From kobayash from nature.berkeley.edu Thu May 8 18:46:44 2008 From: kobayash from nature.berkeley.edu (Marilyn Kobayashi) Date: Thu May 8 21:58:54 2008 Subject: [Chlamydomonas] Frameshift mutagens Message-ID: <482390E4.2030004@nature.berkeley.edu> We are developing a reverse genetics strategy for finding mutations in specific genes. Presently we are using uv mutagenesis but would like to try other mutagens to get a broader spectrum of mutants. We have tried a frameshift mutagen, ICR-191. Does anyone have any experience to maximize the mutation rate using ICR-191? Is there other frameshift mutagens we could try with chlamy? Thanks, Marilyn -- <>-<>-<>-<>-<>-<>-<>-<>-<>- Marilyn Kobayashi Niyogi Lab 441 Koshland Hall Plant and Microbial Biology University of California Berkeley, CA 94720 510-643-6604 From pmp5 from courant.nyu.edu Sun May 11 19:07:05 2008 From: pmp5 from courant.nyu.edu (Peter Palenchar) Date: Mon May 12 07:44:11 2008 Subject: [Chlamydomonas] arabidopsis orthologs Message-ID: I'm looking for where somebody has assigned the relationships between an algae and Arabidopsis at the genome level. Something like what the Homologene database is doing, but they don't seem to include any algae. Peter Palenchar From dutcher from genetics.wustl.edu Tue May 20 07:40:00 2008 From: dutcher from genetics.wustl.edu (Susan Dutcher) Date: Tue May 20 08:06:45 2008 Subject: [Chlamydomonas] GreenGenie2 Message-ID: GreenGenie2 is an updated and retrained version of Genie for predicting genes in Chlamydomonas. It is trained on 2600 EST assemblies. The link for submitting your DNA sequence is below. http://bifrost.wustl.edu/cgi-bin/greengenie2/greenGenie2 Susan K. Dutcher Professor and Interim Chair Department of Genetics Department of Cell Biology and Physiology Washington University School of Medicine St. Louis, MO 63110 dutcher@WUSTL.edu 314-362-2765