Jay,
I have noticed autofluorescence in live or fixed HT1080 fibroblast
cells that I use. This fluorescence can be distinguished from that of GFP
because it is still visible with the rhodamine cube. It must have a
broader spectrum of emmission than GFP. It's still a pain, but at least
you can differentiate GFP from bkg fluorescence.
MIKE
Mike Moser Tel: 206-616-7391
UW Department of Pathology FAX: 206-543-3644
Box 357470 moser at u.washington.edu
Seattle, WA 98195 http://weber.u.washington.edu/~moser
On 3 Dec 1996, jay brewster wrote:
> Newsnet users;
> I have a reporter mouse line expressing EGFP to characterize a promoter. I am getting
> good signal in subsets of cells in several tissues. I am using a HiQ FITC cube and am
> getting quite a bit of autofluorescence in some tissue. What is the best way to deal
> with this? (another filter?, an addition step to eliminate the autofluorescent?).
> Also what is the best way to fix and mount tissue for GFP, I seem to be getting
> photobleaching in Fluorosave. Thanks! jay at po.mri.montana.edu>>
