Dear newsgroup,
I have some problems according to my RNA-RNA in-situ hybridisation.
I get no specific labeling with my antisense probe, produced by the DIG-RNA
labeling Kit from Roche. The hybridization temperature is 50 °C. We tried
the probes at paraffin-embedded tissues treated with proteinase K and also
at tissues that were cut with a cryostat.
I tried several protocolls but it would be nice if someone could send me his
or her protocolls.
I am not sure if our sense and antisense probes work properly. To test them
I have put them on a formaldehyd-gel. One probe shows one distinct band. The
other one also has a band at the same size but there is smear around it.
Have you any experience which "gel-picture" of the probes is better?
Thanks a lot in advance.
Regards
Frank
