Restriction sites in GFP
INFMEDLAB
mamocell at INET.UNI-C.DK
Tue Jul 16 07:23:28 EST 1996
This is the restrictionmap of GFP from pGFP (XXU17997B). GFP: 289-1005 bp.
This is a search for many restriction sites using the NTI vector 3.0
program.
Gregers J. Gram, july 1996.
BsrI Tsp509I
~~~~~ ~
MboII BpmI Tsp509I
~~~~~ ~~~~~~~ ~~~~~
289 ATGAGTAAAG GAGAAGAACT TTTCACTGGA GTTGTCCCAA TTCTTGTTGA
TACTCATTTC CTCTTCTTGA AAAGTGACCT CAACAGGGTT AAGAACAACT
BsmFI
~~~~~
Tsp509I
~~~~
Tsp509IHphI MseI BmyI AcsI HphI
~~~ ~~~~~~ ~~~~ ~~~~~~~~~~~~~~ ~~~~
339 ATTAGATGGT GATGTTAATG GGCACAAATT TTCTGTCAGT GGAGAGGGTG
TAATCTACCA CTACAATTAC CCGTGTTTAA AAGACAGTCA CCTCTCCCAC
MnlI
~~~~
Tsp509I
~~~~
HphI MseI
~~~~~ ~~~~
HphI AcsI BsrI
~ ~~~~~~ ~~~
389 AAGGTGATGC AACATACGGA AAACTTACCC TTAAATTTAT TTGCACTACT
TTCCACTACG TTGTATGCCT TTTGAATGGG AATTTAAATA AACGTGATGA
SfaNI
~~~~~
NlaIII
~~~~
NcoI
~~~~~~~
MluNI
~~~~~~~
HaeIII
~~~~~
EaeI
~~~~~~~
DsaI
~~~~~~~
BsaJI
~~~~~~~
BsrI StyI MaeIII
~~ ~~~~~~~ ~~~~~
439 GGAAAACTAC CTGTTCCATG GCCAACACTT GTCACTACTT TCTCTTATGG
CCTTTTGATG GACAAGGTAC CGGTTGTGAA CAGTGATGAA AGAGAATACC
DpnII
~~~~
DpnI
~~~~
NdeI NlaIII
~~~~~~~ ~~~~
489 TGTTCAATGC TTTTCAAGAT ACCCAGATCA TATGAAACGG CATGACTTTT
ACAAGTTACG AAAAGTTCTA TGGGTCTAGT ATACTTTGCC GTACTGAAAA
RsaI
~~~~
Csp6I
~~~~
NlaIII BsrGI
~~~~ ~~~~~~~
539 TCAAGAGTGC CATGCCCGAA GGTTATGTAC AGGAAAGAAC TATATTTTTC
AGTTCTCACG GTACGGGCTT CCAATACATG TCCTTTCTTG ATATAAAAAG
MaeII
~~~~
BsaAI
~~~~~~
BbrPI
~~~~~~
AflIII Eco57I HphI
~~~~~~ ~~~~~~~ ~~~~~
589 AAAGATGACG GGAACTACAA GACACGTGCT GAAGTCAAGT TTGAAGGTGA
TTTCTACTGC CCTTGATGTT CTGTGCACGA CTTCAGTTCA AACTTCCACT
TfiI
~~~~~
TaqI MseI
~~~~~ ~~~~~
MseI HinfI MseI DraI MboII
~~~~~ ~~~~~ ~~~~ ~~~~~~~~~~~~
639 TACCCTTGTT AATAGAATCG AGTTAAAAGG TATTGATTTT AAAGAAGATG
ATGGGAACAA TTATCTTAGC TCAATTTTCC ATAACTAAAA TTTCTTCTAC
AccI
~~~
Tsp509I Bst1107I
~~~~~ ~~~
689 GAAACATTCT TGGACACAAA TTGGAATACA ACTATAACTC ACACAATGTA
CTTTGTAAGA ACCTGTGTTT AACCTTATGT TGATATTGAG TGTGTTACAT
MseI
~~~~~
AccINlaIII TfiI HpaI
~~~ ~~~~ ~~~~~~ ~~~~~~~
Bst1107I HinfI HincII Tsp509I
~~~ ~~~~~~ ~~~~~~~ ~~~
739 TACATCATGG CAGACAAACA AAAGAATGGA ATCAAAGTTA ACTTCAAAAT
ATGTAGTACC GTCTGTTTGT TTTCTTACCT TAGTTTCAAT TGAAGTTTTA
Tsp509I MboII BfaI
~ ~~~~~ ~~~~
789 TAGACACAAC ATTGAAGATG GAAGCGTTCA ACTAGCAGAC CATTATCAAC
ATCTGTGTTG TAACTTCTAC CTTCGCAAGT TGATCGTCTG GTAATAGTTG
Tsp509I Sau96I
~~~~ ~~~~~
MfeI HaeIII
~~~~~~~ ~~~~
839 AAAATACTCC AATTGGCGAT GGCCCTGTCC TTTTACCAGA CAACCATTAC
TTTTATGAGG TTAACCGCTA CCGGGACAGG AAAATGGTCT GTTGGTAATG
DpnII
~~~~~
TaqI DpnI
~~~~ ~~~~~
AsuII BstYI DrdI
~~~~~~~~~~~~~ ~~~~~
889 CTGTCCACAC AATCTGCCCT TTCGAAAGAT CCCAACGAAA AGAGAGACCA
GACAGGTGTG TTAGACGGGA AAGCTTTCTA GGGTTGCTTT TCTCTCTGGT
BsmAI
~~~~~
AlwI BsaI
~~~~~~ ~~~~~~
MspA1I
~~~~~~
Sau96I Fnu4HI
~~~~~ ~~~~~
NlaIII AlwNI
~~~~ ~~~~~~~~~~
DrdI MaeIIIAluI NlaIII
~~~~~~~ ~~~~~~~~~~ ~~~~
AvaII PvuII NlaIII FokI
~~~~~ ~~~~~~ ~~~~ ~~
939 CATGGTCCTT CTTGAGTTTG TAACAGCTGC TGGGATTACA CATGGCATGG
GTACCAGGAA GAACTCAAAC ATTGTCGACG ACCCTAATGT GTACCGTACC
BbvI
~~~~~
FokI
~~~
989 ATGAACTATA CAAATAG
TACTTGATAT GTTTATC
General Description
molecule name: XXU17997B
molecule length: 3344 bp
fragment length: 717 bp
from: 289
to: 1005
Restriction Map
Acc65I (Asp718): 0 sites G/GTACC
AccI: 1 site GT/MKAC
N1: 738
AccIII (BseAI, BspEI, MroI): 0 sites T/CCGGA
AciI: 0 sites C/CGC
AcsI (ApoI): 2 sites R/AATTY
N1: 365
N2: 422
AcyI (BsaHI): 0 sites GR/CGYC
AflII (BfrI): 0 sites C/TTAAG
AflIII: 1 site A/CRYGT
N1: 611
AgeI (PinAI): 0 sites A/CCGGT
AluI: 1 site AG/CT
N1: 965
Alw44I (ApaLI, SnoI): 0 sites G/TGCAC
AlwI: 1 site GGATCNNNN/NN
N1: 911 (on complementary strand)
AlwNI: 1 site CAGNNN/CTG
N1: 968
AosI (AviII, FspI): 0 sites TGC/GCA
ApaI: 0 sites GGGCC/C
AscI: 0 sites GG/CGCGCC
AseI (AsnI): 0 sites AT/TAAT
Asp700 (XmnI): 0 sites GAANN/NNTTC
AspEI (Eam1105I): 0 sites GACNNN/NNGTC
AspHI (BsiHKAI, HgiAI): 0 sites GWGCW/C
AspI (Tth111I): 0 sites GACN/NNGTC
AsuII (BstBI, Csp45I, NspV, SfuI): 1 site TT/CGAA
N1: 911
AvaI: 0 sites C/YCGRG
AvaII: 1 site G/GWCC
N1: 943
AvrII (BlnI): 0 sites C/CTAGG
BamHI: 0 sites G/GATCC
BanI: 0 sites G/GYRCC
BanII: 0 sites GRGCY/C
BbrPI (PmaCI, PmlI): 1 site CAC/GTG
N1: 614
BbsI (BpuAI): 0 sites GAAGACNN/NNNNN
BbvI: 1 site GCAGCNNNNNNNN/NNNNN
N1: 952 (on complementary strand)
BcgI: 0 sites CGANNNNNNTGCNNNNNNNNNNNN/N
BclI: 0 sites T/GATCA
BfaI (MaeI): 1 site C/TAG
N1: 821
BglI: 0 sites GCCNNNN/NGGC
BglII: 0 sites A/GATCT
BmyI (Bsp1286I): 1 site GDGCH/C
N1: 363
BpmI: 1 site CTGGAGNNNNNNNNNNNNNNNN/N
N1: 336
Bpu1102I (CelII, EspI): 0 sites GC/TNAGC
BsaAI: 1 site YAC/GTR
N1: 614
BsaBI (MamI): 0 sites GATNN/NNATC
BsaI: 1 site GGTCTCN/NNNNN
N1: 927 (on complementary strand)
BsaJI: 1 site C/CNNGG
N1: 455
BsaWI: 0 sites W/CCGGW
BsgI: 0 sites GTGCAGNNNNNNNNNNNNNNNN/N
BsiEI (McrI): 0 sites CGRY/CG
BsiWI: 0 sites C/GTACG
BsiYI (BslI): 0 sites CCNNNNN/NNGG
BsmAI: 1 site GTCTCN/NNNNN
N1: 927 (on complementary strand)
BsmFI: 1 site GGGACNNNNNNNNNN/NNNNN
N1: 308 (on complementary strand)
BsmI: 0 sites GAATGCN/N
Bsp120I: 0 sites G/GGCCC
BspHI (RcaI): 0 sites T/CATGA
BspMI: 0 sites ACCTGCNNNN/NNNNN
BspWI (MwoI): 0 sites GCNNNNN/NNGC
BsrBI: 0 sites GAG/CGG
BsrDI: 0 sites GCAATGNN/N
BsrFI (Cfr10I): 0 sites R/CCGGY
BsrGI (SspBI): 1 site T/GTACA
N1: 565
BsrI: 2 sites ACTGGN/N
N1: 319
N2: 442
BssHII: 0 sites G/CGCGC
Bst1107I: 1 site GTA/TAC
N1: 739
BstEII: 0 sites G/GTNACC
BstNI (MvaI): 0 sites CC/WGG
BstUI (FnuDII, MvnI, ThaI): 0 sites CG/CG
BstXI: 0 sites CCANNNNN/NTGG
BstYI (XhoII): 1 site R/GATCY
N1: 916
Bsu36I: 0 sites CC/TNAGG
CfoI (HhaI): 0 sites GCG/C
ClaI (BspDI): 0 sites AT/CGAT
Csp6I: 1 site G/TAC
N1: 566
DdeI: 0 sites C/TNAG
DpnI: 2 sites GA/TC
N1: 516
N2: 918
DpnII (MboI, NdeII, Sau3AI): 2 sites N/GATCN
N1: 514
N2: 916
DraI: 1 site TTT/AAA
N1: 679
DraII (EcoO109I): 0 sites RG/GNCCY
DraIII: 0 sites CACNNN/GTG
DrdI: 1 site GACNNNN/NNGTC
N1: 941
DsaI: 1 site C/CRYGG
N1: 455
DsaV: 0 sites N/CCNGGN
EaeI: 1 site Y/GGCCR
N1: 458
EagI (EclXI, XmaIII): 0 sites C/GGCCG
EarI (Ksp632I): 0 sites CTCTTCN/NNNN
Ecl136II: 0 sites GAG/CTC
Eco47III: 0 sites AGC/GCT
Eco57I: 1 site CTGAAGNNNNNNNNNNNNNNNN/N
N1: 639
EcoNI: 0 sites CCTNN/NNNAGG
EcoRI: 0 sites G/AATTC
EcoRII: 0 sites N/CCWGGN
EcoRV: 0 sites GAT/ATC
Esp3I: 0 sites CGTCTCN/NNNNN
Fnu4HI (ItaI): 1 site GC/NGC
N1: 966
FokI: 1 site GGATGNNNNNNNNN/NNNNN
N1: 1001
HaeII: 0 sites RGCGC/Y
HaeIII: 2 sites GG/CC
N1: 460
N2: 861
HgaI: 0 sites GACGCNNNNN/NNNNNN
HincII (HindII): 1 site GTY/RAC
N1: 778
HindIII: 0 sites A/AGCTT
HinfI: 2 sites G/ANTC
N1: 654
N2: 768
HinP1I: 0 sites G/CGC
HpaI: 1 site GTT/AAC
N1: 778
HpaII (MspI): 0 sites C/CGG
HphI: 4 sites GGTGANNNNNNNN/N
N1: 359
N2: 398
N3: 404
N4: 647
KasI: 0 sites G/GCGCC
KpnI: 0 sites GGTAC/C
KspI (SacII): 0 sites CCGC/GG
MaeII: 1 site A/CGT
N1: 613
MaeIII: 2 sites N/GTNACN
N1: 469
N2: 958
MboII: 3 sites GAAGANNNNNNNN/N
N1: 314
N2: 695
N3: 815
MfeI (MunI): 1 site C/AATTG
N1: 849
MluI: 0 sites A/CGCGT
MluNI (MscI): 1 site TGG/CCA
N1: 460
MnlI: 1 site CCTCNNNNNNN/N
N1: 376 (on complementary strand)
MseI (Tru9I): 6 sites T/TAA
N1: 354
N2: 420
N3: 648
N4: 662
N5: 678
N6: 777
MslI: 0 sites CAYNN/NNRTG
MspA1I: 1 site CMG/CKG
N1: 965
NaeI: 0 sites GCC/GGC
NarI: 0 sites GG/CGCC
NciI: 0 sites CC/SGG
NcoI: 1 site C/CATGG
N1: 455
NdeI: 1 site CA/TATG
N1: 519
NgoMI: 0 sites G/CCGGC
NheI: 0 sites G/CTAGC
NlaIII: 7 sites NCATG/N
N1: 459
N2: 533
N3: 553
N4: 748
N5: 943
N6: 983
N7: 988
NlaIV: 0 sites GGN/NCC
NotI: 0 sites GC/GGCCGC
NruI: 0 sites TCG/CGA
NsiI: 0 sites ATGCA/T
NspI: 0 sites RCATG/Y
PacI: 0 sites TTAAT/TAA
PflMI (Van91I): 0 sites CCANNNN/NTGG
PleI: 0 sites GAGTCNNNN/NN
PmeI: 0 sites GTTT/AAAC
Ppu10I: 0 sites A/TGCAT
PpuMI: 0 sites RG/GWCCY
Psp1406I: 0 sites AA/CGTT
PstI: 0 sites CTGCA/G
PvuI: 0 sites CGAT/CG
PvuII: 1 site CAG/CTG
N1: 965
RsaI: 1 site GT/AC
N1: 567
RsrII: 0 sites CG/GWCCG
SacI: 0 sites GAGCT/C
SalI: 0 sites G/TCGAC
SapI: 0 sites GCTCTTCN/NNNN
Sau96I: 2 sites G/GNCC
N1: 860
N2: 943
ScaI: 0 sites AGT/ACT
ScrFI: 0 sites CC/NGG
SexAI: 0 sites A/CCWGGT
SfaNI: 1 site GCATCNNNNN/NNNNN
N1: 385 (on complementary strand)
SfcI: 0 sites C/TRYAG
SfiI: 0 sites GGCCNNNN/NGGCC
SgrAI: 0 sites CR/CCGGYG
SmaI: 0 sites CCC/GGG
SnaBI: 0 sites TAC/GTA
SpeI: 0 sites A/CTAGT
SphI: 0 sites GCATG/C
SspI: 0 sites AAT/ATT
StyI: 1 site C/CWWGG
N1: 455
SwaI: 0 sites ATTT/AAAT
TaqI: 2 sites T/CGA
N1: 657
N2: 911
TfiI: 2 sites G/AWTC
N1: 654
N2: 768
Tsp509I: 7 sites N/AATTN
N1: 327
N2: 338
N3: 365
N4: 422
N5: 707
N6: 786
N7: 849
XbaI: 0 sites T/CTAGA
XcmI: 0 sites CCANNNNN/NNNNTGG
XhoI (PaeR7I): 0 sites C/TCGAG
XmaI: 0 sites C/CCGGG
AatI (StuI): 0 sites AGG/CCT
AatII: 0 sites GACGT/C
Annotations
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