At 6:46 PM 95.10.23 +0000, McCormick's Lab. wrote:
>Does anyone have experience using the molecular probes Imagene green
>and/or Imagene red for plant tissue (not cell culture)? We are interested
>in information concerning the overlap of the fluorescence with endogenous
>molecules like chlorophyl and on the permeability of these GUS substrates
>into tissue.
I used Imagene green to detect GUS in Arabidopsis leaves two years ago.
I sprayed Imagene green to intact Arabidopsis plants.
I couldn't see any defference between GUS+ transgenic and GUS- WT.
The background of Imagene green was extremely high.
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Yasuo Niwa, Ph. D.
Laboratory of Plant Cell Technology
University of Shizuoka
52-1 Yada, Shizuoka 422, Japan
Phone&Fax: +81(Japan)-54-264-5582
e-mail: niwa at fns1.u-shizuoka-ken.ac.jp
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