FNINER (fniner at aol.com) wrote:
: I am interested in obtaining nucleotide sequences for HUMAN Janus family
: of protein tyrosine kinases (JAK1, JAK2, JAK3, and Tyk2) and also various
: HUMAN Signal Transducers and Activators of Transcription (STAT1 - STAT6)
: proteins. I would greatly appreciate any information concerning the
: accession numbers for these genes. Many thanks in advance.
:FNINER at AOL.COMIf you have access to Mosaic or Netscape, you can easily answer this
question yourself.
The Sequence retrieval system (SRS) servers allow you to select information
from a wide range of sequence and sequence related databases.
There are several SRS Servers, I describe here how to work with the EBI one
Except for the URL, the other will work similarly
Access the following URL:
http://www.ebi.ac.uk/srs/srsc
or http://www.ebi.ac.uk
select [database query/retrieval]
select [Sequence Retrieval System]
select [search sequence libraries]
select the database(s) you want to search, type in the keywords of interest
and select [do-query]
I selected embl+emnew
and the following keywords:
Definition: JAK
Organism: homo
Alltext: tyrosine
which gave me:
ID HS09607 standard; RNA; PRI; 4064 BP.
XX
AC U09607;
XX
DT 14-JUL-1994 (Rel. 40, Created)
DT 14-JUL-1994 (Rel. 40, Last updated, Version 1)
XX
DE Human JAK family protein tyrosine kinase (JAK3) mRNA, complete cds.
...
D HSPTKJAK standard; RNA; PRI; 3541 BP.
XX
AC M64174; M35203;
XX
DT 12-APR-1991 (Rel. 28, Created)
DT 20-JAN-1993 (Rel. 34, Last updated, Version 2)
XX
DE Human protein-tyrosine kinase (JAK1) mRNA, complete cds.
XX
...
ID HS76112 standard; RNA; EST; 321 BP.
XX
AC T29761;
XX
DT 09-JAN-1995 (Rel. 42, Created)
DT 09-JAN-1995 (Rel. 42, Last updated, Version 1)
XX
DE EST93948 Homo sapiens cDNA 5' end similar to tyrosine kinase JAK1
DE (HT:92).
XX
...
Exchanging TYK for JAK, gave
XX
AC X54637;
XX
DT 05-NOV-1990 (Rel. 25, Created)
DT 12-SEP-1993 (Rel. 36, Last updated, Version 2)
XX
DE Human tyk2 mRNA for non-receptor protein tyrosine kinase
XX
KW protein tyrosine kinase.
I leave the query with the STAT's to you, I do not want to take all the
fun out of it :-)
--
Jeroen
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