I was wondering if anyone has developed a quantitative assay for GFP using
a fluorimeter? We are bombarding plant tissues and electroporating
protoplasts and would like to quantitate the level of GFP expression. We
thought that some form of cell lysis and fluorimitry might be a way to
quantitate the amount of GFP. Any ideas or other help with this would be
appreciated.
Brandt G. Cassidy
Assistant Staff Scientist
Plant Biology
The Samuel Roberts Noble Foundation
e-mail BGCASSIDY at noble.org
