Question,
Who has experience with recombinant animal virus expressing GFP, to
be able to localize the virus in organ sections, as an alternative
for Lac-Z ?. Until now I haven't found articles dealing with this
matter, except for a plant virus (Plant-J. 7(6), 1995). Are low
amounts of virus in a cell really critical for detection of GFP
expression?. Can this significantly be improved by: a strong
promoter,enhancer region/ GFP mutants yielding greater brightness/
localization of GFP to subcellular compartments ??. Maybe I missed a
previous discussion on this subject, being just recently introduced
to this newsgroup. Experiences and suggestions are very welcome !
Rien Blok
