I was wondering if anyone has developed a quantitative assay for GFP using
a fluorimeter? We are bombarding plant tissues and electroporating
protoplasts and would like to quantitate the level of GFP expression. We
thought that some form of cell lysis and fluorimitry might be a way to
quantitate the amount of GFP. Any ideas or other help with this would be
Brandt G. Cassidy
Assistant Staff Scientist
The Samuel Roberts Noble Foundation
e-mail BGCASSIDY at noble.org
You may try purifying GFP and reading known quantities to establish your
standards for the fluorimeter. Depending on the GFP you are using, the
fluorimeter may not be sensitive enough to give an accurate estimation of
GFP expression in the cells.
Life Technologies, Inc.
kle1058 at aol.com