delabess <delabess at lovelace.fr> wrote:
>> I work with GFP cDNA from one year and I have any fluorescence detected
> when the construct with these threee cDNA (TU#58, TU#60 and TU#65) are
> electroporated in human hematopoietic cell lines.
It is not clear whether or not you moved the cDNA from TU#58, TU60 &
TU#65 (which are all bacterial expression vectors) into a mammalian
expression vector. Assuming you did, do you have a strong promoter
such as CMV driving expression of GFP?
-= Paul =-
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