IUBio

design bacterial probes

Eduard Post E.post at biol.rug.nl
Mon May 31 09:41:21 EST 1999


I think the best way to develop probe's or primers is with the use of ARB, this
program runs on linux.

"Samuel K.S. LAM" wrote:

> Dear Discussion Group,
>
> I would like to know how can I use currently existing bacterial 16s rRNA
> database to design gruop specific probes (within region of 1000 to 1400 of
> 16s rRNA sequence) in order to detect soil bacteria such as gram + with
> high G+C, gram + with low G+C, Alpha proteobacteria, Beta proteobacteria,
> Gamma proteobacteria,Delata proteobacteria,Cytophaga-Flavobacterium and
> General eubacteria. Could anyone of this discussion group give me some
> valuable comment and suggestion. Thanks for your attention and time on this
> matter. Your help will be highly appreciated.
>
> Best regards,
> Samuel Lam
> Research Assistant
> Department of Biology and Chemistry
> City University of Hong Kong
> HONG KONG
> Tel: (852) 2784 4956; Fax: (852) 2784 4447
> email: bhslam at cityu.edu.hk




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