In article <1993Jun16.134141.47 at cnbvx3.cnb.uam.es> mrege at cnbvx3.cnb.uam.es writes:
>Hi, Netters,
>>This is to report an error in the EMBL nucleic acid database.
>We are looking for the gene and protein sequence of lom, a gene from
>bacteriophage lambda: Barondess and Beckwith (1990) 'A bacterial virulence
>determinant encoded by lysogenic coliphage lambda' Nature, 346:871-74.
>>The sequence we retrieved from the database was BLAMLOM X55792, which
>has found to be the sequence of the gene 'bor', described in the same
>paper. Searching for 'bor' did not retrieve any sequence, nor lom nor bor.
>>Please, can anyone find for us this sequence, or is it definitively lost
>in the cyberspace?
>
A quick gopher search of EMBL for:
lom or bor
finds the following two entries.
-----------
ID BLAMLOM standard; DNA; PHG; 326 BP.
XX
AC X55792;
XX
DT 01-APR-1993 (Rel. 35, Created)
DT 01-APR-1993 (Rel. 35, Last updated, Version 1)
XX
DE Bacteriophage lambda lom gene
XX
KW envelope protein.
XX
OS Bacteriophage lambda
OC Viridae; ds-DNA nonenveloped viruses; Siphoviridae.
XX
RN [1]
RP 1-326
RA Barondess J.J., Beckwith J.;
RT "A bacterial virulence determinant encoded by lysogenic coliphage
RT lambda";
RL Nature 346:871-874(1990).
XX
FH Key Location/Qualifiers
FH
FT source 1..326
FT /organism="Bacteriophage lambda"
FT CDS 24..317
FT /gene="lom"
XX
SQ Sequence 326 BP; 104 A; 72 C; 69 G; 81 T; 0 other;
caaaaagcat cgggaataac accatgaaaa aaatgctact cgctactgcg ctggccctgc 60
ttattacagg atgtgctcaa cagacgttta ctgttcaaaa caaaccggca gcagtagcac 120
caaaggaaac catcacccat catttcttcg tttctggaat tgggcagaag aaaactgtcg 180
atgcagccaa aatttgtggc ggcgcagaaa atgttgttaa aacagaaacc cagcaaacat 240
tcgtaaatgg attgctcggt tttattactt taggcattta tactccgctg gaagcgcgtg 300
tgtattgctc acaataattg catgag 326
//
--------------------------
ID BLAMBOR standard; DNA; PHG; 701 BP.
XX
AC X55793;
XX
DT 01-APR-1993 (Rel. 35, Created)
DT 01-APR-1993 (Rel. 35, Last updated, Version 1)
XX
DE Bacteriophage lambda bor gene
XX
KW envelope protein.
XX
OS Bacteriophage lambda
OC Viridae; ds-DNA nonenveloped viruses; Siphoviridae.
XX
RN [1]
RP 1-701
RA Barondess J.J., Beckwith J.;
RT "A bacterial virulence determinant encoded by lysogenic coliphage
RT lambda";
RL Nature 346:871-874(1990).
XX
FH Key Location/Qualifiers
FH
FT source 1..701
FT /organism="Bacteriophage lambda"
FT CDS 66..686
FT /gene="bor"
XX
SQ Sequence 701 BP; 157 A; 144 C; 232 G; 168 T; 0 other;
ctgagtgtgt tacagaggtt cgtccgggaa cgggcgtttt attataaaac agtgagaggt 60
gaacgatgcg taatgtgtgt attgccgttg ctgtctttgc cgcacttgcg gtgacagtca 120
ctccggcccg tgcggaaggt ggacatggta cgtttacggt gggctatttt caagtgaaac 180
cgggtacatt gccgtcgttg tcgggcgggg ataccggtgt gagtcatctg aaagggatta 240
acgtgaagta ccgttatgag ctgacggaca gtgtgggggt gatggcttcc ctggggttcg 300
ccgcgtcgaa aaagagcagc acagtgatga ccggggagga tacgtttcac tatgagagcc 360
tgcgtggacg ttatgtgagc gtgatggccg gaccggtttt acaaatcagt aagcaggtca 420
gtgcgtacgc catggccgga gtggctcaca gtcggtggtc cggcagtaca atggattacc 480
gtaagacgga aatcactccc gggtatatga aagagacgac cactgccagg gacgaaagtg 540
caatgcggca tacctcagtg gcgtggagtg caggtataca gattaatccg gcagcgtccg 600
tcgttgttga tattgcttat gaaggctccg gcagtggcga ctggcgtact gacggattca 660
tcgttggggt cggttataaa ttctgattag ccaggtaaca c 701
//
Best of luck,
Dan Jacobson
danj at welchgatee.welch.jhu.edu
