Hello,
In article <3hqnis$788 at ixnews3.ix.netcom.com>, krokos at ix.netcom.com
(Christopher Krokos) writes:
>>Can anyone help me to find a following freeware or shareware DOS/Windows
>programs:
>> Primer Design Program
> Probe Design Program
> PCR Design Program
>>Thank you in advance for any information,
>>Sincerely yours,
>>Christopher A Krokos
>E-mail: krokos at ix.netcom.com>
The BioCatalog at EBI will porvide this information
(URL: http://www.ebi.ac.uk/biocat/biocat.html)
Here is the only entry for a freeware or shareware DOS/Windows program:
AC BC00155
NAME OSP
DOMAIN Oligomer design and synthesis
DESCRIPTION OSP is a computer program developed to aid in
DESCRIPTION selecting oligonucleotide primers for DNA
DESCRIPTION sequencing and for the polymerase chain
DESCRIPTION reaction. OSP allows the user to specify (or
DESCRIPTION use default) constraints for primer and
DESCRIPTION amplified product lengths, %G+C contents,
DESCRIPTION and (absolute or relative) meltingtemperatures
DESCRIPTION for primer 3' nucleotides; and for the maximum
DESCRIPTION allowable primer-self,primer-primer, and
DESCRIPTION primer-product annealing propensities.
DESCRIPTION Candidate primer sequences are screened
DESCRIPTION against a user-supplied data set of other
DESCRIPTION sequences (e.g. repetitive element or vector
DESCRIPTION sequences) to help minimize the possibility of
DESCRIPTION non-specific priming.
DESCRIPTION Primers meeting all constraints are ranked and
DESCRIPTION displayed in order of increasing overall
DESCRIPTION ``score'', which is a user-definable weighted
DESCRIPTION sum of the above parameter values.
DESCRIPTION The program is available at no cost from the
DESCRIPTION authors in two versions, one of which produces
DESCRIPTION text-only output, and the other having an
DESCRIPTION interactive X windows graphic interface.
AUTHOR LaDeana Hillier
RA Hillier L, Green P.;
RT "OSP: A computer program for choosing PCR
RT and DNA sequencing primers.";
RL PCR Methods and Applications 1:124-128(1991).
RX Medline; 93113324.
RX SeqAnalRef; HILL9101.
ADDRESS Washington University Medical School
ADDRESS Department of Genetics, Box 8232, 4566 Scott,
ADDRESS St. Louis, MO 63110 USA.
CONTACT LaDeana Hillier lfw at elegans.wustl.edu
SITE -
SITE-CONTACT -
OS VAX/VMS, PC, MAC, and SUN Sparcstations),
OS or interactive X windows graphics version
OS (X-Window version for the Sparc and now for Linux).
LANGUAGE C
Regards,
Pat
--
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EBI - EMBL outstation | URL: http://www.ebi.ac.uk
Hinxton Hall, Hinxton | Tel: +44 (0)223 494 413
Cambridge CB10 1RQ, UK | Fax: +44 (0)223 494 468
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