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[Arabidopsis] NCSU Recombineering Center

Jose Alonso via arab-gen%40net.bio.net (by jmalonso from ncsu.edu)
Wed Jan 15 11:12:59 EST 2014


Dear Arabidopsologists,



North Carolina State University has just opened a cost-recovery service to
generate whole-gene translational fusions by means of recombineering (for
more details, see Zhou et al. 2011) in the context of transformable
bacterial artificial chromosomes. The constructs produced are suitable for
stable *Agrobacterium*-mediated transformation of Arabidopsis.

The recombineering approach has many advantages over the conventional
cloning strategies, among them the ability: 1) to tag any gene at any
position independently of the size of the gene, and 2) to ensure that the
expression pattern obtained faithfully mimics that of the endogenous gene.
This can be achieved by including in the final construct large regions (at
least 10kb, but customizable to almost any other size) of the genomic
sequences flanking the gene of interest. Different fluorescent tags such as
GFP, Ypet, or mCherry can be used. Additional tags could be generated. If
you are interested in utilizing this service or have any questions, please,
contact us at jmalonso from ncsu.edu or visit our webpage at
http://www4.ncsu.edu/~atstepan/Welcome.html



With best regards,



Jose M. Alonso

-- 
Jose Alonso
Phone: (919)515 5729
Fax: (919) 515 3355
Email: jmalonso from unity.ncsu.edu
Web: http://www4.ncsu.edu/~jmalonso


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