gdavies at lshtm.ac.uk (Graham Davies) wrote:
>Hello.
>I am posting this for a friend who has the following problem.
>>: Texas red is a fluorescent dye whose excitation wavelength is 589 nm.
>: This is bound to albumin and then put into serum. The resulting cocktail is
>: then eletrophoresed to separate the plasma proteins from the indigenous
>: albumin as well as the texas red labeled albumin. By using fluorescence I
>: would like to measure the quantity of labeled albumin present.
>: Is there a scanner that can read the whole gel and present the data to
>: a PC on which I am running NIH Image or Image Grabber.
>: If I can use a fluorescent scanner this will rapidly help to determine
>: the quantity of texas red present. One consideration to think about is that
>: albumin by itself will fluoresce but this is less than 589nm - the upper
>: fluorescent reach of the serum proteins is about 450 nm.
>:>: Thank you for helping
>:>: Ian
>>Please reply to either gdavies at lshtm.ac.uk or i.maconochie at ic.ac.uk>>Many thanks
>>Graham.
WARNING, MESSAGE FROM A COMMERCIAL SOURCE!
Graham,
We, Molecular Dynamics, make several flat bed fluorescent scanners designed to image gels. Neither of our
instruments is optimal for Texas Red;
the FSI excites with 488 nm (e.g. FITC, Bodipy,...)
the Storm 860 System excites with either 450nm or 632nm (great for Cy5)
The Molecular Probes handbook suggests that TexasRed would be excited at ~7% efficiency with 488nm so if you
had a great deal of signal it might work.
Depending on the chemistry you are using to label the albumin a variety of reactive dyes are available
For more information check out http://www.mdyn.com
hope this helps,
david hanzel
hanzel at mdyn.com
