SYG motif in other proteins

William J. Buikema wjb1 at ellis.uchicago.edu
Thu Jun 1 10:09:09 EST 1995

In article <3qkcda$4p6 at netnews.upenn.edu>,
Edward Zartler <zartler at a.chem.upenn.edu> wrote:
>	Helloooooooooo.
>	My question is simple, we came upon this protein in looking at other 
>stuff, and we had an idea can any body help me.
>	The chromphore is formed by the SYG at 64-66 (?).  How often does this 
>sequence occur in other proteins???  Is the presence of this sequence enough
>to generate the chromphore and if it isn't how much of the surrounding protein
>do you need.
>	I say all, Ponzy (my boss) says find out...
>	Any help is appreciated.
>	Teddy
I believe the evidence for GFP itself in E. coli is that almost all of 
the sequence is necessary for fluorescence.  Only a few amino acids can
be deleted from either end without losing activity.  Also Roger Tsien's 
group has shown an effect of a mutation 100 amino acids away from the 
active site.


William J. Buikema        Phone: (312)702-1081   e-mail: hetman at uchicago.edu
Dept. Mol. Genet. & Cell Biol.                   /  / --- --- /|/|  /|  /| /
Univ. of Chicago, 920 E. 58th St.               /--/ /-   /  / / | /-| / |/
Chicago, IL 60637 USA       Fax: (312)702-3172 /  / /__  /  /    |/  |/  /

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