I've seen one such submission - I think it was an arenavirus sequence,
and the reason I found it was because I was searching for matches to a
truly palindromic sequence - in the original grammatical sense - like
"OTTO". One hit, also annotated as arenavirus, made no sense except
for the searched motif - until I noticed that the entire sequence was
submitted backwards. Can this phenomenon have been caused by
misorientation while reading sequence autoradiographs - like reading
the film from the wrong side?
Olav
On Sun, 28 Nov 1999 17:58:43 +0100, "Dr. A.H.C. van Kampen"
<kwekkwek at xs4all.nl> wrote:
>Hello,
>>The nucleotide sequences in the EMBL should all be in either the sense
>5'-3'(+) orientation or in the anti-sense orientation 3'-5'(-). Does
>anyone
>has any idea how many sequences are submitted to EMBL in the wrong
>orientation, i.e. either only reverse 3'-'5'(+) or complement 5'-3'(-)?
>>Regards,
>>--
> --------------------------------------------------------------
> | Dr. Antoine H.C. van Kampen
> | Bioinformatics Laboratory
> | D01-404, ADB/ICT-services
> | Academic Medical Center Amsterdam
> | PO Box 22660, 1100 DD Amsterdam, the Netherlands
> --------------------------------------------------------------
> | Phone: ++31-20-5667096
> | Fax: ++31-20-5669020
> | Email: a.h.vankampen at amc.uva.nl> | www: http://www.amc.uva.nl/> -------------------------------------------------
>>
--
________________________________________________________________________
Olav Hungnes olav.hungnes at labmed.uio.no
Natl.Inst.of Public Health, Dept.of Virology olav.hungnes at embnet.uio.no
P.O.Box 4404 Torshov, phone: (+47)22 04 25 20
N-0403 OSLO, NORWAY FAX: (+47)22 04 24 47
