In Article <4cscnt$tme at pulp.ucs.ualberta.ca>, wklimke at gpu.srv.ualberta.ca
(William Klimke) wrote:
>Does GFP require an extraneous energy source to function, and if so, can this
>energy source (I'm assuming that it's ATP) be supplied for example in the
>media, or does it need to be supplied by the cell. I'm considering doing a
>fusion to an outer membrane protein, so it would not have a readily available
>source of energy.
>>Bill
>
GFP is fluorescent. In A. victoria, aequorin supplies the energy. In
heterologous systems, the energy comes from the light you use to illuminate
your cells.
Stu Kuhstoss
