IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

[Arabidopsis] Gramene Release V.19

Claire E. Hebbard cer17 at cornell.edu
Thu Nov 3 16:19:41 EST 2005

Dear Cereal Researchers,

The Gramene database (<http://www.gramene.org>http://www.gramene.org) 
emails its registered users an announcement each time a new release is 
made. This announcement includes information on new or updated data and 

The Gramene Database

This work is funded by the National Science Foundation (NSF) and the 
USDA-Agricultural Research Service, and was previously funded by the USDA 
Initiative for Future Agriculture and Food Systems (IFAFS). We are thankful 
to numerous collaborators and contributors for help in curation and for 
sharing their datasets and tools.


Gramene website features:

Navigation Bar changes:
"Genome Browser" link on the navigation bar replaced with "Genomes".
"CMap" link on the navigation bar replaced with "Maps".
"Protein" link on the navigation bar replaced with "Proteins".
"Ontology" link on the navigation bar replaced with "Ontologies".
"Gene" link on the navigation bar replaced with "Genes".

New and updated tutorials
All tutorials have been updated. Tutorials are available in HTML, PDF or 
PowerPoint versions to allow for different browser preferences. Many slides 
have notes pages to improve explanations.
Updated tutorials include Maps, Proteins, Ontologies, Genes, QTL and 
Genomes and BLAST tutorials are two individual tutorials, whereas 
previously Genome Browser and Blast were a single tutorial.
New tutorials have been developed for Markers, GrameneMart (a tool in the 
Genomes database), website navigation and an overview of the modules.
Two new exercises have been created to help users conduct an ontology 
search and a comparison between species.


Mapping to the Oryza sativa (japonica) genome of Rice SAGE tags obtained 
from MGOS (Magnaporthe Grisea Oryza Sativa Database).

Mapping to the Oryza sativa (japonica) genome of BAC ends of the following 
species, sequences provided by OMAP project.
Oryza coarctata
Oryza minuta

Mapping to the Oryza sativa (japonica) genome of Maize whole genome shot 
gun reads from JGI (Joint Genome Institute).

Mapping to the Oryza sativa (japonica) genome of Maize genes/loci sequences 
being assayed by the Panzea project, most sequences are maize unigenes.

Updated mapping of OMAP BAC ends for the following species.
Oryza alta
Oryza brachyantha

Updated mapping of PUTs (putative unique transcripts) downloaded from 
Oryza sativa
Hordeum vulgare
Sorghum bicolor
Triticum aestivum
Zea mays

Updated mapping of 3' Sorghum EST cluster from LGB(Laboratory of Genomics 
and Bioinformatics) at University of Georgia. 3' cluster sorghum Milestone 
version 3.0 replaced the 3' cluster sorghum Milestone version 2.0

Updated mapping of Ryegrass assembly by ViaLactia, the genomic sequences 
used by the assembly were cloned from a GeneThresher methyl-filtered library.

1307 of the previously unmapped Rice MPSS (Massively Parallel Signature 
Sequencing) oligo sequences were mapped to the Oryza sativa (japonica) genome.

33 of the previously unmapped Rice Oligos were mapped, these oligos are 
from NSF Rice Oligonucleotide Array Project

The alignment feature query coordinates changed in this release
The alignment block coordinates no longer follow the BLAT output format, 
where the query coordinates are from the point of view of the reverse 
strand when the match is on the reverse strand. The query coordinates are 
always on the forward strand.
All the coordinates are 1-based.
Some track names changed, new names are more accurate, intuitive and 
A new track named "Rice_FSTtransposon" replaced 2 old tracks "Rice_FST_Ds" 
and "Rice_FST_IS"
Oryza Sativa TE (transponsable element) track added at the bottom of the 
detail view.

There is now a BLAST tutorial
    * In this build (v. 19) we present our initial release of the IRGSP 
build 4.0 rice genome assembly, to which we have mapped the RFLP markers 
from rice.
    * We have also added three maps of the recently-released set of Class I 
    * New BAC end sequence from wild Oryza spp. has been added to the map 
of the TIGR rice assembly; some other features have also been updated on 
that map.
    * Nine rice maps been updated with additional QTL and genes.
    * One new QTL map for maize was also added.

New Sequence and Physical Maps New QTL Map Updated Maps
Rice-Gramene IRGSP Assm 2005
Rice-Cornell Class I SSR (TIGR) 2005
Rice-Cornell Class I SSR (IRGSP) 2005
Rice-Cornell Class I SSR (93-11) 2005
Maize Bins QTL 2005

Features Added and Updated
Rice-GR TIGR Assm IRGSP Seq 2005

QTL Added
Rice-IRRI Lemont/Teqing RI RFLP QTL 2001
Rice-JRGP Nipponbare/Kasalath RFLP QTL 2000
Rice-JKU Asominori/IR24 RI RFLP QTL 1996

Genes Added
Rice-Cornell IR64/Azucena SSR 2001
Rice-Cornell BS125/2/BS125/WLO2 RFLP 2001
Rice-IGCN ZhaiYeQing 8/JingXi 17 1998
Rice-IRMI Integrated SSR 2003
Rice-JRGP Nipponbare/Kasalath RFLP 2000
Rice-Hokkaido Morphological 2000

The marker database now contains a total of 4,547,608 markers from more 
than 180 species.
The database has been updated with an additional 13,519 Class I SSRs from 
We have added
2,530,661 Genbank dbEST sequences from fifty species of Poaceae as  marker 
type "EST"
129,527 NCBI Unigene entries from six Poaceae species and 472,408 TIGR Gene 
Indexes from eight Poaceae species as marker type; "EST Cluster"; and
91,074 GenBank DNA sequences from Poaceae flagged as mRNA (excluding dbEST) 
entries from 162 species as marker type "mRNA"

The results table once again shows any number of results, even if more than 
1,000 markers result from a query. The search results are returned 
unsorted, however. Users may still sort the results by clicking on column 
headings, but sorting a large number (> 1,000) of markers will be slow.

Marker breakdown by species:
Species Markers
Barley (Hordeum spp.)                   535720
Maize (Zea spp.)                        1089433
Oat (Avena spp.)                        8215
Rice (Oryza spp.)                       1475049
Rye (Secale spp.)                       13260
Sorghum (Sorghum spp.)          260634
Sugarcane (Saccharum spp.)              339657
Wheat (Triticum spp. + Aegilops spp.)   787493
Other                                   38147


The Gramene protein database now provides curated information on SP-Trembl 
entries from family Poaceae (Grasses)

The protein database includes new and updated information on 68,123 gene 
products drawn from SwissProt (2074) and SP-TREMBL (66,049).

These proteins are annotated using Pfam, Prosite, TMHMM (for transmembrane 
domains), TargetP and Predotar (plastid, mitochrondrial and secretory 
pathway targeting) and Interpro categories. A total of 49,565 gene products 
are associated with 1,479 Gene Ontology terms.

You can search the protein database for entries by using either the 
SWISSPROT accession number / SPTrEMBL ID / Protein Sequence Identifier 
(Protein_ID) / GI number / protein name / gene name / species / cultivar.

The entries for Aegilops, Triticum, Avena and Hordeum link back to 
GrainGenes database.  Similarly the proteins from maize link back to the 

New: The ontology detail page now displays 'External references' and 
'Comments' associated with a given ontology term.

Gramene Taxonomy Ontology: NEW
Total number of terms = 2204. A total of 68,122 proteins, 1488 genes, 9871 
QTL and 163 mapset associations.

Gene Ontology: Updated
Total number of terms = 19,498. A total of 49,565 proteins and 21,369 
Ensembl genes are associated with 1,479 Gene Ontology terms.

Plant Ontology: Updated
Total number of terms = 684. Includes associations to rice genes only

Gramene Plant Growth Stage Ontology: No updates
Total number of terms = 236
The wheat, barley and oat growth stages have the same growth stage ontology.

Trait Ontology: Updated
Total number of terms = 693. A total of 428 genes and 9871 QTL are 
associated with the trait terms.

Environment Ontology:Updated:
Total number of terms = 490

The Ontology browser now displays the total number of objects 
(QTL/phenotype gene/EnsEMBL gene/proteins/mapsets) associated with the 
"term name". For example: genus "Oryza" has following associations:
64,137 data entries
55,238 proteins from various Oryza species
1488 genes
7302 QTL
109 map sets
Genes and Alleles

Continually growing, Gramene release 19 presents a reorganized gene detail 
display page over the last release. Specifically, changes will be noted for 
allele, germplasm, nucleotide and protein sequences, and the associated 
features sections. Associated features included all trait (Trait Ontology), 
growth stage (Plant Ontology), plant anatomy parts (Plant Ontology), and 
gene ontology terms associated with a particular gene. Links to the Gramene 
Ontology have been provided as well.

For release 19, there were 151 genes curated and mapped on several rice 
genetic maps to display as interpolated phenotypes. These map positions 
were based on closely linked markers. All genes with gene symbols beginning 
with the letters "A" through "N" and for which information was available 
are included.

The Gramene Genes Database contains 1,488 genes from rice which have been 
characterized by phenotype. Among them, 425 genes have been fully annotated 
with phenotypic descriptions, associations to trait, plant and gene 
ontologies, map positions, alleles, phenotypic studies, germplasms, 
sequences, gene products, and related public references.


There are 246 newly curated rice QTLs from recent publications that have 
been added to the database.
Collaborative and integrative efforts between Gramene and MaizeGDB 
continue, and 1,215 newly imported maize QTLs from MaizeGDB have been 
integrated into the Gramene QTL database.

Trait Ontology (TO) terms have been integrated with definitions of the 
associated traits of QTL.
This will also allow the users to browse QTL by searching traits in the 
Gramene Ontology Database.

The Gramene QTL database includes a total of 9,871 QTL
These QTL were identified for numerous agronomic traits in rice, maize, 
barley, oat, wheat, pearl millet, foxtail millet and wild rice.

Subscribe to the Gramene-announce list to receive announcements of updates
to the Gramene site. To subscribe to a mailing list, send an e-mail to
majordomo at gramene.org. In the body of the e-mail write:
subscribe gramene
subscribe gramene-announce
Claire Hebbard
Gramene Outreach Coordinator
G15 Bradfield Hall
Ithaca, NY 14853

Cereals are mankind's most important source of calories. Gramene can assist 
in research on agriculturally important features in rice, maize, wheat and 
other grasses. 
-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://www.bio.net/bionet/mm/arab-gen/attachments/20051103/4bc44d47/attachment-0001.html

More information about the Arab-gen mailing list

Send comments to us at biosci-help [At] net.bio.net