 p.m., Bryony Pearce wrote: > Dear All, > > I am undertaking some research investigating the reproductive cycle of > the polychaete Sabellaria spinulosa and as part of this I have been > collecting larval samples offshore. We have identified a number of > metatrocaphore larvae which we think may be /Sabellaria spinulosa/ but > are having difficulty confirming the ID. Does anyone know of anyone with > expertise in this field that might perhaps be able to provide expert > verification? Unfortunately most of the scientists I know of that have > worked with /S. spinulosa/ larvae are dead. Any help would be greatly > appreciated. > > Best Regards > Bryony -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ About NIWA http://www.niwa.co.nz/about *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. From Stanislas.Dubois from ifremer.fr Wed Mar 4 03:50:01 2009 From: Stanislas.Dubois from ifremer.fr (Stanislas DUBOIS) Date: Wed Mar 4 03:58:46 2009 Subject: [Annelida] Sabellaria Larvae ID In-Reply-To: <49AEA8FE.8045.00D5.0@niwa.co.nz> References: <49AD0801.9070207@seasurvey.co.uk> <49AEA8FE.8045.00D5.0@niwa.co.nz> Message-ID: <49AE40B9.1030206@ifremer.fr> Hi, I'm not sure this will help but I spent some time sorting Sabellaria alveolata larvae out of plancton samples! You can have a look at Dubois, S., Comtet, T., Retiere, C., Thiebaut, E., 2007. Distribution and retention of Sabellaria alveolata larvae (Polychaeta : Sabellariidae) in the Bay of Mont-Saint-Michel, France. Marine Ecology-Progress Series 346, 243-254 I IDed the sabellariid larvae them with Cazaux: Cazaux, C., 1964. D?veloppement larvaire de Sabellaria alveolata (Linn?). Bulletin de l'Institut Oc?anographique de Monaco 62, 1-15. Cazaux, C., 1970. Recherches sur l'?cologie et le d?veloppement larvaire des Polych?tes de la r?gion d'Arcachon, p. 355. facult? des Sciences de l'Universit? de Bordeaux, Bordeaux. (this PhD thesis is in French) From my small experience, I can tell you that ID sabellariid larvae is easy but separating down to species is very difficult, not to say too difficult without molecular help! Cheers, Stan. Geoff Read a ?crit : > Hello Bryony, > > Not sure if this will be much help. First Michel Bhaud is very expert in id from plankton, but perhaps such a problem these days could be tackled molecularly? Is there any data in GenBank? However, noting David Kirtley thought there might be "more than one definable species" under the name 'spinulosa'. > > I imagine you have reviewed the lit specifically on development of the species (DP Wilson, et al), but anyway for general European plankton this paper is good if you have not seen it: > > Bhaud, M.; Cazaux, C. (1987). Description and identification of polychaete larvae, their implications in current biological problems. Oceanis. 13(6): 595-753. > > And I wonder if Gunnar Thorson covered that species (haven't got my copy handy) in his magnum opus? > > Thorson, G. (1946). Reproduction and Larval Development of Danish Marine Bottom Invertebrates, with Special Reference to the Planktonic Larvae in the Sound (Oresund). Meddlelser fra Kommissionen for Danmarks Fiskeri-og Havundersogelser,Serie Plankton 4(1): 1-523. > > Good luck, > > Geoff Read > > >>>> On 3/03/2009 at 11:35 p.m., Bryony Pearce wrote: >> Dear All, >> >> I am undertaking some research investigating the reproductive cycle of >> the polychaete Sabellaria spinulosa and as part of this I have been >> collecting larval samples offshore. We have identified a number of >> metatrocaphore larvae which we think may be /Sabellaria spinulosa/ but >> are having difficulty confirming the ID. Does anyone know of anyone with >> expertise in this field that might perhaps be able to provide expert >> verification? Unfortunately most of the scientists I know of that have >> worked with /S. spinulosa/ larvae are dead. Any help would be greatly >> appreciated. >> >> Best Regards >> Bryony > > > -- ______________________________________________________ Stanislas DUBOIS (PhD) Laboratoire DYNECO / Ecologie Benthique IFREMER - French Research Institute for Exploitation of the Sea Technopole de Brest-Iroise B.P. 70 - 29280 Plouzan? - FRANCE Tel: (33) +2 98 22 49 18 Mob: (33) +6 83 07 70 28 Fax: (33) +2 98 22 45 48 email: sdubois@ifremer.fr From g.read from niwa.co.nz Wed Mar 11 16:15:19 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Wed Mar 11 16:17:21 2009 Subject: [Annelida] Marine Macroinvertebrate Taxonomist Message-ID: <49B8E0B6.8045.00D5.0@niwa.co.nz> Hi all, UK consultancy, APEM Ltd, is advertising for staff for several positions, including this one: Marine Macroinvertebrate Taxonomist http://www.apemltd.co.uk/vacancieslab.aspx and others: http://www.apemltd.co.uk/vacanciesaes.aspx FYI Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ About NIWA http://www.niwa.co.nz/about *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. From msjoneser from gmail.com Fri Mar 13 10:15:00 2009 From: msjoneser from gmail.com (Scott Jones) Date: Fri Mar 13 15:42:44 2009 Subject: [Annelida] techniques for illustrations intended for publication Message-ID: <171f92f0903130815o2375b8ej6b6f8ec5892ab90a@mail.gmail.com> Hi! I would like to survey the list for the most common and/or most effective way for non-illustrators to generate illustrations of specimens for publication. Do most of you rely on the camera lucida? What about importing digital images into editing software such as Illustrator to create line drawings? Thanks! Scott Jones -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20090313/ce773170/attachment.html From kjosborn from ucsd.edu Fri Mar 13 16:48:06 2009 From: kjosborn from ucsd.edu (Karen J. Osborn) Date: Fri Mar 13 18:20:57 2009 Subject: [Annelida] techniques for illustrations intended for publication In-Reply-To: <171f92f0903130815o2375b8ej6b6f8ec5892ab90a@mail.gmail.com> References: <171f92f0903130815o2375b8ej6b6f8ec5892ab90a@mail.gmail.com> Message-ID: <2A8509D7-76D3-4D00-A7C4-8A26ADB85829@ucsd.edu> Hi Scott, The best way is with a camera lucida - this take practice and a little instruction. Then you can ink your drawings by hand using vellum and scan them into the computer or better yet, scan your pencil sketches into the computer and use them to make Adobe Illustrator line drawings with the pen (or pencil more complicated) tool. A drawing pad helps with this immensely. Once you have an Illustrator file you can resize, reorient, change line weight, change color, and compile figures endlessly - the flexibility makes it well worth the steep learning curve and cost of Illustrator. You can also do this from photos but the problem with photos is that you have a single, usually narrow focal plane which doesn't lend itself well to illustrations which you want to be a bit 3-D. The one thing that Illustrator line drawings can't do well is stippling but there are a couple ways to do it. Good photos often suffice and lack the interpretation by the illustrator that is inherent in line drawings. Be sure photos are well labeled and clear. I like the combination of a photo and line drawing interpretation next to it of it to map out what the viewer is looking at. Best of luck, Karen On Mar 13, 2009, at 8:15 AM, Scott Jones wrote: > Hi! > > I would like to survey the list for the most common and/or most > effective way for non-illustrators to generate illustrations of > specimens for publication. > > Do most of you rely on the camera lucida? > > What about importing digital images into editing software such as > Illustrator to create line drawings? > > Thanks! > > Scott Jones > > ~~~ Karen Osborn, Ph.D. UC President's Postdoctoral Fellow Scripps Institution of Oceanography University of California, San Diego 9500 Gilman Dr., La Jolla, CA 92093-0202 office 858-534-3873 laboratory 858-534-9941 fax 858-534-7313 kjosborn@ucsd.edu Direct Shipping address: SIO, UCSD 8750 Biological Grade Hubbs Hall #2135 La Jolla, CA 92037 ~~~ -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20090313/8392c59a/attachment.html From Michael.Reuscher from tamucc.edu Fri Mar 13 17:21:57 2009 From: Michael.Reuscher from tamucc.edu (Reuscher, Michael) Date: Fri Mar 13 18:21:50 2009 Subject: [Annelida] techniques for illustrations intended for publication Message-ID: <22E76B7D3944A84A878AC660C2F4A9DEABF9C5@Hermes.ad.tamucc.edu> Hi Scott, I do both, pencil drawing with camera lucida then scan the drawings and finally digitalize the drawings with a Wacom Intuos Drawing Tablet and Adobe Illustrator. Check Coleman (2003) about digital inking. It is also possible to use digital images although I have the experience that a pencil drawing is beneficial. Wacom has a new drawing board that has a display screen (Cintiq series), but this is much more expensive than the standard drawing tablets. best, Michael Michael Reuscher, Ph.D. Student (Marine Biology) Harte Research Institute for Gulf of Mexico Studies Texas A&M University - Corpus Christi 6300 Ocean Drive, Unit 5869 Corpus Christi, Texas 78412-5869 -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20090313/76ca66f5/attachment.html From Jason.D.Williams from hofstra.edu Sat Mar 14 10:27:18 2009 From: Jason.D.Williams from hofstra.edu (Jason Williams) Date: Sat Mar 14 16:00:48 2009 Subject: [Annelida] techniques for illustrations intended for publication Message-ID: <49BB9496020000940000F8B8@GW15.hofstra.edu> Hi Scott & others, I produced a quick guide for use of Adobe Illustrator in creating final line drawings for a workshop at the IPC meeting in Maine (the document is attached). An excellent, and much more comprehensive guide (but geared toward entomologists) can be downloaded from Ralph W. Holzenthal?s website: www.entomology.umn.edu/museum/links/coursefiles/ENT%205051/PDF%20documents/AI%20for%20Entomologists.pdf As indicated by others, of interest along these lines also are Coleman (2003, 2006), but unlike in arthropods, digital picture conversion to line drawings is difficult in polychaetes due to the narrow focal plane and the lack of contrast in specimens. Coleman, C.O., 2003. "Digital inking": how to make perfect line drawings on computers. Organisms, Diversity and Evolution, Electronic Supplement, http://senckenberg.de/odes/03-14.htm, 1?14. Coleman, C.O., 2006. Substituting time-consuming pencil drawings in arthropod taxonomy using stacks of digital photographs. Zootaxa 1360, 61-68. Best wishes, Jason Jason D. Williams, Ph.D. Biology Department Hofstra University Hempstead, NY 11549 Phone: 516-463-5524 Email: biojdw@hofstra.edu Website: http://people.hofstra.edu/jason_d_williams/ >>> Scott Jones 03/13/09 4:55 PM >>> Hi! I would like to survey the list for the most common and/or most effective way for non-illustrators to generate illustrations of specimens for publication. Do most of you rely on the camera lucida? What about importing digital images into editing software such as Illustrator to create line drawings? Thanks! Scott Jones -------------- next part -------------- A non-text attachment was scrubbed... Name: WilliamsIllustratorWorkshop.pdf Type: application/pdf Size: 167515 bytes Desc: not available Url : http://www.bio.net/bionet/mm/annelida/attachments/20090314/c14be03b/WilliamsIllustratorWorkshop.pdf From lena.kupriyanova from gmail.com Tue Mar 17 03:32:46 2009 From: lena.kupriyanova from gmail.com (Elena Kupriyanova) Date: Tue Mar 17 13:58:48 2009 Subject: [Annelida] A new monograph on serpulid taxonomy Message-ID: <49BF602E.1090602@gmail.com> Dear friends and colleagues, Harry ten Hove and I are happy to inform you all that our monograph on the current state of serpulid taxonomy has been published by Zootaxa: Hove ten, H.A., Kupriyanova, E. K. 2009. Taxonomy of Serpulidae (Annelida, Polychaeta): the state of affairs. /Zootaxa /2036: 1-126.** It is an Open Access paper, so you can download it freely from http://www.mapress.com/zootaxa/2009/f/zt02036p126.pdf I also would like to use this occasion to ask you for samples of ethanol-preserved serpulid material. Any serpulids will be highly appreciated, but samples from deep-water water and hydrothermal locations would make me especially happy. Wormly, Lena -- Dr Elena Kupriyanova Faculty of Education and Human Sciences Yokohama National University Tokiwadai, Hodogaya, Yokohama 240-8501, Japan Tel and Fax +81 45 339 3408 e-mail: lena.kupriyanova@gmail.com elenak@ynu.ac.jp From g.read from niwa.co.nz Fri Mar 20 01:16:12 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Fri Mar 20 01:21:52 2009 Subject: [Annelida] Opportunity window to worm into Royal Society, UK journals Message-ID: <49C3EB7C.8045.00D5.0@niwa.co.nz> FYI, or a reminder that: "Royal Society Publishing is pleased to announce that, as from 18 February 2009, our online journal content will be hosted on the new HighWire H2O platform, where all articles - from 1665 to present - will be completely FREE to access until 31 March 2009." http://royalsocietypublishing.org Heh. A remarkable resource. If you search for worm titles from way back in 1665 - there _are_ actually some there .... Observations about Shining Worms in Oysters. Phil. Trans. 1665 1:203-206. An Extract of a Letter, Written from Holland, about Preserving of Ships from Being Worm-Eaten. Phil. Trans. 1665 1:190-191. A Relation of a Kind of Worms, That Eat Out Stones. Phil. Trans. 1665 1:321-323. Or from 30 years later you can see one of the first descriptions of an aphroditid, including observation of the interference colours of the chaetae, which are "... of a most delicate changeable Red and Green Colour and of so sparkling a vivid Lustre, that nothing of this kind could shew more Beautiful." The worm was found in a fish gut, rather than a dredge. Molyneux, T. (1695). Account of a Not Yet Described Scolopendra Marina. Philosophical Transactions 19(215-235): 405-412. Or if you prefer current times you can read about worm appendage Fibonacci patterns, about a pyritized polychaete from the Devonian, and about Osedax bone worms. Etc. Please share if you turn up other historical worm gems. Geoff -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ About NIWA http://www.niwa.co.nz/about *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. From a.glover from nhm.ac.uk Fri Mar 20 07:36:54 2009 From: a.glover from nhm.ac.uk (Adrian Glover) Date: Fri Mar 20 14:52:41 2009 Subject: [Annelida] polychaetes.info Message-ID: <49F4F4B5-0D01-4536-976B-B6E07C8B488E@nhm.ac.uk> Dear colleagues, I have created a website at http://polychaetes.info which some of you may find useful. It is a 'Web 2.0' style site created using the system developed by the European Distributed Institute of Taxonomy (EDIT). It started as something of an experiment, but I now believe it may be of genuine use to other polychaete workers. The site was a project outcome of a workshop on abyssal polychaetes held at the NHM in London. It was felt that a modern data-sharing website was required so that workers could share informal taxonomic descriptions and images. A major problem that we highlighted was the lack of revisionary taxonomy, and the lack of public availability for taxonomic data produced by many large-scale abyssal biodiversity studies that have been undertaken in recent years. This includes actual names, and a lot of 'informal' descriptions (sp. A, B, C etc). Whilst there are some efforts underway to formally describe these species, the resources allocated to taxonomy have been so small (compared with the costs of the collecting) that rather little has been published. The site I have created allows users to log in, upload their own species records, descriptions and images using forms, comment on (and edit) other data, geo-reference their data on a google map module, search for records and descriptions, and so forth. Anybody can view the data, only registered users can add data and edit what is there. For example, we are using it to share taxonomic data from recent biodiversity surveys of deep-sea canyons, the abyssal Pacific and the Antarctic. We hope that this will encourage future revisionary taxonomy, as well as better quality control of our own identifications. I have also uploaded about 4000 records of deep-sea polychaetes from a CeDAMar / CoML sponsored databasing exercise. Everything is geo- referenced on the map, but most of these records did not come with scanned images or drawings unfortunately. I am aware that there are rather a lot of web initiatives at the moment (e.g EoL, WORMS, GBIF etc). Our small site is obviously not intended as any sort of competition. Rather, I see small 'user- generated' online initiatives like this being useful in channeling data to the larger global initiatives such as EoL, which lack data. I will be interested to hear any feedback. If you would like to upload any of your own data, there is a link on the home screen to apply for a user account. We are not restricting it to just deep-sea data. Adrian Glover _________________________________________ Dr Adrian Glover Zoology Department The Natural History Museum Cromwell Rd, London SW7 5BD, UK _________________________________________ +44 (0)20 7942 5056 (office) +44 (0)77 666 48 440 (mobile) a.glover@nhm.ac.uk OR adrianglover@mac.com _________________________________________ http://www.nhm.ac.uk/research-curation/staff-directory/zoology/a-glover/ http://polychaetes.info From enteropneusta from gmail.com Mon Mar 23 11:21:25 2009 From: enteropneusta from gmail.com (=?ISO-8859-1?Q?Andr=E9_Souza_Santos?=) Date: Mon Mar 23 13:59:41 2009 Subject: [Annelida] Polychaeta larvae Message-ID: Dear colleges, I would like to know if somebody could send to me article about origin of nuchal organ in polychaeta larvae. I would like another help: I will start to make a SEM from some larvae and I appreciate a good clue about the better methodology. Thanks in advantage, Andr? -- Andr? Souza dos Santos Programa de P?s-Gradua??o em Ci?ncias Biol?gicas com ?rea de concentra??o em Zoologia Laborat?rio e Cole??o de Invertebrados Paulo Young Departamento de Sistem?tica e Ecologia Centro de Ci?ncias Exatas e da Natureza Universidade Federal da Para?ba Campus I, Cidade Universit?ria, Cep 58059-900 Jo?o Pessoa, Para?ba, Brazil. From g.read from niwa.co.nz Mon Mar 23 15:06:58 2009 From: g.read from niwa.co.nz (Geoff Read) Date: Mon Mar 23 15:08:48 2009 Subject: [Annelida] Polychaeta larvae In-Reply-To: References: Message-ID: <49C8A2B1.8045.00D5.0@niwa.co.nz> Possible starting points: Purschke, G. (2005). Sense organs in polychaetes (Annelida). Hydrobiologia 535(1): 53-78. http://www.springerlink.com/content/j7x2115024v07748/ Schlotzer-Schrehardt, U. (1986). Ultrastructural investigation of the nuchal organs of Pygospio elegans (Polychaeta). 1. Larval nuchal organs. Helgolaender Meeresuntersuchungen 40(4): 397-417,illustr. => Helgoland Marine Research http://www.springerlink.com/content/103796/ >>> On 24/03/2009 at 5:21 a.m., Andr? Souza Santos wrote: > Dear colleges, > > I would like to know if somebody could send to me article about origin > of nuchal organ in polychaeta larvae. > > I would like another help: I will start to make a SEM from some larvae > and I appreciate a good clue about the better methodology. > > Thanks in advantage, > > Andr? -- Geoff Read http://www.annelida.net/ http://www.niwascience.co.nz/ncabb/ About NIWA http://www.niwa.co.nz/about *************************** NIWA is the trading name of the National Institute of Water & Atmospheric Research Ltd. From Mahaffy from dordt.edu Mon Mar 30 14:37:16 2009 From: Mahaffy from dordt.edu (James Mahaffy) Date: Mon Mar 30 14:44:35 2009 Subject: [Annelida] Biggest earthworm Message-ID: <20090330193722.6957571123B@gray.dordt.edu> Folks, I am lecturing on annelids in Zoology and I decided to check the size of the largest worms. A number of places on the internet give 22 feet as the size of a record for "The South African Giant Earthworm grows to be up to 22 feet and is the largest earthworm known. The record-setting specimen was found beside a road in William's Town, South Africa, in 196&." This came from http://www.csmonitor.com/2005/0621/p18s02-hfks.html but I saw the same length quoted on a lot of sites. That almost sounds to be too big to me but it sounds like it might have been specimen vouchered. Is this right? I know the South Africa giant worm typically only 6 feet long. Also are al the Lumbricus terrestris worm in North America european worms that displaced native earthworms? Thanks. bcc to my Zoology class. From romunov from gmail.com Mon Mar 30 20:11:45 2009 From: romunov from gmail.com (romunov) Date: Mon Mar 30 20:16:24 2009 Subject: [Annelida] Biggest earthworm In-Reply-To: <20090330193722.6957571123B@gray.dordt.edu> References: <20090330193722.6957571123B@gray.dordt.edu> Message-ID: <63a206010903301811v456f1a75u6d1d60a3a369014b@mail.gmail.com> FWIW, Invertebrate zoology by Ruppert, Fox and Barnes (2004) lists giant Australian earthworm *Megascolides australis* as the largest oligochaete at around 3 meters. Cheers, Roman On Mon, Mar 30, 2009 at 9:37 PM, James Mahaffy wrote: > Folks, > > I am lecturing on annelids in Zoology and I decided to check the size of > the largest worms. A number of places on the internet give 22 feet as the > size of a record for "The South African Giant Earthworm grows to be up to 22 > feet and is the largest earthworm known. The record-setting specimen was > found beside a road in William's Town, South Africa, in 196&." This came > from http://www.csmonitor.com/2005/0621/p18s02-hfks.html but I saw the > same length quoted on a lot of sites. That almost sounds to be too big to > me but it sounds like it might have been specimen vouchered. > > Is this right? I know the South Africa giant worm typically only 6 feet > long. > > Also are al the Lumbricus terrestris worm in North America european worms > that displaced native earthworms? > > Thanks. > > bcc to my Zoology class. > > > _______________________________________________ > Annelida mailing list > Post: Annelida@net.bio.net > Help/archive: http://www.bio.net/biomail/listinfo/annelida > Resources: http://www.annelida.net > -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/annelida/attachments/20090331/a0617f0e/attachment.html