Hi Happy Sequencers
(1) I have been using Page Plus for sometime now and using
P.E./ABI protocol with 36cm plates on the 377 I achive good
lengths 700 + (1-2% N) - when given good DNA !
So has anyone optimised a protocol for the 48cm plates to get
even longer reads- the magic figure of 1000bp beckons.
(2) A second question which I am sure someone knows the answer
to what is that tasteful pink dye in the Amersham loading
buffer. Such a different attitude - Amersham include a loading
buffer and Am acetate in their new kits which are also cheaper.
(3) Spin-Columns - I use the Pharmacia Microspin G-50 , I know of
the Centri-Sep (expensive), are there any other makes which
people out there use ?