I am writing regarding your question about Agrobacterium mediated transformation. As I have experienced after 48 hours of co-cultivation, the gene expression starts. As soon as the gene enters in the nucleus its transcription starts. Moreover its further expression depends upon the integration position of gene in the chromosome. I have observed that in gene gun transformation, next day expression was observed.
This is my understanding on the topic.
Muhammad Shahzad Anjam
Lecturer Biotechnology, Institute of Biotechonology ,
Bahauddin Zakariya University , Multan (www.bzu.edu.pk)
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