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[Arabidopsis] Re: Arab-gen Digest, Vol 55, Issue 4

Hendrik Poorter via arab-gen%40net.bio.net (by h.poorter from gmail.com)
Fri Nov 6 12:29:55 EST 2009

In reply to the question of Eric Brenner about teaching and Arabidopsis in
intro biology:

At Utrecht University we have a one week course (divided over 2 weeks) on
the experimental cycle. Students have to follow this cycle almost full
circle. We pregrow a number of Arabidopsis mutants (sex-1 of course, always
a favourite, pgm, but also a few hormone mutants) and their WT for them in
the growth chamber, in pots, at 2 light intensities. The measurements they
have to do are necessarily fixed already beforehand, but they have to find
information about their mutant on the web and in the literature, and then
they have to formulate the exact question and the hypothesis they have, both
about the effect of light and of the mutation.

Then they measure traits at 3 levels: gene expression of Rubisco and a CAB
gene by qPCR, Rubisco and chlorophyll content, and specific leaf area, along
with dry weight:fresh weight ratio's, leaf size and shape, leaf angle and
what they like to measure more.

They have to calculate a number of variables and enter them all on papers
with predesigned tables with the relevant traits. In this way we can see
whether relative differences in gene expression translate into a differences
in the Chlorophyll:Rubisco ratio, and how these changes relate to
differences in SLA, or concentration per unit fresh or dry weight. They have
to draw their conclusions, make a poster and present these for other
students of their year.

Great fun! Success,

Hendrik Poorter

On Fri, Nov 6, 2009 at 6:03 PM, <arab-gen-request from oat.bio.indiana.edu>wrote:

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> Date: Thu, 05 Nov 2009 10:09:55 -0500
> From: Eric D Brenner <eb50 from nyu.edu>
> Subject: [Arabidopsis] Arabidopsis in teaching intro. biology
> To: arab-gen from magpie.bio.indiana.edu
> Message-ID: <5f00e3b437e5de.4af2a473 from mail.nyu.edu>
> Content-Type: text/plain; charset=us-ascii
> Dear Colleagues,
>  I would like to know if any of you use Arabidopsis in introductory biology
> teaching courses?  If so, would you be so kind as to share with me the
> experiment that you perform, the strains/mutants used, and a general idea in
> how long the experiment takes, and the source and cost of materials?
> Thank you ever so kindly,
> Eric D. Brenner, PhD.
> Clinical Assistant Professor
> Department of Biology
> New York University
> 609 Silver Building
> 100 Washington Square East
> New York, NY 10003
> wk: 212-992-8693
> fx: 212-995-4204
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> End of Arab-gen Digest, Vol 55, Issue 4
> ***************************************

Dr. Hendrik Poorter
ICG-3; Research Institute Jülich
D-52428 Jülich; Germany

tel: +49-(0)2461-61-6833

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