long lasting probe for cell migration tracking?

bates dbates at hph.ucdavis.edu
Wed Jan 24 12:03:31 EST 1996

You could try the cell tracker dyes from Molecular Probes. Don't know about
three to four weeks, but three to four days is fine.
From: Brian Scott on Tue, Jan 23, 1996 5:20 PM
Subject: long lasting probe for cell migration tracking?
To: fluorpro at net.bio.net

Subject: long lasting label for tracking cell migration?


I'd like to know if anyone could recommend a long lasting label which would
allow us to track the migration of newborn cells within the rat CNS and
allow us to visualize the cells for in vitro recording at their final
locations.  The ideal situation would be to microinject the label into the
area of interest and wait for three to four weeks.  Then visualize the cells
in vitro with fluorescence and then do patch clamping when the cells are at
their final locations.  Has this been done before?  Can a fluorescent probe
last for this length of time in vivo?



Brian Scott            | "In other studies you go as far as others have gone
brians at interlog.com    |  before you, and there is nothing more to know; but
 Physiology student +  |  in scientific pursuit there is continual food for
 general science buff. |  discovery and wonder."  -  Victor Frankenstein  

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To: fluorpro at net.bio.net
From: brians at interlog.com (Brian Scott)
Subject: long lasting probe for cell migration tracking?
Date: 23 Jan 1996 17:52:27 -0500
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