Hello,
I now try to express GFP in organ cultures of rat brain slices.
We cannot use viral vectors, because of strict regulation in
our laboratory . Therefore, I transfected the plasmid DNA
by lipofection in combination with focal extracellular
injections or intracellular microinjections. However, no
successful expression of GFP has been obtained. Since
we could express GFP in dissociated cells, the problem
seems to be the transfection technique itself. I would greatly
appreciate it, if anyone would give suggestions or information
about transfection techniques to slice cultures.
Thanking you for your help in advance.
Masayoshi Okada, Ph. D.
E-mail masayoshi.okada at shionogi.co.jp
(+81) 6-331-8081
(+81) 6-332-6385 (FAX)
