From sales from kanecki.com Thu May 1 12:28:47 2008 From: sales from kanecki.com (dk) Date: Thu May 1 15:42:05 2008 Subject: [Cell-biology] Announcement - New Program for Flow Cytometry Analysis Message-ID: <5d615d23-05c6-4986-b667-ec1faf23bc8d@s50g2000hsb.googlegroups.com> Hi, My company has developed a new flow cytometry program that works with BD, Coulter/Ortho, and Partec sample files. A free version is available at: http://sourceforge.net/projects/kflowcytometryq and is distributed under the GNU Public License 3.0. We believe in open standards. Sincerely, David Kanecki, MBA, ACS, Bio. Sci. President Kanecki Associates, Inc. www.kanecki.com P.S: My background in medical research includes working as a researcher at the Milwaukee Medical Research park from 1989-1993. Also, I am certified in flow cytometry, cellular biology, and immunology by BD and the National Flow Cytometry Resource. At the Milwaukee Medical Research Park, I managed a $10 M laboratory and was part of the team that established a program project grant. Currently, I work on medical research and medical software at my own company, Kanecki Associates, Inc. From DENNIST from ascb.org Fri May 9 09:24:17 2008 From: DENNIST from ascb.org (David Ennist) Date: Fri May 9 10:49:01 2008 Subject: [Cell-biology] invitation to submit images to ASCB Image & Video Library Message-ID: All, I would like to invite you to submit cell biology images and videos to the Image & Video Library of the American Society for Cell Biology. I know that people such as yourself come across a number of stunning images that don't get published - this is an attempt to share these images with a wider audience. We also welcome submissions from students. Have a look at our holdings: http://cellimages.ascb.org and follow the link under "Submit Resources" to set up an account and make your submissions. They will be reviewed by a member of our Editorial Board (Kerry Bloom, Univ North Carolina, Chapel Hill is our Editor-in Chief). We're currently developing a website called CellBASE with resources targeted at more defined topics, including a set of microscopy links - we're still building the content, but you can have a look at http://cellbase.ascb.org . I'm looking forward to your submissions! Please feel free to email or call if you have any questions. Cheers, Dave David L. Ennist, PhD Director, Digital Resources The American Society for Cell Biology 8120 Woodmont Avenue, Suite 750 Bethesda, MD 20814-2762 TEL: 301-347-9315 FAX: 301-347-9310 email: DEnnist@ascb.org website: http://cellimages.ascb.org/ From catherine.omahony from ucc.ie Wed May 21 05:26:01 2008 From: catherine.omahony from ucc.ie (O'Mahony, Catherine) Date: Wed May 21 21:24:31 2008 Subject: [Cell-biology] New conference event for calendar Message-ID: <7768701FBD6B4D4F9FD0141957CAB6E60444C574@EXCH1.central.ad.ucc.ie> The Agricultural Biotechnology International Conference is returning to Europe this August and will be hosted by University College Cork in Cork, Ireland. The annual ABIC conference brings many of the world's largest agriculture, fisheries and food biotechnology companies to the host country (many Fortune 500 companies) and this year is no exception. At ABIC 2008 in Cork, there will be many hot topics under discussion and debate including innovations in areas such as biofuels and bioenergy, animal and plant breeding, fisheries biotech, molecular pharming, dairy and food, including dedicated sessions on policy, regulatory affairs and business strategies. Registration for the conference has now opened and full details of ABIC 2008 are available on the conference website. http://www.abic.ca/abic2008/html/program.html From sittner from lkb.ens.fr Wed May 21 16:27:26 2008 From: sittner from lkb.ens.fr (assa sittner) Date: Wed May 21 21:33:17 2008 Subject: [Cell-biology] permeabilize living cells Message-ID: Hi there, I am looking for a reagent that would allow me to weakly permeabilize, or just weaken cell membranes in a living cell culture, without killing them, in order to facilitate microinjection. does anyone know such a reagent (most likely a mild surfactant)? cheers, t From nick.theodorakis from gmail.com Fri May 23 08:50:53 2008 From: nick.theodorakis from gmail.com (Nick Theodorakis) Date: Fri May 23 11:48:40 2008 Subject: [Cell-biology] Re: permeabilize living cells References: Message-ID: On May 21, 5:27?pm, "assa sittner" wrote: > Hi there, > I am looking for a reagent that would allow me to weakly permeabilize, or > just weaken cell membranes in a living cell culture, without killing them, > in order to facilitate microinjection. > does anyone know such a reagent (most likely a mild surfactant)? > cheers, > t I've seen a couple of references where people have used low concentrations of mild detergents, but cells usually hate it when you make then leaky. If you are injecting regular cultured cells with a glass microcapillary there should be no reason to make the membrane weaker; the capillary will go right through. Nick -- Nick Theodorakis nick_theodorakis@hotmail.com contact form: http://theodorakis.net/contact.html From joeosborne Tue May 27 17:47:00 2008 From: joeosborne (joeosborne) Date: Tue May 27 12:46:47 2008 Subject: [Cell-biology] Re: permeabilize living cells References: Message-ID: <483ba792$0$21154$5a62ac22@per-qv1-newsreader-01.iinet.net.au> i have read research that pulsed electric fileds at certain frequencies transiently cause cancer cells to become porous thus enabling increased delivery of chemotherapeutic agents into the cell the pulse by itself as used on living tissue isn't designed to kill the cells it just induces them to open channels through which substrates will flow search "electroporation cell membrane" at http://www.ncbi.nlm.nih.gov/sites/entrez on the internet there is a video of a news clip of the principle being used to treat a man for melanoma on his foot it's not a chemical reagent but it does make them permeable as far as chemicals go you are on the right track with surfactants - i also have come across "saponins" which will do the same thing search "saponin permeable cell membrane" at http://www.ncbi.nlm.nih.gov/sites/entrez there are some books that talk in detail about plant saponins in this regard on google books i have read plant saponins are used to make nanoparticles for drug delivery of chemo drugs too perhaps they facilitate penetration of the drug conjugate through the cell membrane ----- Original Message ----- From: "assa sittner" Newsgroups: bionet.cellbiol To: Sent: Wednesday, May 21, 2008 2:27 PM Subject: permeabilize living cells > Hi there, > I am looking for a reagent that would allow me to weakly permeabilize, or > just weaken cell membranes in a living cell culture, without killing them, > in order to facilitate microinjection. > does anyone know such a reagent (most likely a mild surfactant)? > cheers, > t From dr_hcchauhan from yahoo.co.in Thu May 29 07:26:53 2008 From: dr_hcchauhan from yahoo.co.in (harsad chauhan) Date: Thu May 29 16:21:45 2008 Subject: [Cell-biology] regarding PBS Message-ID: <10643.73230.qm@web94014.mail.in2.yahoo.com> Respected Sir, Pl. let me know about PBS and Phosphate saline buffer. Whether they are same or different/ What is the composition Thanking you Yours faithfully, h c chauhan --------------------------------- Unlimited freedom, unlimited storage. Get it now From R.Jayakumar from roswellpark.org Thu May 29 16:56:55 2008 From: R.Jayakumar from roswellpark.org (Jayakumar, R) Date: Fri May 30 13:25:52 2008 Subject: [Cell-biology] regarding PBS In-Reply-To: <10643.73230.qm@web94014.mail.in2.yahoo.com> References: <10643.73230.qm@web94014.mail.in2.yahoo.com> Message-ID: <97101976F8A044468CA74FE11883B90E173E7A75@VISTA.roswellpark.org> PBS= phosphate buffered saline. You can get it from the internet Jai -----Original Message----- From: cellbiol-bounces@oat.bio.indiana.edu [mailto:cellbiol-bounces@oat.bio.indiana.edu] On Behalf Of harsad chauhan Sent: Thursday, May 29, 2008 8:27 AM To: cellbiol@magpie.bio.indiana.edu Subject: [Cell-biology] regarding PBS Respected Sir, Pl. let me know about PBS and Phosphate saline buffer. Whether they are same or different/ What is the composition Thanking you Yours faithfully, h c chauhan --------------------------------- Unlimited freedom, unlimited storage. Get it now _______________________________________________ Cellbiol mailing list Cellbiol@net.bio.net http://www.bio.net/biomail/listinfo/cellbiol This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you.