Restriction sites in GFP. look here for hGFP.
INFMEDLAB
mamocell at INET.UNI-C.DK
Tue Jul 16 07:23:41 EST 1996
This is the restrictionmap of hGFP from phGFP (phGFP, CVU43284). hGFP:
826-1545 bp.
This is a search for many restriction sites using the NTI vector 3.0
program.
Gregers J. Gram, july 1996.
ScrFI
~~~~~~
NciI TaqI
~~~~~~ ~
MslI ScrFI
~~~~~~~~~~~ ~~~~~
NlaIII HpaII NlaIV EcoRII
~~~ ~~~~~ ~~~~~~~ ~~~~~
MslI DsaV BmyI DsaV
~~~~~~ ~~~~~~ ~~~~~~~ ~~~~~
HphI AluI BsaJI BanI BstNI
~~~~~ ~~~~~ ~~~~~~~ ~~~~~~~ ~~~~~
826 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
TACCACTCGT TCCCGCTCCT CGACAAGTGG CCCCACCACG GGTAGGACCA
MnlI HphI FokI
~~~~ ~~~~~ ~~~~~
TaqI HaeIII HpaII
~~~ ~~~~ ~~~~
AluI MaeII EaeI BspWI
~~~~ ~~~~ ~~~~~~ ~~~~~~~~~~~~
876 CGAGCTGGAC GGCGACGTGA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG
GCTCGACCTG CCGCTGCACT TGCCGGTGTT CAAGTCGCAC AGGCCGCTCC
MnlI
~~~~
BcgI AluI Eco57I BspWI
~~~~~~~~~~~~~ ~~~~ ~~~~~~ ~~~~~
926 GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC
CGCTCCCGCT ACGGTGGATG CCGTTCGACT GGGACTTCAA GTAGACGTGG
MnlI SfaNI BsgI
~~~~ ~~~~~~ ~~~~~~
ScrFI
~~~~~~
Sau96I
~~~~~
EcoRII
~~~~~~
DsaV
~~~~~~
BspWI BstNI
~~~~~~~~~~~~~~~~~~
HpaII BsiYI MslI
~~~~ ~~~~~~~~~~~~ ~~~~~~~~~~~
BsrFI Fnu4HI BsaJI MnlI
~~~~~~ ~~~~~~ ~~~~~~~ ~~~~~
BspWI AluI BmyI HaeIII MaeIII
~~~~~~ ~~~~~ ~~~~~~~ ~~~~ ~~~~~
976 ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCTTCACCTA
TGGCCGTTCG ACGGGCACGG GACCGGGTGG GAGCACTGGT GGAAGTGGAT
BbvI HphI
~~~~~~ ~~~~~
BspWI
~~~~~~~~~~~~
BspWI Fnu4HI Fnu4HI
~~~~~~~~~~~~ ~~~~~~ ~~~~~~
BsgI AciI NlaIII BbvI
~~~~~~ ~~~~~ ~~~~ ~~~~~~
1026 CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT
GCCGCACGTC ACGAAGTCGG CGATGGGGCT GGTGTACTTC GTCGTGCTGA
Eco57I
~~~~~~
ScrFI
~~~~~
MaeII
~~~~~
EcoRII
~~~~~
NlaIII DsaV HinP1I
~~~~ ~~~~~ ~~~~~
AciI BstNI CfoI
~~~~~ ~~~~~ ~~~~~
1076 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC
AGAAGTTCAG GCGGTACGGG CTTCCGATGC AGGTCCTCGC GTGGTAGAAG
MboII
~~
MboII MboII
~~~~~ ~~~~~
HinP1I
~~~~~
CfoI
~~~~~
BstUI HphI
~~~~~ ~~~~~
BsiYI
~~~~~~~~~~~~
AciI BsaJI TaqI
~~~~ ~~~~~~ ~~~~
1126 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
AAGTTCCTGC TGCCGTTGAT GTTCTGGGCG CGGCTCCACT TCAAGCTCCC
MboII MnlI MnlI
~~~ ~~~~ ~~~~
ScrFI
~~~~~~
MslI
~~~~~~~~~~~
HphI
~~~~~~
EcoRII
~~~~~~
DsaV TaqI
~~~~~~ ~~~~
BstNI SfaNI Eco57I TaqI
~~~~~~ ~~~~~~ ~~~~~~~ ~~~~
BsaJI AciI AluI SfaNI
~~~~~~~ ~~~~ ~~~~ ~~~~~
1176 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG
GCTGTGGGAC CACTTGGCGT AGCTCGACTT CCCGTAGCTG AAGTTCCTCC
MnlI
~~~~
ScrFI
~~~~~
EcoRII
~~~~~
DsaV
~~~~~
BstNI RsaI
~~~~~ ~~~~~
BsaJI BpmI
~~~~~~ ~~~~~~
BmyI AluI Csp6I MaeII
~~~~~~ ~~~~ ~~~~~ ~~
1226 ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC
TGCCGTTGTA GGACCCCGTG TTCGACCTCA TGTTGATGTT GTCGGTGTTG
FokI
~~~~~~
NlaIII
~~~~~
HaeIII MslI
~~~~ ~~~~~~~~~~~
MaeII EaeI MboII SfaNI HphI BstYI
~~ ~~~~~~ ~~~~~ ~~~~~ ~~~~~~ ~
1276 GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA
CAGATATAGT ACCGGCTGTT CGTCTTCTTG CCGTAGTTCC ACTTGAAGTT
DpnII Fnu4HI
~~~~ ~~~~~
DpnI BsgI
~~~~ ~~~~~~~
BstYI Fnu4HI BbvI
~~~~~ ~~~~~ ~~~~~
AciI TaqI BbvI AluI
~~~~ ~~~~ ~~~~~ ~~~~
1326 GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC
CTAGGCGGTG TTGTAGCTCC TGCCGTCGCA CGTCGAGCGG CTGGTGATGG
AlwI MnlI
~~~~~ ~~~~
Sau96I
~~~~~
NlaIV Fnu4HI
~~~~~~ ~~~~~
HaeIII Fnu4HI
~~~~ ~~~~~
1376 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC
TCGTCTTGTG GGGGTAGCCG CTGCCGGGGC ACGACGACGG GCTGTTGGTG
BbvI
~~~~~
BbvI
~~~~~
HinP1I
~~~~
DpnII
~~
DdeI DpnI
~~~~~ ~~
BmyI CfoI
~~~~~~~ ~~~~
AspHI AciI DdeI BstUI
~~~~~~~ ~~~~ ~~~~~ ~~~~
1426 TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA
ATGGACTCGT GGGTCAGGCG GGACTCGTTT CTGGGGTTGC TCTTCGCGCT
BsrI
~~~~~
ScrFI
~~~~~
NciI
~~~~~
HpaII
~~~~
DsaV
~~~~~
Fnu4HI DpnII
~~~~~~ ~~~~
NlaIII BsiYI
~~~~ ~~~~~~~~~~~~
DpnIISau96I MaeIII AciI DpnI
~~ ~~~~~~ ~~~~~ ~~~~~ ~~~~
DpnI AvaII BpmI AciI AlwI NlaIII
~~ ~~~~~~ ~~~~~~ ~~~~ ~~~~~ ~~
1476 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCACGGCA
AGTGTACCAG GACGACCTCA AGCACTGGCG GCGGCCCTAG TGAGTGCCGT
RsaI
~~~~
BsrGI
~~~~~~~
NlaIIIAluICsp6I
~~ ~~~~~~~~~
1526 TGGACGAGCT GTACAAGTAA
ACCTGCTCGA CATGTTCATT
General Description
molecule name: phGFP, CVU43284
molecule length: 4145 bp
fragment length: 720 bp
from: 826
to: 1545
Functional Map
CDS (1 signal)
hGFP, S65T
Start: 826 End: 1545
NCBI gi: 1151242
Modified_base (1 signal)
S65T
Start: 1021 End: 1023
Restriction Map
Acc65I (Asp718): 0 sites G/GTACC
AccI: 0 sites GT/MKAC
AccIII (BseAI, BspEI, MroI): 0 sites T/CCGGA
AciI: 8 sites C/CGC
N1: 1045
N2: 1086
N3: 1153
N4: 1192
N5: 1330
N6: 1443
N7: 1503
N8: 1506
AcsI (ApoI): 0 sites R/AATTY
AcyI (BsaHI): 0 sites GR/CGYC
AflII (BfrI): 0 sites C/TTAAG
AflIII: 0 sites A/CRYGT
AgeI (PinAI): 0 sites A/CCGGT
AluI: 8 sites AG/CT
N1: 847
N2: 880
N3: 952
N4: 985
N5: 1201
N6: 1249
N7: 1360
N8: 1534
Alw44I (ApaLI, SnoI): 0 sites G/TGCAC
AlwI: 2 sites GGATCNNNN/NN
N1: 1321 (on complementary strand)
N2: 1520
AlwNI: 0 sites CAGNNN/CTG
AosI (AviII, FspI): 0 sites TGC/GCA
ApaI: 0 sites GGGCC/C
AscI: 0 sites GG/CGCGCC
AseI (AsnI): 0 sites AT/TAAT
Asp700 (XmnI): 0 sites GAANN/NNTTC
AspEI (Eam1105I): 0 sites GACNNN/NNGTC
AspHI (BsiHKAI, HgiAI): 1 site GWGCW/C
N1: 1436
AspI (Tth111I): 0 sites GACN/NNGTC
AsuII (BstBI, Csp45I, NspV, SfuI): 0 sites TT/CGAA
AvaI: 0 sites C/YCGRG
AvaII: 1 site G/GWCC
N1: 1483
AvrII (BlnI): 0 sites C/CTAGG
BamHI: 0 sites G/GATCC
BanI: 1 site G/GYRCC
N1: 862
BanII: 0 sites GRGCY/C
BbrPI (PmaCI, PmlI): 0 sites CAC/GTG
BbsI (BpuAI): 0 sites GAAGACNN/NNNNN
BbvI: 6 sites GCAGCNNNNNNNN/NNNNN
N1: 972 (on complementary strand)
N2: 1078
N3: 1362
N4: 1369
N5: 1395 (on complementary strand)
N6: 1398 (on complementary strand)
BcgI: 1 site CGANNNNNNTGCNNNNNNNNNNNN/N
N1: 951
BclI: 0 sites T/GATCA
BfaI (MaeI): 0 sites C/TAG
BglI: 0 sites GCCNNNN/NGGC
BglII: 0 sites A/GATCT
BmyI (Bsp1286I): 4 sites GDGCH/C
N1: 867
N2: 996
N3: 1245
N4: 1436
BpmI: 2 sites CTGGAGNNNNNNNNNNNNNNNN/N
N1: 1271
N2: 1511
Bpu1102I (CelII, EspI): 0 sites GC/TNAGC
BsaAI: 0 sites YAC/GTR
BsaBI (MamI): 0 sites GATNN/NNATC
BsaI: 0 sites GGTCTCN/NNNNN
BsaJI: 5 sites C/CNNGG
N1: 855
N2: 995
N3: 1158
N4: 1182
N5: 1237
BsaWI: 0 sites W/CCGGW
BsgI: 3 sites GTGCAGNNNNNNNNNNNNNNNN/N
N1: 955 (on complementary strand)
N2: 1052
N3: 1376
BsiEI (McrI): 0 sites CGRY/CG
BsiWI: 0 sites C/GTACG
BsiYI (BslI): 3 sites CCNNNNN/NNGG
N1: 996
N2: 1159
N3: 1509
BsmAI: 0 sites GTCTCN/NNNNN
BsmFI: 0 sites GGGACNNNNNNNNNN/NNNNN
BsmI: 0 sites GAATGCN/N
Bsp120I: 0 sites G/GGCCC
BspDI (ClaI): 0 sites AT/CGAT
BspHI (RcaI): 0 sites T/CATGA
BspMI: 0 sites ACCTGCNNNN/NNNNN
BspWI (MwoI): 5 sites GCNNNNN/NNGC
N1: 918
N2: 978
N3: 991
N4: 1035
N5: 1044
BsrBI: 0 sites GAG/CGG
BsrDI: 0 sites GCAATGNN/N
BsrFI (Cfr10I): 1 site R/CCGGY
N1: 977
BsrGI (SspBI): 1 site T/GTACA
N1: 1536
BsrI: 1 site ACTGGN/N
N1: 1438 (on complementary strand)
BssHII: 0 sites G/CGCGC
Bst1107I: 0 sites GTA/TAC
BstEII: 0 sites G/GTNACC
BstNI (MvaI): 5 sites CC/WGG
N1: 872
N2: 997
N3: 1109
N4: 1184
N5: 1238
BstUI (FnuDII, MvnI, ThaI): 2 sites CG/CG
N1: 1155
N2: 1473
BstXI: 0 sites CCANNNNN/NTGG
BstYI (XhoII): 1 site R/GATCY
N1: 1326
Bsu36I: 0 sites CC/TNAGG
CfoI (HhaI): 3 sites GCG/C
N1: 1116
N2: 1157
N3: 1473
Csp6I: 2 sites G/TAC
N1: 1255
N2: 1537
DdeI: 2 sites C/TNAG
N1: 1430
N2: 1448
DpnI: 3 sites GA/TC
N1: 1328
N2: 1476
N3: 1514
DpnII (MboI, NdeII, Sau3AI): 3 sites N/GATCN
N1: 1326
N2: 1474
N3: 1512
DraI: 0 sites TTT/AAA
DraII (EcoO109I): 0 sites RG/GNCCY
DraIII: 0 sites CACNNN/GTG
DrdI: 0 sites GACNNNN/NNGTC
DsaI: 0 sites C/CRYGG
DsaV: 7 sites N/CCNGGN
N1: 854
N2: 870
N3: 995
N4: 1107
N5: 1182
N6: 1236
N7: 1508
EaeI: 2 sites Y/GGCCR
N1: 898
N2: 1287
EagI (EclXI, XmaIII): 0 sites C/GGCCG
EarI (Ksp632I): 0 sites CTCTTCN/NNNN
Ecl136II: 0 sites GAG/CTC
Eco47III: 0 sites AGC/GCT
Eco57I: 3 sites CTGAAGNNNNNNNNNNNNNNNN/N
N1: 980
N2: 1024 (on complementary strand)
N3: 1223
EcoNI: 0 sites CCTNN/NNNAGG
EcoRI: 0 sites G/AATTC
EcoRII: 5 sites N/CCWGGN
N1: 870
N2: 995
N3: 1107
N4: 1182
N5: 1236
EcoRV: 0 sites GAT/ATC
Esp3I: 0 sites CGTCTCN/NNNNN
Fnu4HI (ItaI): 8 sites GC/NGC
N1: 986
N2: 1045
N3: 1067
N4: 1351
N5: 1358
N6: 1409
N7: 1412
N8: 1506
FokI: 2 sites GGATGNNNNNNNNN/NNNNN
N1: 854 (on complementary strand)
N2: 1220 (on complementary strand)
HaeII: 0 sites RGCGC/Y
HaeIII: 4 sites GG/CC
N1: 900
N2: 1000
N3: 1289
N4: 1401
HgaI: 0 sites GACGCNNNNN/NNNNNN
HincII (HindII): 0 sites GTY/RAC
HindIII: 0 sites A/AGCTT
HinfI: 0 sites G/ANTC
HinP1I: 3 sites G/CGC
N1: 1114
N2: 1155
N3: 1471
HpaI: 0 sites GTT/AAC
HpaII (MspI): 4 sites C/CGG
N1: 855
N2: 918
N3: 978
N4: 1509
HphI: 6 sites GGTGANNNNNNNN/N
N1: 841
N2: 844 (on complementary strand)
N3: 1012 (on complementary strand)
N4: 1174
N5: 1198
N6: 1327
KasI: 0 sites G/GCGCC
KpnI: 0 sites GGTAC/C
KspI (SacII): 0 sites CCGC/GG
MaeII: 3 sites A/CGT
N1: 891
N2: 1104
N3: 1275
MaeIII: 2 sites N/GTNACN
N1: 1009
N2: 1498
MboII: 4 sites GAAGANNNNNNNN/N
N1: 1069 (on complementary strand)
N2: 1114 (on complementary strand)
N3: 1117 (on complementary strand)
N4: 1312
MfeI (MunI): 0 sites C/AATTG
MluI: 0 sites A/CGCGT
MluNI (MscI): 0 sites TGG/CCA
MnlI: 8 sites CCTCNNNNNNN/N
N1: 835 (on complementary strand)
N2: 916 (on complementary strand)
N3: 922 (on complementary strand)
N4: 1016
N5: 1153 (on complementary strand)
N6: 1165 (on complementary strand)
N7: 1216 (on complementary strand)
N8: 1336 (on complementary strand)
MseI (Tru9I): 0 sites T/TAA
MslI: 5 sites CAYNN/NNRTG
N1: 827
N2: 857
N3: 1007
N4: 1184
N5: 1313
MspA1I: 0 sites CMG/CKG
NaeI: 0 sites GCC/GGC
NarI: 0 sites GG/CGCC
NciI: 2 sites CC/SGG
N1: 856
N2: 1510
NcoI: 0 sites C/CATGG
NdeI: 0 sites CA/TATG
NgoMI: 0 sites G/CCGGC
NheI: 0 sites G/CTAGC
NlaIII: 6 sites NCATG/N
N1: 829
N2: 1063
N3: 1093
N4: 1288
N5: 1483
N6: 1528
NlaIV: 2 sites GGN/NCC
N1: 864
N2: 1402
NotI: 0 sites GC/GGCCGC
NruI: 0 sites TCG/CGA
NsiI: 0 sites ATGCA/T
NspI: 0 sites RCATG/Y
PacI: 0 sites TTAAT/TAA
PaeR7I (XhoI): 0 sites C/TCGAG
PflMI (Van91I): 0 sites CCANNNN/NTGG
PleI: 0 sites GAGTCNNNN/NN
PmeI: 0 sites GTTT/AAAC
Ppu10I: 0 sites A/TGCAT
PpuMI: 0 sites RG/GWCCY
Psp1406I: 0 sites AA/CGTT
PstI: 0 sites CTGCA/G
PvuI: 0 sites CGAT/CG
PvuII: 0 sites CAG/CTG
RsaI: 2 sites GT/AC
N1: 1256
N2: 1538
RsrII: 0 sites CG/GWCCG
SacI: 0 sites GAGCT/C
SalI: 0 sites G/TCGAC
SapI: 0 sites GCTCTTCN/NNNN
Sau96I: 3 sites G/GNCC
N1: 999
N2: 1400
N3: 1483
ScaI: 0 sites AGT/ACT
ScrFI: 7 sites CC/NGG
N1: 856
N2: 872
N3: 997
N4: 1109
N5: 1184
N6: 1238
N7: 1510
SexAI: 0 sites A/CCWGGT
SfaNI: 4 sites GCATCNNNNN/NNNNN
N1: 925 (on complementary strand)
N2: 1203
N3: 1218
N4: 1317
SfcI: 0 sites C/TRYAG
SfiI: 0 sites GGCCNNNN/NGGCC
SgrAI: 0 sites CR/CCGGYG
SmaI: 0 sites CCC/GGG
SnaBI: 0 sites TAC/GTA
SpeI: 0 sites A/CTAGT
SphI: 0 sites GCATG/C
SspI: 0 sites AAT/ATT
StyI: 0 sites C/CWWGG
SwaI: 0 sites ATTT/AAAT
TaqI: 5 sites T/CGA
N1: 876
N2: 1170
N3: 1197
N4: 1212
N5: 1341
TfiI: 0 sites G/AWTC
Tsp509I: 0 sites N/AATTN
XbaI: 0 sites T/CTAGA
XcmI: 0 sites CCANNNNN/NNNNTGG
XmaI: 0 sites C/CCGGG
AatI (StuI): 0 sites AGG/CCT
AatII: 0 sites GACGT/C
Annotations
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