I work with GFP cDNA from one year and I have any fluorescence detected
when the construct with these threee cDNA (TU#58, TU#60 and TU#65) are
electroporated in human hematopoietic cell lines. The problems of
circularization of the chromophore or the non consensus ATG sequences
have been suggested.
Somebody have some experienced with these constructs?
Thanks.
