I am directing this to any Drosophila people out there or others who have
had success at expressing GFP chimeric proteins under the control of the
I have successfully made a GFP construct with my protein and now I am
trying to analyze the expression in embryos and larva. Can anyone tell me
the details of how to examine the expression in early embryos, i.e. how
long should I heat shock, how long should I wait to look for expression and
fluorescence after heat shock, will the heat shock interfere with folding
of the protein, etc..
These are just a few of my questions so I am hoping that someone with
success will answer and enlighten me.
Thanks in advance!
Jennifer L. Hicks
Department of Biology, Box 1229
One Brookings Drive
St. Louis, MO 63130