Newsnet users;
I have a reporter mouse line expressing EGFP to characterize a promoter. I am getting
good signal in subsets of cells in several tissues. I am using a HiQ FITC cube and am
getting quite a bit of autofluorescence in some tissue. What is the best way to deal
with this? (another filter?, an addition step to eliminate the autofluorescent?).
Also what is the best way to fix and mount tissue for GFP, I seem to be getting
photobleaching in Fluorosave. Thanks! jay at po.mri.montana.edu
