Dear Discussion Group,
I would like to know how can I use currently existing bacterial 16s rRNA
database to design gruop specific probes (within region of 1000 to 1400 of
16s rRNA sequence) in order to detect soil bacteria such as gram + with
high G+C, gram + with low G+C, Alpha proteobacteria, Beta proteobacteria,
Gamma proteobacteria,Delata proteobacteria,Cytophaga-Flavobacterium and
General eubacteria. Could anyone of this discussion group give me some
valuable comment and suggestion. Thanks for your attention and time on this
matter. Your help will be highly appreciated.
Best regards,
Samuel Lam
Research Assistant
Department of Biology and Chemistry
City University of Hong Kong
HONG KONG
Tel: (852) 2784 4956; Fax: (852) 2784 4447
email: bhslam at cityu.edu.hk