Dear Dr. Christa Baumstark-Khan,
Actually, we have worked with the "Affymax cycle 3 mutant." It does
have somewhat stronger fluorescence than the wild-type GFP when
excited in the UV (i.e., at 395nm). However, it is less intense than the
red-shifted GFP variants (e.g., S65T, EGFP) when excited at 488nm. It's
utility depends on your application. For example, if you wanted to excite
with a hand-held uv light, the Affymax cycle 3 mutant would be useful.
However, if you planned on doing fluorescence microscopy with FITC
filters or FACS, one of the red-shifted GFP mutants would be a better
choice. We have just introduced the Affymax GFP variant, as a product
called GFPuv, for researchers who want to use a UV excitation for
detection in their experiments.
> Paul Diehl, Ph.D.
> CLONTECH Laboratories, Palo Alto
> ph: 800-662-2566 x1154
> fx: 415-424-0579
> pauld at clontech.com