Hello.
I am posting this for a friend who has the following problem.
: Texas red is a fluorescent dye whose excitation wavelength is 589 nm.
: This is bound to albumin and then put into serum. The resulting cocktail is
: then eletrophoresed to separate the plasma proteins from the indigenous
: albumin as well as the texas red labeled albumin. By using fluorescence I
: would like to measure the quantity of labeled albumin present.
: Is there a scanner that can read the whole gel and present the data to
: a PC on which I am running NIH Image or Image Grabber.
: If I can use a fluorescent scanner this will rapidly help to determine
: the quantity of texas red present. One consideration to think about is that
: albumin by itself will fluoresce but this is less than 589nm - the upper
: fluorescent reach of the serum proteins is about 450 nm.
:: Thank you for helping
:: Ian
Please reply to either gdavies at lshtm.ac.uk or i.maconochie at ic.ac.uk
Many thanks
Graham.
