On Sun, 2 Mar 2008 05:09:08 -0800 (PST), merhawi debesai
<samiboy_4ever from yahoo.com> wrote:
>Hi,
> Thank you so much for your information in advance.
> Currently, I am working on the physico-chemical characterization of EPS from three strains of biofilm bacteria Pseudomonas aeruginosa, using QCM-D. Thus, I have questions:
>
> 2. The second question is regarding the preparation of bacterial strains for
> surface analysis.
> i. Usually, we prepare the test strains in suspensions. However, it is
> known that there....difference between planktonic and sessile (biofilm)
> bacteria. Thus,AREN'T WE TAKING THE WRONG STRAIN?!
I think you just answered your own question.
Not sure if they make a different EPS when sessile, rather than just
more of it. But seems to me you would want to at least check.
Once you get your methodology running, try comparing.
By the way, there is a nice overview article in February issue of
Microbe:
How Pseudomonas aeruginosa Regulates Surface Behaviors
George A. O’Toole
At surfaces, these bacteria either form biofilms or swarm, a
regulated behavior with
important consequences for pathogenesis
http://www.asm.org/microbe/index.asp?bid=55982
bob
