In article <1993Aug6.155222.293 at chmeds.ac.nz> tdw at chmeds.ac.nz () writes:
>A friend is looking for the X-ray crystalography structure of fibrinogen, and
>or fibrinogen parts. Any help much appreciated.
>>tdw at twizon.chmeds.ac.nz>
Hmm, the only thing I could find is a compex with a few residues of
fibrinogen - it's Pre-release from PDB:
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HEADER PRELIMINARY 27-APR-92 P1BBR
COMPND BOVINE THROMBIN (E.C.3.4.21.5) COMPLEX WITH RESIDUES 7 - 16
COMPND 2 OF FIBRINOPEPTIDE-A ALPHA
SOURCE BOVINE (BOS $TAURUS) BLOOD
AUTHOR P.MARTIN,B.EDWARDS
1
1 REFERENCE 1
1 AUTH P.D.MARTIN,W.ROBERTSON,D.TURK,R.HUBER,W.BODE,
1 AUTH 2 B.F.P.EDWARDS
1 TITL THE STRUCTURE OF RESIDUES 7-16 OF THE A ALPHA-CHAIN
1 TITL 2 OF HUMAN FIBRINOGEN BOUND TO BOVINE THROMBIN AT
1 TITL 3 2.3-*ANGSTROMS RESOLUTION
1 REF J.BIOL.CHEM. V. 267 7911 1992
1 REFN ASTM JBCHA3 US ISSN 0021-9258 071
2
2 RESOLUTION. 2.3 ANGSTROMS.
3
3 REFINEMENT.
3 PROGRAM PROLSQ
3 AUTHORS KONNERT AND HENDRICKSON
3 R VALUE 0.167
3 RMSD BOND LENGTHS 0.020 ANGSTROMS
3 RMSD BOND ANGLES 3.3 DEGREES
4
5 THE SEQUENCE IS IN THE THROMBIN NUMBERING CONVENTION WITH
5 RESIDUE 1 AT THE POINT IT IS CLEAVED FROM PROTHROMBIN AND
5 ENDING WITH 308. FIBRINOPEPTIDE SEQUENCE NUMBERS ARE 309
5 TO 318 AND CORRESPOND TO RESIDUES 7 TO 16 OF FIBRINOGEN-A
5 ALPHA. THE N-TERMINUS OF THE FIBRINOPEPTIDE IS ACETYLATED.
5
6 THERE ARE THREE INDEPENDENT COMPLEXES IN THE ASYMMETRIC
6 UNIT. MOLECULE I IS EPSILON-THROMBIN (CHAIN BREAK BETWEEN
6 RESIDUES 199 AND 200), AND MOLECULES II AND III ARE
6 ALPHA-THROMBIN. FIBRINOPEPTIDE I HAS AN OCCUPANCY THAT
6 MAKES THE AVERAGE B OF ARG-318 ROUGHLY EQUAL TO THAT IN
6 FIBRINOPEPTIDES II AND III. THE DEPOSITORS DO NOT SEE ANY
6 DENSITY AT ALL FOR THROMBIN RESIDUES 1-13, AND POOR DENSITY
6 FOR THE N AND C TERMINI OF THE A CHAINS, THE C TERMINUS OF
6 THE B CHAINS, AND THE FIVE RESIDUES BORDERING 200 IN
6 MOLECULES II AND III.
FTNOTE 1
FTNOTE 1 RESIDUES PRO T 72, PRO U 72, AND PRO V 72 ARE CIS PROLINES.
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If this will help you can retrieve the entire structure with gopher -
write me a note if you've never heard of gopher, I'll send you some info to
help get you started.
Best of luck,
Dan Jacobson
danj at welchgate.welch.jhu.edu
