IUBio

No GFP fluorescence?

David de Graaf BMGraaf at dapsas1.weizmann.ac.il
Thu Jan 18 07:20:45 EST 1996


In article <9601160038.AA07298 at bkns01.biken.osaka-u.ac.jp> Masaru Okabe,
okabe at biken.osaka-u.ac.jp writes:
>
>de Graaf> but GFP
>de Graaf>does not fluoresce when it is synthesized at 37.  
>
>In transgenic mice, GFP (and RS-GFP) is produce in the environment of 37 C
>or higher and fluorecent.
>
>Masaru Okabe

Read the recent TIBS review. It is much less fluorescent at 37. I was
aware of it being used as a reproter in plants, C> elegans and
Drosopphila, but not in mice. References?

David



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