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[Arabidopsis] Latest methods to to check superoxide, hydrogen peroxide and mitochondrial membrane potential in plants

Rosemary.White from csiro.au via arab-gen%40net.bio.net (by Rosemary.White from csiro.au)
Sun Jan 5 16:42:17 EST 2014

Dear Prakash,

The probes peroxy orange and peroxy yellow are pretty good for hydrogen
peroxide and certainly work in Arabidopsis. There are others out there as
well - see http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2862989/
or http://pubs.acs.org/doi/abs/10.1021/ja1014103
and citing articles.


Dr Rosemary White
CSIRO Plant Industry
GPO Box 1600
Canberra, ACT 2601

T 61 2 6246 5475
F 61 2 6246 5334
E rosemary.white from csiro.au

On 3/01/14 4:00 PM, "Prakash M G" <pmgnair from gmail.com> wrote:

>Dear All,
>Greetings !
>Please provide details about the most modern methods (modern fluorescent
>probes that are available commercially) to determine superoxide, hydrogen
>peroxide and mitochondrial membrane potential in plants.
>I used DCFH-DA, DAB, NTB and Rh123 but seems that all these techniques
>not accepted by journals ?
>Thank you all
>with kind regards
>Arab-gen mailing list
>Arab-gen from net.bio.net

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