I am trying to induce calli from root-derived protoplasts and
subsequently to regenerate whole plants from such calli.
I am following the protocol by Mathur J. and Koncz C. published in
the vol. 82 ("Arabidopsis Protocols") of the Methods in Molecular
Biology series (1998).
Unfortunately, I am getting very low efficiency in callus formation
from such protoplasts liquid culture (not embedded).
Can anybody suggest me how to improve this protocol, or what might be
common mistakes I could be doing?
Any comment/advice on regeneration from protoplasts is more than
welcome and appreciated !
Thank you so much for your help!