I'm doing GFP expression in mammalian cells microinjecting constructed
mRNA which contains 5'cap, GFP coding region and poly A track. But it
does not have Kozak sequence. I observed some fluorescence but wonder if
the signal could be increased. Somebody have information about
comparison of mRNA expression with Kozak sequence and without it in
mammalian cells?
Please let me know if you have any.
Sunjong Kwon
Dept. Biochemistry
Univ. Conn. Health Ctr.
e-mail: skwon at panda.uchc.edu
