guodong.wang at wur.nl ("Wang, Guodong") wrote in=20
news:<D3D92867CDE2A64F99E900ABB01E2B2A01AB538E at scomp0017>...
> Dear all,
>> Does anyone have the experience to detect the SM insertion lines within
> PCR methods? I just wonder the primers you use because I cannot get
> anything when I using:
> SM_T-DNA_L4: CGT GAA GTT TCT CAT CTA AGC C. Someone have any idea?
>> All the best,
I was also confused by some other primer recommendations I found in
the web (I think at somewhere at ncbi)
but actually the following primers should do the job:
dSpm 3' end
dspm1: CTT ATT TCA GTA AGA GTG TGG GGT TTT GG (Tm:67.9=B0C)
nested dspm8: GTT TTG GCC GAC ACT CCT TAC C(Tm: 68=B0C)
dSpm 5' end
dspm11: GGT GCA GCA AAA CCC ACA CTT TTA CTT C(Tm: 69=B0C)
nested dspm5: CGG GAT CCG ACA CTC TTT AAT TAA CTG ACA CTC (Tm: 68.0=B0C)
the SM lines are obviously a subgroup of the SLAT lines.
hope that helps.