This is a reply to Karen Guterman's recent query about whether anyone had
targeted GFP to mitochondria and Mark Zylka's citation of a 1995 paper by
Rizzuto et al.
A yet more recent paper on mitochondrially targeted GFP from the Padova
group was Rizzuto et al (1996) Double labeling of subcellular structures
with organelle-targeted GFP mutants in vivo. Current Biology 6: 183-188.
Here they described mitochondrial targeting of S65T GFP as well as of the
brightest blue mutant of GFP, which was described in the companion paper
Current Biology 6: 178-182 (1996). So GFP seems to work very well in
Roger Y. Tsien
Howard Hughes Medical Institute and Univ. California, San Diego
Tel: (+1)619-534-4891, fax (+1)619-534-5270