IUBio

searching for viral genes

shano o970028r at eds.ecip.nagoya-u.ac.jp
Fri Jul 10 10:05:46 EST 1998


I'm trying to find a glycoprotein gene of a baculovirus using as a
probe a clone which contains part of the same glycoprotein sequence but
from another related baculovirus. When I finally got positive Southern
hybri.results and sequenced one of the clones I found that the clone I
got has a transposable element which has carried part of a gene from its
host. Has that ever happen to you or can you explain what's wrong?
thanks for you help, 'cause this is causing a lot of 
problems to me.



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