In article <4c1fv5$jrm at itssrv1.ucsf.edu>, Brian Wickes
<wickes at cgl.ucsf.edu> wrote:
> Has anyone had difficulty expressing GFP-1 in bluscript? How did you
> make your construct (using SK+ and GFP from pGFP-1) and was a longwave
> hand held UV light ok?
We found that only some of the colonies from the transformation gave very
high fluorescence. We did use a hand held UV light source to detect the
Fluorescence. It helps to have a dark room to see it. A nontransformed E.
coli control is good for comparison as well.
Brandt G. Cassidy
The Samuel Roberts Noble Foundation
e-mail BGCASSIDY@ noble.org